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- 검색키워드
- 저자 : Zhixia Ding (검색결과 3 건)
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Revelation of the early responses of salt tolerance in maize via SSH libraries
Dong Ding,Zhixia Xiao,Hailin Xiao,Tao Xia,YongLian Zheng,Fazhan Qiu 한국유전학회 2012 Genes & Genomics Vol.34 No.3
Maize (Zea mays L.), one of the most important cereal crops in the world, is salt-sensitive. It is critical to study the genes associated with salt tolerance, the differential gene expression pattern between salt-tolerant and salt-sensitive lines, and the mechanism of salt tolerance in maize. Two maize inbred lines with different salt sensitivity, NC286 and Huangzao4, were used to reveal early responsive genes under high salinity condition. Suppression subtractive hybridization (SSH) assay was conducted to identify potential genes involved in the early stage of maize seedlings in responses to 200mM NaCl. A custom μparafloTM microfluidic array (LC science USA) containing Release version 10.1 plant miRNA probes (http://microrna. sanger.ac.uk/) was used to discover salt stress responsive miRNAs using the differences in miRNAs expression between these two maize lines. Cis-elemnet analysis was performed in the promoter regions of these two kinds of salt stress responsive transcriptions. The expression of a total of 141unique genes was significantly changed under 200mM NaCl salt shock within 24 hours. These genes were involved in a broad spectrum of biochemical, cellular, and physiological processes. They were assigned to 14 categories based on their biological functions. Some of them were previously reported to be associated with biotic and abiotic stresses, and several novel genes detected. A gene model was proposed which showed how these genes could sense and response to the high salinity environment. The promoter regions of these 141 salt responsive genes were analyzed, and salt responsive key cis-elements uncovered. The cis-elements usage bias of the salt responsive mRNAs and non-coding RNAs were of the same. The different expression profiles of the genes, especially the ones involved in salt signal cascades and phytohormones responding,could explain the different salt stress response in the two maize inbred lines. The similar cis-elements usage bias of two kinds of salt responsive transcriptions suggests they may be regulated by the same trans-factors.
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Nonlinear Finite Element Modeling of Two-stage Energy Dissipation Device with Low-yield-point Steel
Shenggang Fan,Zhixia Ding,Li Du,Chunfang Shang,Meijing Liu 한국강구조학회 2016 International Journal of Steel Structures Vol.16 No.4
Equipped with many advantages, such as low yield strength, low yield ratio, high elongation, severe plastic deformation and good energy dissipation ability, low-yield-point steel is very suitable for use in metal energy dissipation devices. Based on different materials (Q235 steel and low-yield-point steel) and different parabola openings, two types of energy dissipation steel plates underwent different yield displacements and then were assembled into a new open-hole energy dissipation device, which could achieve the goal of two-stage energy dissipation under small and large earthquakes. To obtain the failure modes and energy dissipation mechanism under the low reversed cyclic horizontal loads and observe relevant hysteretic curves and skeleton curves, the new energy dissipation device was studied and analyzed by means of theoretical analysis, experimental research, and numerical simulation analysis. Based on parametric analyses, the effects of the height, thickness and opening coefficient of the steel plate on the energy dissipation ability of the new energy dissipation device were emphasized. Thus, the key parameters affecting the energy dissipation behavior were obtained. Finally, a force-restoring model of the new energy dissipation device was put forward, and the calculation formulas were given for many parameters, including stiffness, yield displacement, yield load, ultimate displacement and ultimate load. The results show that the new open-hole energy dissipation device has the advantages of superior energy dissipation performance, obvious energy dissipation in two stages, and wide application prospects in structural seismic design.
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Research on Local Buckling of Stainless Steel Lipped C-Section Beam Around Strong-Axis
Shenggang Fan,Hang Zhou,Zhixia Ding,Chenxu Li,Qinglin Jiang 한국강구조학회 2022 International Journal of Steel Structures Vol.22 No.5
To study the local buckling capacity of stainless steel beams with lipped C-sections under strong axis bending, tensile tests were performed on 12 stainless steel coupons based on S30408 austenitic stainless steel (AISI304) in fl at and corner areas, and mechanical properties and stress–strain curves were obtained. Then, local buckling capacity tests were performed on 6 specimens of stainless steel beams under strong axis bending to determine their mechanical properties and failure mechanism. The failure phenomenon, load–displacement curve, load–strain curve and local buckling capacity were determined. Results show that the failure modes of the specimens are local buckling failure of the fl ange and web at mid-span. Additionally, a refi ned fi nite element analysis model was developed using Python and ABAQUS to simulate and analyse the mechanical performance and local buckling capacity. Then, the analytical results were compared to the test results, and the accuracy of the refi ned model was verifi ed. Then, the refi ned model of the stainless steel C-section beam was simplifi ed including the constraint simplifi cation model, which considered diff erent fl ange constraints and initial imperfections, and the length simplifi cation model, which was based on diff erent length and support constraints. The comparative analysis results showed that the constraint simplifi cation model can simulate the local buckling failure mode more accurately than other models. Additionally, initial imperfections were shown to have little eff ect on the local buckling capacity; however, specimen length and the bearing constraint condition did aff ect the local buckling capacity.
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