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- 저자 : Young-ChoonLee (검색결과 2 건)
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Sun-YoungAn,Sang-WanKim,Yong-LarkChoi,Young-SuCho,Woo-HongJoo,Young-ChoonLee 한국미생물학회 2003 The journal of microbiology Vol.41 No.2
The lipase gene (lipA) and its activator gene (lipB) of Pseudomonas sp. SW-3 were cloned and sequenced. The lipB was found to be present immediately downstream of lipA. The deduced amino acid sequences of lipA and lipB showed a high level of homology to those of other lipases belonging to the family I.1 of bacterial lipases. When lipA was expressed in Escherichia coli using T7 promoter, an active lipase was produced in cells carrying both lipA and lipB, but not in cells harboring only lipA. Recombinant lipase (rPSL) overproduced in an insoluble form was solubilized in the presence of 8 M urea, purified in a urea-denatured form and refolded by removing urea in the presence of the Ca2+ ion. rPLS had maximum activity at pH 8.0 and 50oC, was stable at pHs from 7.0 to 9.0 and below 50oC, and showed the highest activity toward the p-nitrophenyl ester of palmitate (C16).
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홍경원,김희수,Joo-MiYi,Kyung-MiShin,Tae-HyungKim,Jae-WonHuh,Young-ChoonLee,Won-HoLee,TimothyJ.Crow 한국유전학회 2003 Genes & Genomics Vol.25 No.2
A human endogenous retroviral family (HERV-W) has been related to multiple sclerosis virus (SZRV) sequences. The HERV-W family was identified in the cerebrospinal fluids and brains of individuals with schizophrenia. Using cDNA libraries derived from human brains, we performed PCR amplification and identified new 16 HERV-W env elements (9 from human adult brain and 7 from human fetal brain). Those sequences showed a high degre of sequence similarity (89.2-99.6%) with HERV-W env (GenBank acesion no. AF072506). Clones HB-1, HB-9 from adult brain and FB-2, FB-5 from fetal brain showed no frameshift and termination codons by deletion/insertion or point mutation. Synonymous and nonsynonymous calculation indicated that these sequences (HB-1, HB-9, FB-2, FB-5) could be asociated with an active provirus in human brain tisues. In phylogenetic analysis, clones HB-1, HB-9, FB-2, FB-5 containing putative amino acid sequences showed sister relationship with the HERV-W and W-7-1 derived from human chromosome 7. Taken together, our data suggest that the related genes of the HERV-W env sequences are expresed in human brains and may contribute to an understanding of biological function connected to neuropsychiatric diseases.
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