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Poly(L-Ala-co-L-Lys) Exhibits Excellent Ice Recrystallization Inhibition Activity
Zhengyu Piao,박진경,백지현,정병문 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.2
The control of ice recrystallization is very important in cryo-technological fields. Ice recrystallization inhibition (IRI) compounds are therefore designed to limit the growth of ice crystals and control the crystal shape. To improve the IRI activity of cryo-systems, various synthetic polymers such as biomimetic polypeptides from polar fish, facially amphiphilic polymers, polyampholytes, poly(vinyl alcohol) derivatives, and block copolymers with hydrophilic-hydrophobic balance have been developed. Herein, poly(L-alanine-co-L-lysine) (PAK) was shown to exhibit a similar IRI activity to that of PVA. Furthermore, neither poly(L-alanine-co-L-aspartic acid) (PAD) nor poly(ethylene glycol) (PEG) with a similar molecular weight provided any significant IRI activity. These results suggest that PAK is an important compound with superior IRI activity, and that this activity is dependent upon the functional groups and secondary structure of the polypeptides.
Block Copolymer as a Cryoprotectant
박진경,정병문,모두미타파텔,Zhengyu Piao 한국고분자학회 2021 한국고분자학회 학술대회 연구논문 초록집 Vol.46 No.1
Understanding of the ice crystallization process and cell membrane kinetics during cryopreservation plays a key role in storing cells. Here we synthesized poly(ethylene glycol)-poly(L-alanine) block copolymers with various block length. Depending on the block length, size of ice crystals varied, and the shape was also diverse from spherical to rod-like sharp shape depending on the concentration and the kind of polymers. Stem cell recovery improved as the size of ice crystal decreased, however it dropped as the ice crystal shape was rod-like shape. Cell recovery also seems to be affected by cell membrane stabilization through the polymer. The recovered cell exhibited excellent proliferation and underwent into osteo-, chondro-, and adipogenic differentiation. Contrary to dimethysulfoxide (DMSO), current cryoprotectant, PEG-PA proved to be cytocompatible. The paper suggests that P1 among PEG-L-PAs can be an excellent cryoprotectant for stem cells to replace cytotoxic DMSO.