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RISS 인기검색어
- 검색키워드
- 저자 : Yukako Yagi (검색결과 4 건)
- 무료
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Basic Physiological Research on the Wing Flapping of the Sweet Potato Hawkmoth Using Multimedia
Nakajima, Isao,Yagi, Yukako Korea Multimedia Society 2020 The journal of multimedia information system Vol.7 No.2
We have developed a device for recording biological data by inserting three electrodes and a needle with an angular velocity sensor into the moth for the purpose of measuring the electromyogram of the flapping and the corresponding lift force. With this measurement, it is possible to evaluate the moth-physiological function of moths, and the amount of pesticides that insects are exposed to (currently LD50-based standards), especially the amount of chronic low-concentration exposure, can be reduced the dose. We measured and recorded 2-channel electromyography (EMG) and angular velocity corresponding to pitch angle (pitch-like angle) associated with wing flapping for 100 sweet potato hawkmoths (50 females and 50 males) with the animals suspended and constrained in air. Overall, the angular velocity and amplitude of EMG signals demonstrated high correlation, with a correlation coefficient of R = 0.792. In contrast, the results of analysis performed on the peak-to-peak (PP) EMG intervals, which correspond to the RR intervals of ECG signals, indicated a correlation between ΔF fluctuation and angular velocity of R = 0.379. Thus, the accuracy of the regression curve was relatively poor. Using a DC amplification circuit without capacitive coupling as the EMG amplification circuit, we confirmed that the baseline changes at the gear change point of wing flapping. The following formula gives the lift provided by the wing: angular velocity × thoracic weight - air resistance - (eddy resistance due to turbulence). In future studies, we plan to attach a micro radio transmitter to the moths to gather data on potential energy, kinetic energy, and displacement during free flight for analysis. Such physiological functional evaluations of moths may alleviate damage to insect health due to repeated exposure to multiple agrochemicals and may lead to significant changes in the toxicity standards, which are currently based on LD50 values.
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Autocorrelation Coefficient for Detecting the Frequency of Bio-Telemetry
Yoshiya Muraki,Yukako Yagi,Kiyoshi Kurokawa,Isao Nakajima 한국멀티미디어학회 2022 The journal of multimedia information system Vol.9 No.3
A MATLAB program was developed to calculate the half-wavelength of a sine-curve baseband signal with white noise by using an autocorrelation function, a SG filter, and zero-crossing detection. The frequency of the input signal can be estimated from 1) the first zero-crossing (corresponding to ¼λ) and 2) the R value (the Y axis of the correlogram) at the center of the segment. Thereby, the frequency information of the preceding segment can be obtained. If the segment size were optimized, and a portion with a large zero-crossing dynamic range were obtained, the frequency discrimination ability would improve. Furthermore, if the values of the correlogram for each frequency prepared on the CPU side were prepared in a table, the volume of calculations can be reduced by 98%. As background, period detection by autocorrelation coefficients requires an integer multiple of 1/2λ (when using a sine wave as the object of the autocorrelation function), otherwise the correlogram drawn by R value will not exhibit orthogonality. Therefore, it has not been used in bio-telemetry where the frequencies move around.
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Technical Evaluation of Engineering Model of Ultra Small Transmitter Mounted on Sweetpotato Hornworm
Isao Nakajima,Yoshiya Muraki,Kokuryo Mitsuhashi,Hiroshi Juzoji,Yukako Yagi 한국멀티미디어학회 2022 The journal of multimedia information system Vol.9 No.2
The authors are making a prototype flexible board of a radio-frequency transmitter for measuring an electromyogram (EMG) of a flying moth and plan to apply for an experimental station license from the Ministry of Internal Affairs and Communications of Japan in the summer of 2022. The goal is to create a continuous low-dose exposure standard that incorporates scientific and physiological functional assessments to replace the current standard based on lethal dose 50. This paper describes the technical evaluation of the hardware. The signal of a bipolar EMG electrode is amplified by an operational amplifier. This potential is added to a voltage-controlled crystal oscillator (27 MHz, bandwidth: 4 kHz), frequency-converted, and transmitted from an antenna about 10 cm long (diameter: 0.03 mm). The power source is a 1.55-V wristwatch battery that has a total weight of about 0.3 g (one dry battery and analog circuit) and an expected operating time of 20 minutes. The output power is −7 dBm and the effective isotropic radiated power is −40 dBm. The signal is received by a dual-whip antenna (2.15 dBi) at a distance of about 100 m from the moth. The link margin of the communication circuit is above 30 dB within 100 m. The concepts of this hardware and the measurement data are presented in this paper. This will be the first biological data transmission from a moth with an official license. In future, this telemetry system will improve the detection of physiological abnormalities of moths.
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Frankenstein Ziv,Naohiro Uraoka,Umut Aypar,Ruth Aryeequaye,Mamta Rao,Meera Hameed,Yanming Zhang,Yukako Yagi 한국현미경학회 2021 Applied microscopy Vol.51 No.1
Fluorescence in situ hybridization (FISH) is a technique to visualize specific DNA/RNA sequences within the cell nuclei and provide the presence, location and structural integrity of genes on chromosomes. A confocal Whole Slide Imaging (WSI) scanner technology has superior depth resolution compared to wide-field fluorescence imaging. Confocal WSI has the ability to perform serial optical sections with specimen imaging, which is critical for 3D tissue reconstruction for volumetric spatial analysis. The standard clinical manual scoring for FISH is labor-intensive, time-consuming and subjective. Application of multi-gene FISH analysis alongside 3D imaging, significantly increase the level of complexity required for an accurate 3D analysis. Therefore, the purpose of this study is to establish automated 3D FISH scoring for z-stack images from confocal WSI scanner. The algorithm and the application we developed, SHIMARIS PAFQ, successfully employs 3D calculations for clear individual cell nuclei segmentation, gene signals detection and distribution of break-apart probes signal patterns, including standard break-apart, and variant patterns due to truncation, and deletion, etc. The analysis was accurate and precise when compared with ground truth clinical manual counting and scoring reported in ten lymphoma and solid tumors cases. The algorithm and the application we developed, SHIMARIS PAFQ, is objective and more efficient than the conventional procedure. It enables the automated counting of more nuclei, precisely detecting additional abnormal signal variations in nuclei patterns and analyzes gigabyte multi-layer stacking imaging data of tissue samples from patients. Currently, we are developing a deep learning algorithm for automated tumor area detection to be integrated with SHIMARIS PAFQ.
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