http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Zou Xuexiao (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
A Novel Pepper (Capsicum annuum L.) WRKY Gene, CaWRKY30, Is Involved in Pathogen Stress Responses
Zheng Jingyuan,Zou Xuexiao,Mao Zhenchuan,Xie Bingyan 한국식물학회 2011 Journal of Plant Biology Vol.54 No.5
WRKY proteins are a superfamily of transcription factors involved in many plant processes including plant defense responses to biotic and abiotic stresses. We isolated a WRKY gene from pepper during the incompatible interaction between the pepper cultivar HDA149 and Meloidogyne incognita. The full-length gene, named as CaWRKY30, has a 1,533-bp cDNA sequence and contains an open reading frame of 1,095 bp, encodes a putative polypeptide of 364 amino acids with a theoretical protein size of 41.2 kDa, and contains one WRKY domain followed by a zinc-finger motif. The genomic sequence of CaWRKY30 contains three exons and two introns. Southern blot analysis confirmed that CaWRKY30 exists as a single copy in the pepper cultivar HDA149 genome. Quantitative RT-PCR showed that CaWRKY30 is up-regulated by application of various pathogens including avirulent M. incognita, Tobacco mosaic virus, Ralstonia solanacerum,and Phytophthora capsici Leonian. Furthermore, the transcripts of CaWRKY30 were rapidly induced after treatment with phytohormones salicylic acid (SA). However, the expression of CaWRKY30 was down-regulated by virulent M. incognita and phytohormones methyl jasmonic acid (MeJA). In addition, the nuclear localization of CaWRKY30 was determined when a CaMV35s::CaWRKY30-eGFP fusion construct was expressed in onion epidermal cells. These results suggested that CaWRKY30might be involved in plant defense mechanisms against the diverse pathogen infection.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
