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      • KCI등재

        해외직구사이트의 속성이 중국 소비자의 만족도 및 재구매의도에 미치는 영향

        장영빈(Yongbin Zhang),신건훈(Gun-Hoon Shin) 한국인터넷전자상거래학회 2018 인터넷전자상거래연구 Vol.18 No.1

        The purpose of this study examines the moderating effect of gender relationship between the attributes of overseas direct purchases site and satisfaction. In order to verify the research hypothesis, the empirical analysis was conducted by using SPSS 20.0 and AMOS 18.0. The results of this study are as following. First, the impact of the attributes on satisfaction was partially supported. The effect of product assortment on satisfaction was significant, and hypothesis 1-1 was adopted. The effect of convenience and reliability on customer satisfaction was not significant, but interactivity and customer services have a significant effect on satisfaction, Hypothesis 1-4 and 1-5 were adopted. Secondly, the effect of satisfaction on repurchase intention was significant and Hypothesis 2 was adopted. Thirdly, according to the gender of the demographic attribute, the result of the difference between the attributes of the overseas direct purchase site and the satisfaction was partially adopted. The effect of product assortment and convenience on satisfaction was found to be different in according to the gender, Hypothesis 3-1 and 3-2 were adopted. In addition, there was no difference in the effect of reliability and interactivity on satisfaction according to the gender, and Hypothesis 3-3 and 3-4 were rejected. Finally, the effect of customer service on satisfaction was found to be different in according to the gender and Hypotheses 3-5 was adopted.

      • SCIESCOPUSKCI등재

        Cockroach as a Vector of Blastocystis sp. is Risk for Golden Monkeys in Zoo

        Lei Ma,Yongbin Zhang,Haixia Qiao,Shuai Li,Heqin Wang,Ningning Zhang,Xiao Zhang 대한기생충학열대의학회 2020 The Korean Journal of Parasitology Vol.58 No.5

        Blastocystis sp. is a kind of protozoa living in the intestinal tract of human and animals, which will cause intestinal diseases such as diarrhea, abdominal distension and vomiting. This paper was aimed to understand the infection of Blastocystis sp. In golden monkeys and the transmission path in North China. Thirty-seven feces samples from golden monkeys and 116 cockroach samples from Shijiazhuang Zoo were collected from July to October 2019 for PCR analysis of Blastocystis sp. Genetic diversity analysis was further conducted on the samples with positive PCR results. The results showed that the infection rate was 48.7% (18/37) in golden monkeys and 82.8% (96/116) in cockroaches, respectively. The genetic evolution analysis based on small subunit ribosomal RNA demonstrated that three subtypes (ST) of Blastocystis sp. including ST1, ST2, and ST3 existed in the intestinal tract of golden monkeys, while only ST2 was detected in the intestinal tract of cockroaches. This paper may provide supports for the quarantine and control of Blastocystis sp. for the zoo in Northern China.

      • SCIESCOPUS

        Novel discontinuous control for exponential synchronization of memristive recurrent neural networks with heterogeneous time-varying delays

        Zhang, Ruimei,Zeng, Deqiang,Park, Ju H.,Zhong, Shouming,Yu, Yongbin Elsevier 2018 Journal of the Franklin Institute Vol.355 No.5

        <P><B>Abstract</B></P> <P>This paper investigates the exponential synchronization problem of memristive recurrent neural networks (MRNNs) with heterogeneous time-varying delays (HTVDs). First, a novel discontinuous feedback control is designed, in which a tunable scalar is introduced. The tunable scalar makes the controller more flexible in reducing the upper bound of the control gain. Based on this control scheme, the double integral term can be successfully used to construct the LKF. Second, New method for tackling memristive synaptic weights and new estimation technique are presented. Third, based on the LKF and estimation technique, synchronization criterion is derived. In comparison with existing results, the established criterion is less conservatism thanks to the double integral term of the LKF. Finally, numerical simulations are presented to validate the effectiveness and advantages of the proposed results.</P>

      • Chemical Composition and Cold Flow Property of Cottonseed Oil Biodiesel Fuel

        Yongbin Lai,Junfeng Shu,Xiu Chen,Yinnan Yuan,Lei Zhong,Yuqi Zhang,Menghong Yuan,Bo Wang,Pen Wang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.2

        The Cottonseed oil methyl ester (COME) was prepared using an alkali - catalyzed trans esterification reaction, and its chemical composition and cold flow properties (CFP) were studied. Two approaches, viz. blending with petrodiesel and treating with cold flow improver (CFI) were used to improve the CFP of COME. The results showed that COME was mainly composed of fatty acid methyl esters (FAMEs): C14:0–C22:0, C16:1–C20:1, C18:2 and C18:3–C20:3. The mass fractions of saturated and unsaturated FAMEs were 27.69% and 71.65%, respectively. The cold filter plugging point (CFPP) and kinematic viscosity (KV) at 40 °C of COME are -1 °C and 4.63 mm2/s respectively. Blending with -10 petrodiesel (-10PD) and 0 petrodiesel (0PD) can decrease the CFPP of COME/-10PD (B30-B40) and COME/0PD (B40-B50) -12 and -8 °C respectively. With the increase in petrodiesel content, the KV at 40 °C of blending oil decreased, and viscosity-temperature characteristics of blending oil were improved. When used Flow Fit (≤3vol.%), the CFPPs were reduce from COME/- 10PD B5, B7, B10, B20, B50 and COME -8, -8, -9, -11, -11 and -1 °C to -28, -27, -26, -25, -16 and -5 °C respectively; COME/0PD -3, -3, -4, -5, -8 and -1 °C to - 26, -25, -24, -24, -16 and -5 °C respectively.

      • Elite Particle Swarm Optimization Algorithm for Solving the Bi-Criteria No-wait Flexible Flow Shop Problem

        Yongbin Qin,Haiyue Zhang 보안공학연구지원센터 2016 International Journal of Grid and Distributed Comp Vol.9 No.12

        The thesis mainly studies bi-criteria no-wait flexible flow shop problem, whose optimi-zation objective is to minimize the maximum completion time and the maximum delay time. This problem is NP hard, yet enjoying important theoretical research value, thereby this thesis proposes elite particle swarm optimization (EPSO) to solve bi-criteria no-wait flex-ible flow shop problem. EPSO algorithm applies five modified heuristic algorithms and random methods to generating initial population. Moreover, for the particle personal best, this thesis puts forward elite crossover algorithm, which retains continuous fragments of the identical workpieces among excellent individuals, avoiding the destruction of good continuity between solutions of workpieces. In addition, in order to avoid algorithm into local optimum, this thesis raises double insertion disturbance algorithm to help particles jump out the local optimal state and expand the feasible search range. For the purpose of effectively evaluating algorithm quality, there is a comparison among EPSO algorithm, PSO algorithm and ICA algorithm in simulation experiment that is respectively aimed at small-scale problem and large-scale scheduling problem, the results of which show that the proposed EPSO algorithm, due to better validity and accuracy, is superior to the PSO algorithm and ICA algorithm.

      • Study on Volatility of Palm Oil Biodiesel/-10 Petrodiesel by Thermogravimetric Analysis Technique

        Yongbin Lai,Peng Wang,Xiu Chen,Yinnan Yuan,Junfen Rong,Guang Wu,Yijun Zhou,Lei Zhong,Yuqi Zhang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.2

        Palm methyl ester (PME) was prepared from palm oil through transesterification using NaOH as catalyst. Chemical composition of the PME and -10 petrodiesel (-10PD) was determined by gas chromatography-mass spectrometer (GC-MS). The PME and -10PD were characterized for their fuel properties including density, kinematic viscosity, and flash point, cold filter plugging point, sulfur content, copper strip corrosion and oxidative stability. Volatility was measured by thermogravimetric analysis (TGA). Volatile index was proposed to describe PME/-10PD volatility. A good correlation model was put forward for calculating the PME/-10PD volatility by PME blending ratio. The study showed that PME was mainly composed of long chain fatty acid methyl esters (FAMEs): C14:0-C24:0, C16:1-C22:1, C18:2 and C18:3. -10PD was mainly composed of long chain alkanes: C8-C26. The fuel properties of PME were within the limits prescribed in the GB/T 20828-2007 standards for biodiesel. With respect to -10PD, volatilization of PME was stronger and quicker, but volatilization onset at higher temperature. The volatilization onset temperatures of PME and -10PD were 448.9 and 361.7 K respectively; and the volatile indexes were 1.76E-04 and 3.64E-05 respectively. The PME/-10PD volatility had relation to PME blending ratio. The volatility of B0-B20 was very close to the -10PD. The volatility of B20-B100 was better with increasing the PME blending ratio.

      • KCI등재

        Structural and biochemical analysis of Glyceraldehyde-3-Phosphate Dehydrogenase from Clostridium beijerinckii

        Jie Zhang,Tingting Bu,Yuanyuan Chen,Xue Bai,Shanru He,Yongbin Xu 한국구조생물학회 2022 Biodesign Vol.10 No.4

        Clostridium beijerinckii is a promising industrial microorganism for its ability to produce butanol, acetone, and isopropanol using a wide range of substrates, including pentoses, hexoses, and starch, via fermentation. The ubiquitous and highly abundant glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is essential for most organisms’ energy and carbon metabolism, which plays a critical role in some industrial bacteria. It catalyzes the simultaneous oxidation and phosphorylation of D-glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD+). We determined the crystal structure of the GAPDH from C. beijerinckii (C. beijerinckii GAPDH). C. beijerinckii GAPDH consists of an α-β-α domain which shares an evolutionarily conserved fold consisting of two juxtaposed domains, an N-terminal NAD+-binding domain (NBD) and a C-terminal catalytic domain (CD). These findings provide insight into the molecular mechanism of action and cofactorbinding of this important industrial bacterial enzyme.

      • KCI등재후보

        Clostridium beijerinckii glyceraldehyde-3-phosphate dehydrogenase GAPDH: purification, crystallization, and X-ray crystallographic analysis

        Jie Zhang,Yuanyuan Chen,Tingting Bu,Xue Bai,Shanru He,Lulu Wang,Chunshan Quan,Yongbin Xu 한국구조생물학회 2022 Biodesign Vol.10 No.3

        Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous and highly abundant glycolytic enzyme. It plays a pivotal role for the energy and carbon metabolism of most organisms including industrial bacteria. It catalyzes the two step oxidative phosphorylation of D-glyceraldehyde-3-phosphate into 1,3-bisphosphoglycerate using inorganic phosphate and nicotinamide adenine dinucleotide (NAD+) as cofactor. In this study, GAPDH from C. beijerinckii (CbGAPDH) was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange, and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 1.60 Å. The crystal belonged to the hexagonal space group P6222, with unit-cell parameters of a = 120.6, b = 120.6, and c = 122.1 Å. The Matthews coefficient and solvent content were estimated to be 3.50 Å3 Da–1 and 64.90%, respectively, assuming that the asymmetric unit contained only one recombinant protein molecule.

      • KCI등재

        Candida auris Aldo-keto reductase AldO: purification, crystallization, and X-ray crystallographic analysis

        Jie Zhang,Xue Bai,Shanru He,Yongbin Xu 한국구조생물학회 2023 Biodesign Vol.11 No.3

        Candida auris is a new fungus that poses a serious global health threat due to its resistance to multiple antifungal medications, making treatment challenging. Aldo-keto reductase (AKR) represents a versatile superfamily of enzymes that play pivotal roles in the phase I metabolism of various metabolic and detoxification processes. In this study, AKR from C. auris (CaAldO) was successfully expressed and purified using Ni-NTA affinity, Q anion exchange, and gel-filtration chromatography. The protein crystal was obtained and diffracted to a resolution of 1.95 Å. The crystal belonged to the orthorhombic space group P212121, with unit-cell parameters of a = 71.65, b = 91.15, and c = 227.66 Å. The Matthews coefficient and solvent content were estimated to be 2.32 Å3 Da–1 and 47.00%, respectively, assuming the asymmetric unit contained four recombinant protein molecules.

      • KCI등재

        Self-nitrogen-doped carbon materials derived from the petioles and blades of apricot leaves as metal-free catalysts for selective oxidation of aromatic alkanes

        Sun Yongbin,Hao Junlei,Zhu Xuesai,Zhang Baobin,Yin Hao,Xu Shanguang,Hou Chao,Liu Kun 한국탄소학회 2020 Carbon Letters Vol.30 No.2

        Carbon materials with tailorable structures and superior properties have great potential applications in environmental protec�tion, energy conversion, and catalysis. Plant biomass as abundant and green non-toxic raw materials has been considered as good precursors for synthesizing heteroatom-doped carbon materials. However, few studies have been reported on the dif�ferent natures of carbon materials derived from diferent parts of the same plant biomass. In this study, we prepared carbon materials from the petioles and blades of apricot leaves by direct pyrolysis without additives. Detailed characterizations indicate that these two carbon materials are similar in element composition and graphitization degree, but difer greatly in surface area and pore volume. These diferences can be attributed to the diferent contents of inorganic salts, vascular bundles, and proteins in petioles and blades. When used as catalysts for the oxidation of ethylbenzene, the petiole-derived carbon shows better catalytic performance than the blades derived carbon due to its high surface area, large average pore size, and doped nitrogen atoms. Furthermore, the carbon catalysts derived from the petioles and blades of poplar leaves and parasol tree leaves show the same diference in catalytic reaction, implying that the above-mentioned conclusion is rather universal, which can provide reference for the synthesis of carbon materials from leaves.

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