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      • Complete nitrogen removal by simultaneous nitrification and denitrification in flat-panel air-cathode microbial fuel cells treating domestic wastewater

        Park, Younghyun,Park, Seonghwan,Nguyen, Van Khanh,Yu, Jaecheul,Torres, Cé,sar I.,Rittmann, Bruce E.,Lee, Taeho Elsevier 2017 Chemical engineering journal Vol.316 No.-

        <P><B>Abstract</B></P> <P>Microbial fuel cells (MFCs) can treat organic compounds from domestic wastewater without aeration, but an additional procedure is required to remove nitrogen. This study developed a flat-panel air-cathode MFC (FA-MFC) that was comprised of five MFC units connected in series and operated to remove organic and nitrogen compounds from domestic wastewater with a short hydraulic retention time (HRT) of 2.5h. During eight months of operation, the removal efficiencies of chemical oxygen demand (COD) and total nitrogen (TN) increased, reaching 85% and 94%, respectively, and the effluent COD and TN concentrations were 20.7±2.5mg/L and 1.7±0.1mg/L, respectively. The greatest removals of COD and TN were in the first and second unit (0.62kg-N/m<SUP>3</SUP>/d of TN removal rate). The FA-MFC system allowed simultaneous removals of COD and TN from domestic wastewater, although it led to minimal power output (6.3W/m<SUP>3</SUP> in the first unit). Because any abiotic ammonia loss was not found under the supplied potential of∼1.1V at a short HRT of 30min, the biological nitrogen removal was thought as a dominant mechanism for TN removal in the FA-MFCs. Microbial community analysis revealed that, near the cathode, <I>Nitrosomonas</I>-like strains contributed to nitrification and <I>Nitratireductor</I>-like strains led to denitrification. <I>Acidovorax</I>-like strains, known for their metabolic diversity, were ubiquitous and appeared to contribute to organics and nitrogen removal in anode and cathode biofilms. This study provides proof of concept that the FA-MFC system has a promise for energy sustainable wastewater treatment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> FA-MFC system for domestic wastewater treatment was operated during 8months. </LI> <LI> FA-MFCs could successfully satisfy COD and TN discharge limits at an HRT of∼2.5h. </LI> <LI> FA-MFC system showed a significantly high TN removal rate up to 0.62kg-N/m<SUP>3</SUP>/d. </LI> <LI> <I>Nitrosomonas</I>, <I>Nitratireductor</I> and <I>Acidovorax</I> spp. contributed to nitrogen removal. </LI> <LI> FA-MFC system has a promise for energy-sustainable domestic wastewater treatment. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • KCI등재

        Competition/antagonism associations of biofilm formation among Staphylococcus epidermidis Agr groups I, II, and III

        Sergio Martínez-García,sar I. Ortiz-García,Marisa Cruz-Aguilar,Juan Carlos Zenteno,José Martin Murrieta-Coxca,Sonia Mayra Pérez-Tapia,Sandra Rodríguez-Martínez,Mario E. Cancino-Diaz,Juan C. Cancino- 한국미생물학회 2019 The journal of microbiology Vol.57 No.2

        Staphylococci have quorum-sensing (QS) systems that enable cell-to-cell communication, as well as the regulation of numerous colonization and virulence factors. The accessory gene regulator (Agr) operon is one of the Staphylococcus genus QS systems. Three groups (I, II, and III) are present in Staphylococcus epidermidis Agr operon. To date, it is unknown whether Agr groups can interact symbiotically during biofilm development. This study analyzed a symbiotic association among Agr groups during biofilm formation in clinical and commensal isolates. Different combinations among Agr group isolates was used to study biofilm formation in vitro and in vivo (using a mouse catheter-infection model). The analysis of Agr groups were also performed from samples of human skin (head, armpits, and nostrils). Different predominant coexistence was found within biofilms, suggesting symbiosis type. In vitro, Agr I had a competition with Agr II and Agr III. Agr II had a competition with Agr III, and Agr II was an antagonist to Agr I and III when the three strains were combined. In vivo, Agr II had a competition to Agr I, but Agr I and II were antagonists to Agr III. The associations found in vitro and in vivo were also found in different sites of the skin. Besides, other associations were observed: Agr III antagonized Agr I and II, and Agr III competed with Agr I and Agr II. These results suggest that, in S. epidermidis, a symbiotic association of competition and antagonism occurs among different Agr groups during biofilm formation.

      • SCIESCOPUSKCI등재

        Effects of Combination of Nitrate with ${\beta}$1-4 Galacto-oligosaccharides and Yeast (Candida kefyr) on Methane Emission from Sheep

        Sar, C.,Santoso, B.,Gamo, Y.,Kobayashi, T.,Shiozaki, S.,Kimura, K.,Mizukoshi, H.,Arai, I.,Takahashi, J. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.1

        The objective of the present study was to determine whether ${\beta}$1-4 galacto-oligosaccharides (GOS) and Candida kefyr combined with nitrate as manipulators could suppress rumen methanogenesis without nitrate poisoning in sheep. Four rumen fistulated wethers were allocated to a $4{\times}4$ Latin square design. Nitrate (1.3 g $NaNO_3$ $Kg^{-0.75}$body weight) with and without GOS and Candida kefyr were administered into the rumen through fistula as a single dose 30 min after the morning meal. GOS and Candida kefyr were supplemented by sprinkling onto the feed and through rumen fistula, respectively. The four treatments consisted of saline, nitrate, nitrate plus GOS and nitrate plus GOS plus Candida kefyr. Physiological saline was used as the control treatment. Compared to saline treatment, the administration of nitrate alone resulted in a very marked decrease in rumen methanogenesis and an increase in rumen and plasma nitrite production and blood methaemoglobin formation consequently causing a decline in oxygen consumption, carbon dioxide production and metabolic rate. When compared to nitrate alone, the simultaneous administration of nitrate with GOS decreased nitrite accumulation in rumen and plasma and nitrate-induced methaemoglobin, while retaining low methane production. However, GOS could not fully restore metabolic parameters reduced by nitrate. When compared to the simultaneous administration of nitrate with GOS, the simultaneous administration of nitrate with GOS plus Candida kefyr lowered rumen methanogenesis to a negligible level, but did not decrease rumen and plasma nitrite accumulation as well as blood methaemoglobin formation. Thus, these results suggest that combination of nitrate with GOS may be a potent manipulator to suppress rumen methanogenesis with abating the hazards of nitratenitrite toxicity in ruminants.

      • SCIESCOPUSKCI등재

        Study of the methane flow influence in the micro‑tribology behavior of DLC coatings deposited by PECVD: a Raman analysis

        C?sar Daniel Rivera‑Tello,Francisco Javier Flores‑Ruiz,M. Flores,O. Jimenez,I. Far?as,J. Oseguera‑Pe?a 한국탄소학회 2021 Carbon Letters Vol.31 No.1

        This paper presents a Raman spectroscopy study of the influence of methane flow on the micro-tribological behavior of diamond-like carbon coatings deposited with an industrial plasma-enhanced chemical vapor deposition system. Results have shown a direct relationship between the methane flow and thickness of the coatings. The analysis of the Raman spectra and deposition parameters allowed establishing the influence of H content with the methane flow, the disorder level and estimation of the sp3 fraction on the carbon coatings. The micro-tribology tests showed a strong dependence of the wear resistance and hardness with Raman parameters. The coating deposited at 72-sccm methane flow presented a thickness of 1.7 μm and a sp3 fraction of 0.33. This sp3 fraction gave rise to a hardness of 24 GPa and an excellent wear resistance of 3.3 × 10–6 mm3 N?1 mm?1 for this DLC coating. Wear tests showed a swelling in the wear profiles on this coating, which was associated with the occurrence of a re-hybridization process.

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