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Kazuhiko Sakamoto,Tomohiro Sakaguchi,Zhaowu Jiang,Tarek Mohamed Naser,Kazuhiko Sekiguchi,Qingyue Wang,Katsuyuki Takahashi 한국대기환경학회 2007 한국대기환경학회 학술대회논문집 Vol.- No.-
To clarify the effect of the Diesel Vehicle Exhaust Gas Regulations enforced by Tokyo Metropolis, Saitama, Kanagawa, and Chiba Prefectures in October, 2003, we compared concentration of various chemical components in size-separated SPM and traffic volume of 2002 or 2003 (before the Regulation) to that of 2003 or 2004 (after the Regulation) at the road site of National Road Route 17 and Prefecture Road Route 57. Generally, decreasing tendency in EC concentration of each particle sizes was found from 2002 and 2003 to 2003 and 2004 sampling periods, respectively. Especially, decrease in average concentration of EC<SUB><2㎛</SUB> was clear. In the next, we analyzed various correlations of chemical components in size-separated SPM and traffic volume. From their results, we concluded decrease of the EC<SUB><2㎛</SUB> concentration at the roadside was caused by this Regulation. Also, we will discuss on long term trend of mass concentrations and chemical composition for SPM and fine particles observed at general environment and around the other trunk roads in the greater Tokyo Metropolitan Area.
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Zhao Wu,Jinlong Zhao,Wenye Sun,Jing Liang,Jing Jiang 한국분자세포생물학회 2016 Molecules and cells Vol.39 No.9
Transcription activator-like effector nucleases (TALENs) are powerful tools for targeted genome editing in diverse cell types and organisms. However, the highly identical TALE repeat sequences make it challenging to assemble TALEs using conventional cloning approaches, and multiple repeats in one plasmid are easily catalyzed for homologous recombination in bacteria. Although the methods for TALE assembly are constantly improving, these methods are not convenient because of laborious assembly steps or large module libraries, limiting their broad utility. To overcome the barrier of multiple assembly steps, we report a one-step system for the con-venient and flexible assembly of a 180 TALE module library. This study is the first demonstration to ligate 9 mono-/dimer modules and one circular TALEN backbone vector in a one step process, generating 9.5 to 18.5 repeat sequences with an overall assembly rate higher than 50%. This system makes TALEN assembly much simpler than the conventional cloning of two DNA fragments because this strategy combines digestion and ligation into one step using circular vectors and different modules to avoid gel extraction. Therefore, this system provides a convenient tool for the application of TALEN-mediated genome editing in scientific studies and clinical trials.
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