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- 저자 : habib omer (검색결과 3 건)
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Habib, Omer,Habib, Gizem,Moon, Sung-Hwan,Hong, Ki-Sung,Do, Jeong Tae,Choi, Youngsok,Chang, Sung Woon,Chung, Hyung-Min Korean Society for Molecular and Cellular Biology 2014 Molecules and cells Vol.37 No.1
Induced pluripotent stem cells (iPSCs) are capable of unlimited self-renewal and can give rise to all three germ layers, thereby providing a new platform with which to study mammalian development and epigenetic reprogramming. However, iPSC generation may result in subtle epigenetic variations, such as the aberrant methylation of the Dlk1-Dio3 locus, among the clones, and this heterogeneity constitutes a major drawback to harnessing the full potential of iPSCs. Vitamin C has recently emerged as a safeguard to ensure the normal imprinting of the Dlk1-Dio3 locus during reprogramming. Here, we show that vitamin C exerts its effect in a manner that is independent of the reprogramming kinetics. Moreover, we demonstrate that reprogramming cells under 2i conditions leads to the early upregulation of Prdm14, which in turn results in a highly homogeneous population of authentic pluripotent colonies and prevents the abnormal silencing of the Dlk1-Dio3 locus.
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habib omer,Gizem Habib,문성환,홍기성,도정태,Youngsok Choi,장성운,정형민 한국분자세포생물학회 2014 Molecules and cells Vol.37 No.1
Induced pluripotent stem cells (iPSCs) are capable of unlimited self-renewal and can give rise to all three germ layers, thereby providing a new platform with which to study mammalian development and epigenetic reprogramming. However, iPSC generation may result in subtle epigenetic variations, such as the aberrant methylation of the Dlk1-Dio3 locus, among the clones, and this heterogeneity constitutes a major drawback to harnessing the full potential of iPSCs. Vitamin C has recently emerged as a safeguard to ensure the normal imprinting of the Dlk1-Dio3 locus during reprogramming. Here, we show that vitamin C exerts its effect in a manner that is independent of the reprogramming kinetics. Moreover, we demonstrate that reprogramming cells under 2i conditions leads to the early upregulation of Prdm14, which in turn results in a highly homogeneous population of authentic pluripotent colonies and prevents the abnormal silencing of the Dlk1-Dio3 locus.
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Applications of CRISPR technologies to the development of gene and cell therapy
Chul-Sung Park,Omer Habib,Younsu Lee,Junho K. Hur 생화학분자생물학회 2024 BMB Reports Vol.57 No.1
Advancements in gene and cell therapy have resulted in noveltherapeutics for diseases previously considered incurable orchallenging to treat. Among the various contributing technologies,genome editing stands out as one of the most crucialfor the progress in gene and cell therapy. The discovery ofCRISPR (Clustered Regularly Interspaced Short PalindromicRepeats) and the subsequent evolution of genetic engineeringtechnology have markedly expanded the field of target-specificgene editing. Originally studied in the immune systems ofbacteria and archaea, the CRISPR system has demonstratedwide applicability to effective genome editing of various biologicalsystems including human cells. The development ofCRISPR-based base editing has enabled directional cytosine-tothymineand adenine-to-guanine substitutions of select DNAbases at the target locus. Subsequent advances in prime editingfurther elevated the flexibility of the edit multiple consecutivebases to desired sequences. The recent CRISPR technologiesalso have been actively utilized for the development ofin vivo and ex vivo gene and cell therapies. We anticipate thatthe medical applications of CRISPR will rapidly progress to provideunprecedented possibilities to develop novel therapeuticstowards various diseases.
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