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RISS 인기검색어
- 검색키워드
- 저자 : Zabihollah Zamani (검색결과 10 건)
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Abdolkarim Zarei,Zabihollah Zamani,Mohammad Reza Fatahi Moghadam 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.3
Twenty-eight cultivated pomegranates, one hybrid seedling and one wild accession from three provinces (five eco-geographical regions) of Iran were used for the determination of their genetic relationships by using RAPD markers. Sixty-seven RAPD decamer primers were screened, out of which twenty were amplified good, reproducible and polymorphic fragments among accessions. From a total of 309 recorded fragments, 174 were polymorphic between accessions (57.64%). The Jaccard’s similarity coefficients ranged from 0.15 (between the wild accession and Poost-Sefide-Torsh cv.) to 0.95 (between ‘Shirin-Shahvar’ and ‘Shirine-Hastei’). The collective resolving power (Rp) rate value of 36.08 was scored for all RAPD primer bands. UPGMA dendrogram clustered the accessions in agreement with their geographical origins. In addition, nine SSRs loci were examined on these accessions that only one of them was polymorphic. This study revealed that the wild accession of pomegranate was genetically different from domesticated ones, indicating that wild genotypes are worthy for consideration in breeding programs of pomegranate.
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Assessment of Morphological Variation between Some Iranian Wild Cerasus Sub-genus Genotypes
Ali Shahi-Gharahlar,Zabihollah Zamani,Mohammad Reza Fatahi,Naser Bouzari 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.4
Iran is one of the important countries for the distribution of Cerasus subgenus species with around 225,000 tons production of cherries. Together with Turkey and USA it has been one among the three leading producers of these fruits. One of the important aims in cherry breeding is developing dwarfing rootstocks with good adaptation with different climates and resistance to biotic and abiotic stresses. A program has been initialized in Iran for breeding and developing new rootstocks for cherries, suitable for climatic and edaphic condition of this country. The present study was conducted under this program to categorize some Cerasus sub-genus wild genotypes and improved cultivars and rootstocks, by using vegetative characters. Conservation of the genetic resources of these species is another important aim of this program. Around 27 morphological parameters were evaluated on 74 genotypes in seven species of this sub-genus from eight provinces of Iran. These consisted of P. avium L., P. cerasus L., P. mahaleb L., P. incana Pall., P. brachypetala Boiss., P. pseudoprostrata Pojark. and P. microcarpa Bioss. Analysis of variance of the studied parameters was significant among genotypes, showing a high level of variability for the characters. Results of simple correlation analysis showed the existence of significant positive and negative correlations among some important parameters such as tree height, tree growth habit, trunk diameter, internode length, growth compactness and leaf area. Also, factor analysis showed that tree growth habit, height, growth compactness, trunk diameter, internode length, pubescence on the leaf upper surface and number of leaf serration per cm constitute the main factors. Effective characters were categorized within six factors and accounted for 77.74 of overall variance. Cluster analysis was performed using these six factors and genotypes in the approximate distance of 13 were divided into six main groups. These groups were divided into several sub-clusters and improved varieties or cultivars were clearly separated from the others. The P. cerasus cultivars and genotypes together with one genotype of the P. avium and improved rootstocks (‘Gisela6’ and ‘Colt’) located in one sub-cluster. Scatter plot analysis using two main factors could also clearly separate the location of genotypes and verified genotypes from each other.
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Reza Fatahi,Aziz Ebrahimi,Zabihollah Zamani 한국원예학회 2010 Horticulture, Environment, and Biotechnology Vol.51 No.1
Walnut, (Juglans regia), is one of the most important nutritive nut crops. Iran is considered as one of the centers for diversity and cultivation of walnut in middle-eastern part of the world. In this study, some morphological traits in combination with polymorphic RAPD primers were applied to evaluate genetic potential of 35 (31 Iranian and four foreign) walnut genotypes. From 14 RAPD primers, 180 DNA bands were amplified among which 174 were highly polymorphic. The lowest and the highest pair-wise similarity coefficients between examined genotypes were 0.27 and 0.89, respectively. Estimated resolving power index (Rp) were from 2.17 (opAD16) to 8.40 (opAA19) and total resolving power (Rp) 70.61 in all 14 RAPD loci. Cluster analysis based on Jaccard's similarity coefficients and UPGMA method divided the genotypes into five groups at similarity of 0.50, which the main group consisted of 31 genotypes including 4 foreign cultivars. Genetic variation among locations took 9.48% of the total genetic variation detected with Analysis of molecular variances (AMOVA) (p<0.001). Most Iranian genotypes grouped closely with foreign cultivars and showed high similarities indicating their close genetic relatedness. RAPD markers showed to be an efficient technique for studying genetic diversity of walnut genotypes. These genotypes with different genetic bases and suitable horticultural traits seem to be good candidates applicable for direct use as vegetatively propagated material or as parents in walnut improvement programs.
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Abdollah Khadivi-Khub,Zabihollah Zamani,Naser Bouzari 한국원예학회 2008 Horticulture, Environment, and Biotechnology Vol.49 No.3
Sweet cherry (Prunus avium L.), a member of Rosaceae family, is an economically important fruit of the temperate zone. In Iran, various sweet cherry genotypes are grown in different areas. For estimation of genetic diversity, 23 RAPD decamer primers data as well as 23 morphological traits were used on 39 sweet cherry cultivars and genotypes, 28 of Iranian and 11 of foreign origin. Random Amplified Polymorphic DNA (RAPD) data was used for clustering of genotypes using UPGMA method. Based on the results, in some cases, clustering of genotypes by RAPD data was in agreement with morphological data; however, the correlation between the two sets of data was not significant (r = 0.2). The coephenitic coefficients between genotypes varied from 0.43 to 0.83 and the value of calculated polymorphism was 81.7 percent, indicating the presence of a high variation between the studied cultivars. This could be due to the presence of both Iranian and foreign genotypes in the experiment. In the main subcluster, genotypes from both origins were present and some genotypes were showing close relationships. Significant regression associations were found between 7 morphological traits and RAPD markers and some informative markers were found for the traits. Also, in clustering of genotypes good agreements were found between the subclusters and the pollination incompatibility groups reported by other workers. The results showed that RAPD is an effective maker for study of genetic diversity among sweet cherry genotypes.
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Evaluation of Genetic Diversity among Some Genotypes of Kentucky Bluegrass by RAPD Molecular Markers
Javad Rezapour Fard,Zabihollah Zamani,Mohamad Reza Fattahi Moghaddam,Mohsen Kafi 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.4
Genetic improvement of plants need a high genetic diversity and molecular markers offer a powerful tool for its assessment. Poa pratensis has extensive divergence in natural old turfs and pastures in temperate zones of Iran,being useful for breeding programs. In this research, RAPD markers were used to study the genetic diversity of 19turfgrass genotypes (including 16 genotypes of Poa pratensis along with one genotype from each of Lolium perenne,Cynodon dactylon and Festuca arundinacea species). After screening 100 random 10-mer RAPD primers, 17 primers were selected based on the clarity of their amplified fragments in PCR and the high reproducibility for scoring. From the 284 produced bands, 281 were polymorphic. Cluster analysis of RAPD data was performed using the UPGMA method based on Jaccard's similarity coefficients. Dendrogram at a similarity of 0.24 gave 5 main clusters. The correlation coefficient between the data matrix and the cophenetic matrix of cluster data was high (r = 0.99), indicating that the clustering dendrogram being highly fitted to similarity matrix. A relatively high genetic similarity was observed between some commercial cultivars and Iranian local genotypes. In addition, this research showed the high genetic diversity among studied genotypes as well as the high efficiency of RAPD markers for evaluation of genetic diversity among turfgrasses.
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Mohammad Fattahi,Vahideh Nazeri,Fatemeh Sefidkon,Zabihollah Zamani,Javier Palazon 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.6
The aim of this study was to investigate the effect of pre-sowing treatments and light on Dracocephalum kotschyi Bioss. seed germination. The following treatments were used: sandpaper; 3, 5 and 10 min soaking in sulfuric acid; 15 min in sodium hypochlorite; 24 h in water, hot water and boiling water; 50, 150, and 250 mg・L^(-1) of Gibberellic acid; 1000, 2000,4000, and 6000 mg・L^(-1) of KNO3, and water (control). The germination rate, germination start (GS), mean germination time (MGT), time to 50% germination (T_(50)) and cumulative germination were determined as germination indices. Root, shoot and seedling length and the vigor index (VI) were evaluated as growth factors at the end of the incubation period. Second experiment was carried out to study the effect of light on seed germination. It was observed that both the physical and chemical scarification methods were effective in enhancing D. Kotschyi seed germination and growth. In the first stage, up to day 20 SA (10 min) and sandpaper scarification treated seed had shown germination more than 70% whereas control seeds did not begin germination until day 20. The highest germination rate was observed with sandpaper (1.77 units) and SA (1.56 units)in compare with other treatments (less than 0.9 units). Also the highest vigor index was found in SA- and sandpaper-treated seeds and the lowest occurred in sodium hypochlorite treatments. The other results showed that light was more effective than darkness for seed germination.
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Raheleh Ebrahimi,Mohammad Reza Hassandokht,Zabihollah Zamani,Abdolkarim Kashi,Isabel Roldan-Ruiz,Erik Van Bockstaele 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.1
This study was conducted to evaluate seed morphogenesis and effect of pretreatments on seed germinationof Persian shallot or mooseer (Allium hirtifolium Boiss.). In a first experiment, seed developmental stages of Persianshallot (Mooseer) were assessed. Results showed that number of ovules varied among locules and flowers. In mostcases 3-4 ovules occurred per locule and developed into 1-2 seeds and therefore, a ripe capsule usually contained3-6 seeds. The seeds were black in color and ranged from 3.73-3.98 mm in length and 2.75-3.16 mm in width, andthe average weight of 1,000 seeds varied between 7.80-8.20 grams. In a second experiment four factors, scarification,stratification, potassium nitrate, and GA3, were evaluated for seed germination. Results showed that seed germinationwas not observed without sandpaper scarification or stratification treatment. It could be concluded that the best treatmentfor overcoming mooseer seed dormancy and getting the longest seedling length was scarification with sandpaper, GA3(500 mg·L-1 for 12 hours) application, and moist stratification. The results obtained in the present study revealed thatmooseer seed has mechanical dormancy and only cold stratification is needed for endosperm weakening.
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