Chemical and Fatty Acid Composition of Longissimus Muscle of Crossbred Bulls Finished in Feedlot

do Prado, Ivanor Nunes,de Oliveira, Adriana Nery,Pizzi Rotta, Polyana,Perotto, Daniel,do Prado, Rodolpho Martin,Silva, Roberio Rodrigues,de Souza, Nilson Evelazio,Moletta, Jose Luiz Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.7

This work was carried out to study the chemical and fatty acid composition of Longissimus muscle (LM) of crossbred young bulls finished in a feedlot. After weaning (at 8 months old), the bulls were kept in a feedlot for 180 days. The bulls were kept in individual pens and fed (twice daily) with corn silage, soybean hulls, cracked corn, limestone, urea and mineral salt. The bulls were slaughtered with a final weight of 464 kg. Forty bulls were used: 10 Caracu (CAR), 10 Canchim (CAN), 10 Caracu vs. Charolais (CCH) and 10 Canchim vs. Aberdeen Angus (CAA). The percentages of moisture, ash, crude protein, total lipids, as well as the fatty acid composition, were measured in the LM. The moisture percentage was lower (p<0.05) for bulls from CAA genetic group (71.2%) in comparison to bulls from CAR (74.2%), CAN (74.9%) and CCH (74.7%) genetic groups. On the other hand, there was no difference (p>0.05) among bulls from CAR, CAN and CCH genetic groups. Ash percentage was lower (p<0.05) for CAR bulls (0.96%) in comparison with the other genetic groups. There was no difference (p>0.05) among CAN, CCH and CAA genetic groups. Similarly, there was no difference (p>0.05) in crude protein among the different genetic groups. Total lipids percentage was higher (p<0.05) for CAA bulls (5.35%) and lower (p<0.05) for CAN (1.85%) and CCH (1.41%) genetic groups. Genetic group has little effect on the fatty acid composition of Longissimus muscle of bulls. However, CLA (C 18:2 c-9 t-11) percentage was higher (p<0.05) for CAR (0.33%) and CCH (0.37%) in comparison to CAN (0.27%) and CAA (0.29%) genetic groups. Saturated, monounsaturated and polyunsaturated fatty acids, n-6 and n-3 percentages did not differ (p>0.05) among genetic groups. PUFA/SFA ratio ranged from 0.10 to 0.15, with no difference (p>0.05) among genetic groups. Similarly, n-6/n-3 ratio ranged from 12.6 to 16.3, without difference (p>0.05) among genetic groups.

Carcass Characteristics, Chemical Composition and Fatty Acid Profile of the Longissimus Muscle of Bulls (Bos taurus indicus vs. Bos taurus taurus) Finished in Pasture Systems

do Prado, Ivanor Nunes,Aricetti, Juliana Aparecida,Rotta, Polyana Pizzi,do Prado, Rodolpho Martin,Perotto, Daniel,Visentainer, Jesui Vergilio,Matsushita, Makoto Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.10

This experiment was carried out to evaluate the carcass characteristics, chemical composition and fatty acid profile of the Longissimus muscle (LM) of three cattle genetic groups (Purunã, PUR, 11; 1/2 Purunã vs. 1/2 British, PUB, 6 and 1/2 Charolais vs. 1/2 Caracu, CHC, 10) finished in pasture systems. The field work took place at the Lapa Research Farm of the Agronomic Institute of Paraná, in the city of Lapa, south Brazil. The animals were fed during the winter with corn silage, cottonseed meal, cracked corn, urea, limestone and mineral salts as sources of protein, as well as an energy supplement, in pasture systems of Brachiaria decumbens Stapf. The animal groups were slaughtered at 20 months of age, at 50122.6 kg live weight. CHC bulls had higher (p<0.05) final weight than PUR and PUB bulls. Hot carcass weight was similar (p>0.10) between PUR and PUB. Hot carcass dressing percentage was higher (p<0.05) for PUB bulls than for PUR and CHC bulls. On the other hand, hot carcass dressing percentage was similar (p>0.05) between PUR and CHC bulls. Fat thickness was similar (p>0.10) among all genetic groups. However, the Longissimus area of CHC bulls was greater (p<0.05) than in PUR and PUB genetic groups. The genetic groups did not affect (p>0.10) the marbling of Longissimus. There was no observed difference (p>0.10) in moisture, ash, crude protein and total cholesterol contents among the three genetic groups. On the other hand, the total lipid percentage was higher (p<0.05) for the PUB genetic group in comparison with PUR and CHC. CLA percentage was highest for PUR animals. However, total CLA amounts were not altered by the different genetic groups.

Carcass Characteristics and Chemical Composition of the Longissimus Muscle of Puruna and 1/2 Puruna vs. 1/2 Canchin Bulls Meat Quality of Bulls

Nunes do Prado, Ivanor,Rotta, Polyana Pizzi,Martin do Prado, Rodolpho,Visantainer, Jesui Vergilio,Moletta, Jose Luis,Perotto, Daniel Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.9

This work was conducted to study the performance, carcass characteristics, chemical composition and fatty acid profile of the Longissimus muscle (LM) of first-generation Purunã (n = 7, PUR1), second-generation Purunã (n = 9, PUR2), and 1/2 Puruna vs. 1/2 Canchin (n = 13, PUCA) bulls finished in a feedlot. The animals averaged 18 months of age at the beginning of the experiment. The animals were kept in a feedlot from 18 to 22 months of age. They were fed twice a day with corn silage ad libitum, along with soybean meal, cracked corn, urea, limestone and mineral salt. PUR1 bulls had greater (p<0.05) final weight (496.0 kg) than PUCA bulls (449.2 kg). However, there was no difference (p>0.05) between PUR1 and PUR2 (472.0 kg), and between PUR2 and PUCA. Hot carcass weight was similar (p>0.05) among the different genetic groups. Hot carcass dressing percentage was similar (p>0.10) between PUR1 (50.4%) and PUCA (53.8%), and higher (p<0.10) than PUR2 (48.6%). Cushion thickness was greater (p<0.05) in PUCA bulls (27.12 cm). The other parameters were similar (p<0.05) among the three genetic groups. Total lipid and cholesterol contents were higher (p<0.01) in PUR2. Moisture, ash and crude protein contents were similar (p>0.05) among the three genetic groups. Saturated fatty acid (SFA) levels were higher (p<0.10) in PUR1 and PUR2 (51.58% and 50.41%, respectively). Polyunsaturated fatty acid levels were higher (p<0.05) in PUR1 and PUCA (6.50% and 8.29%, respectively). N-6, n-3 and PUFA/SFA ratios were higher (p<0.05) in PUCA. MUFA and n-6/n-3 ratios were similar (p>0.05) among the different genetic groups.

Carcass Composition and Cuts of Bulls and Steers Fed with Three Concentrate Levels in the Diets

do Prado, Ivanor Nunes,Passetti, Rodrigo Augusto Cortez,Rivaroli, Dayane Cristina,Ornaghi, Mariana Garcia,de Souza, Kennyson Alves,Carvalho, Camila Barbosa,Perotto, Daniel,Moletta, Jose Luiz Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

In this paper, weight, carcass dressing, weights of the primary cuts, weights of the physical components of the primary cuts, and weights of the main commercial cuts of 66 $Purun{\tilde{a}}$ animals, of which 33 were bulls and 33 were steers were evaluated. These animals, with an average age of 19 months at the beginning of the experiment, were finished in a feedlot system during 116 days, and were fed with diets containing three levels of concentrate (0.8%, 1.1%, and 1.4% of body weight). The concentrate was formulated with 25% soybean meal, 73% ground corn grain, 1% of a mineral mix, and 1% of limestone. The interaction between sexual groups and the concentrate level was not significant for any of the variables. Likewise, no effect of the concentrate level was detected on the same variable traits. The bulls demonstrated higher hot carcass weights (265.1 vs 221.7 kg) and a higher proportion of forequarter (38.4% vs 36.1%) however the steers presented with higher proportions of side (19.7% vs 18.5%) and hindquarter (44.2% vs 43.1%). The bulls produced higher yields of muscle in the three primary cuts, there by resulting in higher yields of edible portions of the carcass. The bulls also produced higher weights of tenderloin, knuckle, topside, flat, eye round, rump, and rump cover. The finishing of young bulls in feedlot is to be recommended, since the animals produce carcasses with higher amounts of edible meat and higher yields of commercial cuts, thus allowing for a better price for the carcass. Low concentrate level could be used due to the lower cost of production for farmers.

Animal Performance and Carcass Characteristics of Bulls (1/2 Purunã vs 1/2 Canchim) Slaughtered at 16 and 22 Months Old, and Three Different Weights

Prado, Ivanor Nunes Do,Eiras, Carlos Emanuel,Fugita, Carlos Alberto,Passetti, Rodrigo Augusto Cortez,Ornaghi, Mariana Garcia,Rivaroli, Dayane Cristina,Pinto, Adriana Aparecida,Moletta, Jose Luiz Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.5

Current study aimed to evaluate the performance of bulls (1/2 Purun$\tilde{a}$ vs 1/2 Canchim) slaughtered at two ages and three different weights. One hundred and thirteen bulls were divided into two slaughter ages (16 and 22 months) and three different slaughter weights (light, 422 kg; medium, 470 kg; and heavy, 550 kg). The body weight was higher for bulls slaughtered at 16 months. Daily gain, carcass weight and dressing were higher for bulls slaughtered at 16 months. Feed intake was higher for bulls slaughtered at 22 months although feed efficiency was better for bulls slaughtered at 16 months. Carcass characteristics were better for bulls slaughtered at 16 months. The percentages of muscle, fat and bone and meat characteristics were similar between two slaughter ages. Feed intake and animal performance was lower for lighter animals. Feed conversion and carcass dressing were similar in the three slaughter weights. Muscle percentage was higher for heavier animals but fat and bone percentages were lower. Slaughter weight had no effect on meat characteristics.

The effects of non-thermal plasma and conventional treatments on the bond strength of fiber posts to resin cement

do Prado, Maira,da Silva, Eduardo Moreira,Marques, Juliana das Neves,Gonzalez, Caroline Brum,Simao, Renata Antoun The Korean Academy of Conservative Dentistry 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: This study compared the effect of hexamethyldisiloxane (HMDSO) and ammonia ($NH_3$) plasmas on the bond strength of resin cement to fiber posts with conventional treatments. Materials and Methods: Sixty-five fiber posts were divided into 5 groups: Control (no surface treatment); $H_2O_2$ (24% hydrogen peroxide for 1 min); Blasting (blasting with aluminum oxide for 30 sec); $NH_3$ ($NH_3$ plasma treatment for 3 min); HMDSO (HMDSO plasma treatment for 15 min). After the treatments, the Ambar adhesive (FGM Dental Products) was applied to the post surface (n = 10). The fiber post was inserted into a silicon matrix that was filled with the conventional resin cement Allcem Core (FGM). Afterwards, the post/cement specimens were cut into discs and subjected to a push-out bond strength (POBS) test. Additionally, 3 posts in each group were evaluated using scanning electron microscopy. The POBS data were analyzed by one-way analysis of variance and the Tukey's honest significant difference post hoc test (${\alpha}=0.05$). Results: The Blasting and $NH_3$ groups showed the highest POBS values. The HMDSO group showed intermediate POBS values, whereas the Control and $H_2O_2$ groups showed the lowest POBS values. Conclusion: Blasting and $NH_3$ plasma treatments were associated with stronger bonding of the conventional resin cement Allcem to fiber posts, in a procedure in which the Ambar adhesive was used.

The use of auxiliary devices during irrigation to increase the cleaning ability of a chelating agent

Prado, Marina Carvalho,Leal, Fernanda,Simao, Renata Antoun,Gusman, Heloisa,do Prado, Maira The Korean Academy of Conservative Dentistry 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: This study investigated the cleaning ability of ultrasonically activated irrigation (UAI) and a novel activation system with reciprocating motion (EC, EasyClean, Easy Equipamentos $Odontol\acute{o}gicos$) when used with a relatively new chelating agent (QMix, Dentsply). In addition, the effect of QMix solution when used for a shorter (1 minute) and a longer application time (3 minutes) was investigated. Materials and Methods: Fifty permanent human teeth were prepared with K3 rotary system and 6% sodium hypochlorite. Samples were randomly assigned to five groups (n = 10) according to the final irrigation protocol: G1, negative control (distilled water); G2, positive control (QMix 1 minute); G3, QMix 1 minute/UAI; G4, QMix 1 minute/EC; G5, QMix 3 minutes. Subsequently the teeth were prepared and three photomicrographs were obtained in each root third of root walls, by scanning electron microscopy. Two blinded and pre-calibrated examiners evaluated the images using a four-category scoring system. Data were statistically analyzed using Kruskal-Wallis and Dunn tests (p < 0.05). Results: There were differences among groups (p < 0.05). UAI showed better cleaning ability than EC (p < 0.05). There were improvements when QMix was used with auxiliary devices in comparison with conventional irrigation (p < 0.05). Conventional irrigation for 3 minutes presented significantly better results than its use for 1 minute (p < 0.05). Conclusions: QMix should be used for 1 minute when it is used with UAI, since this final irrigation protocol showed the best performance and also allowed clinical optimization of this procedure.

The effects of non-thermal plasma and conventional treatments on the bond strength of fiber posts to resin cement

Maíra do Prado,Eduardo Moreira da Silva,Juliana das Neves Marques,Caroline Brum Gonzalez,Renata Antoun Simão 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.2

This study compared the effect of hexamethyldisiloxane (HMDSO) and ammonia (NH3) plasmas on the bond strength of resin cement to fiber posts with conventional treatments. Materials and Methods: Sixty-five fiber posts were divided into 5 groups: Control (no surface treatment); H2O2 (24% hydrogen peroxide for 1 min); Blasting (blasting with aluminum oxide for 30 sec); NH3 (NH3 plasma treatment for 3 min); HMDSO (HMDSO plasma treatment for 15 min). After the treatments, the Ambar adhesive (FGM Dental Products) was applied to the post surface (n = 10). The fiber post was inserted into a silicon matrix that was filled with the conventional resin cement Allcem Core (FGM). Afterwards, the post/cement specimens were cut into discs and subjected to a push-out bond strength (POBS) test. Additionally, 3 posts in each group were evaluated using scanning electron microscopy. The POBS data were analyzed by one-way analysis of variance and the Tukey’s honest significant difference post hoc test (α = 0.05). Results: The Blasting and NH3 groups showed the highest POBS values. The HMDSO group showed intermediate POBS values, whereas the Control and H2O2 groups showed the lowest POBS values. Conclusion: Blasting and NH3 plasma treatments were associated with stronger bonding of the conventional resin cement Allcem to fiber posts, in a procedure in which the Ambar adhesive was used.

The use of auxiliary devices during irrigation to increase the cleaning ability of a chelating agent

Marina Carvalho Prado,Renata Antoun Simão,Fernanda Leal,Heloisa Gusman,Maíra do Prado 대한치과보존학회 2017 Restorative Dentistry & Endodontics Vol.42 No.2

Objectives: This study investigated the cleaning ability of ultrasonically activated irrigation (UAI) and a novel activation system with reciprocating motion (EC, EasyClean, Easy Equipamentos Odontológicos) when used with a relatively new chelating agent (QMix, Dentsply). In addition, the effect of QMix solution when used for a shorter (1 minute) and a longer application time (3 minutes) was investigated. Materials and Methods: Fifty permanent human teeth were prepared with K3 rotary system and 6% sodium hypochlorite. Samples were randomly assigned to five groups (n = 10) according to the final irrigation protocol: G1, negative control (distilled water); G2, positive control (QMix 1 minute); G3, QMix 1 minute/UAI; G4, QMix 1 minute/EC; G5, QMix 3 minutes. Subsequently the teeth were prepared and three photomicrographs were obtained in each root third of root walls, by scanning electron microscopy. Two blinded and pre-calibrated examiners evaluated the images using a four-category scoring system. Data were statistically analyzed using Kruskal-Wallis and Dunn tests (p < 0.05). Results: There were differences among groups (p < 0.05). UAI showed better cleaning ability than EC (p < 0.05). There were improvements when QMix was used with auxiliary devices in comparison with conventional irrigation (p < 0.05). Conventional irrigation for 3 minutes presented significantly better results than its use for 1 minute (p < 0.05). Conclusions: QMix should be used for 1 minute when it is used with UAI, since this final irrigation protocol showed the best performance and also allowed clinical optimization of this procedure.

Chemical Composition and Fatty Acid Profile in Crossbred (Bos taurus vs. Bos indicus) Young Bulls Finished in a Feedlot

Ducatti, Taciana,do Prado, Ivanor Nunes,Rotta, Polyana Pizzi,do Prado, Rodolpho Martin,Perotto, Daniel,Maggioni, Daniele,Visentainer, Jesui Vergilio Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.3

This study was conducted to determine the effect of breed on the chemical composition of first (PUR1) and second (PUR2) generations of $Purun{\tilde{a}}$ young bulls, and to compare both generations with different genetic groups: CAN vs. ANG; CAR; and CHA vs. CAR. Thirty bulls were used. The animals from the PUR2 and CHA vs. CAR genetic groups featured higher (p<0.05) moisture percentage in comparison to the PUR1, CAR and CAN vs. ANG groups. The moisture percentage was similar (p>0.05) between PUR2 and CHA vs. CAR animals. The same was observed among the PUR1, CAR, CAN vs. ANG and CHA vs. CAR animals. There was no difference (p>0.05) among genetic groups for ash, total lipids and total cholesterol. The PUR1, CAR and CAN vs. ANG specimens had higher (p<0.05) crude protein percentages as compared to PUR2 and CAN vs. CAR animals. The fatty acid profile was different (p<0.05) among genetic groups. However, the percentages of SFA, MUFA, PUFA, n-6, n-3, PUFA:SFA, and n-6:n-3 were similar (p>0.05) among genetic groups.