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RISS 인기검색어
- 검색키워드
- 저자 : Younes Ghasemi (검색결과 5 건)
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Impact of Amino-Acid Coating on the Synthesis and Characteristics of Iron-Oxide Nanoparticles (IONs)
Ebrahiminezhad, Alireza,Ghasemi, Younes,Rasoul-Amini, Sara,Barar, Jaleh,Davaran, Soodabeh Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.12
Iron-oxide nanoparticles (IONs) with biocompatible coatings are the only nanostructural materials which have been approved by the FDA for clinical use. Common biocompatible coatings such as hydrocarbons, polymers, and silica have profound influences on critical characteristics of IONs. Recently, amino acids were introduced as a novel biocompatible coating. In the present study, the effects of amino acids on IONs synthesis and characteristics have been evaluated. Magnetite nanoparticles with L-arginine and L-lysine coatings were synthesised by a coprecipitation reaction in aqueous solvent and their characteristics were compared with naked magnetite nanoparticles. The results showed that amino acids can be a perfect coating for IONs and would increase particle stability without any significant effects on the critical properties of nanoparticles such as particle size and magnetization saturation value.
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Impact of Amino-Acid Coating on the Synthesis and Characteristics of Iron-Oxide Nanoparticles (IONs)
Alireza Ebrahiminezhad,Younes Ghasemi,Sara Rasoul-Amini,Jaleh Barar,Soodabeh Davaran 대한화학회 2012 Bulletin of the Korean Chemical Society Vol.33 No.12
Iron-oxide nanoparticles (IONs) with biocompatible coatings are the only nanostructural materials which have been approved by the FDA for clinical use. Common biocompatible coatings such as hydrocarbons, polymers, and silica have profound influences on critical characteristics of IONs. Recently, amino acids were introduced as a novel biocompatible coating. In the present study, the effects of amino acids on IONs synthesis and characteristics have been evaluated. Magnetite nanoparticles with L-arginine and L-lysine coatings were synthesised by a coprecipitation reaction in aqueous solvent and their characteristics were compared with naked magnetite nanoparticles. The results showed that amino acids can be a perfect coating for IONs and would increase particle stability without any significant effects on the critical properties of nanoparticles such as particle size and magnetization saturation value.
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Azadeh Mohammadi,Ali Niazi,Farzaneh Aram,Farshid Hassani,Younes Ghasemi 한국작물학회 2020 Journal of crop science and biotechnology Vol.23 No.1
Nowadays, transgenic plants are considered as an expression system for therapeutic recombinant proteins. One of the approaches to recombinant protein production is the introduction of the relevant genes to plant cells and produce hairy roots from these cells using the plant tissue culture methods. L-asparaginase II is one of the important therapeutic enzymes used in the treatment of cancer, especially in the treatment of acute lymphoblastic leukemia (ALL) in children. In this study, L-asparaginase II (ansB) gene was isolated from Escherichia coli YG001 and overexpressed in Solanum tuberosum. The sequence of L-asparaginase II gene was optimized according to the codon usage table of S. tuberosum and cloned into the plant expression vector pBI121. Protein, DNA, and RNA of transgenic hairy roots were investigated. It was demonstrated that the ansB gene was correctly integrated into the genomic DNA of transgenic plants. The whole protein of transgenic roots was used for the assay of L-asparaginase II activity, the higher activity of L-asparaginase II was observed in transgenic hairy roots when compared with non-transgenic ones, and the recombinant protein was detected using western blot.
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Haniye Najafi,Ali Mohammad Tamaddon,Samira Sadat Abolmaali,Bahareh Owrangi,Younes Ghasemi 한국고분자학회 2015 Macromolecular Research Vol.23 No.7
To improve transfection of plasmid DNA as well as serum protein stability of polyionic complex nanoparticles of branched polyethyleneimine (bPEI), poly(ethylene glycol) (PEG)-stabilized nanoparticles were made from L-histidine substituted bPEI (PEI-Histidine) synthesized by Fmoc chemistry. The polymer was characterized by TNBS assay, 1H NMR, GFC, potentiometric titration and elemental analysis of carbon and nitrogen, DNA condensation, and the stability against extracellular matrix (heparin sulfate) was investigated by dye exclusion and agarose gel retardation assays. The nanoparticles were characterized by dynamic light scattering-zeta potential analyzer. Cytotoxicity and expression of enhanced green fluorescent protein (EGFP) were determined in hepatocellular carcinoma by MTT assay and fluorescent techniques. PEI-Histidine showed a reduced pKa without any significant loss of total primary amines. Plasmid DNA was condensed almost thoroughly with PEGylated polymers, either bPEI or PEI-Histidine, at lower critical N/P ratios. PEGylated PEI-Histidine showed the better resistance to heparin induced displacement and the lower cytotoxicity when it was compared to bPEI. Interestingly unlike bPEI, smaller and less positively charged nanoparticles were obtained from PEGylated PEI-Histidine at N/P ratio=2 that resulted in about 4 folds higher EGFP expression than bPEI without any significant cytotoxicity. These properties are consistent with the higher serum protein resistance and buffer capacity of PEGylated PEI-Histidine at endosomal acidic pH.
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Seyyed Vahid Niknezhad,Mohammad Hossein Morowvat,Ghasem Najafpour Darzi,Aida Iraji,Younes Ghasemi 한국식품과학회 2018 Food Science and Biotechnology Vol.27 No.6
Exopolysaccharide (EPS), as potential microbial base polysaccharide source, has plenty of applications due to its unique physicochemical structure. A Pantoea sp. BCCS 001 GH bacterium with the ability to produce a high amount of EPS was identified by 16S rRNA gene sequencing and biochemical tests. The synthesis of EPS by Pantoea sp. BCCS 001 GH was 13.50 g/L in 48 h when sucrose was used as substrate. The proposed protocol was desirably rapid for massive prodcution of EPS and showed the remarkable impact of sucrose and disodium hydrogen phosphate, peptone, Triton x-100 and 2% (v/v) inoculum size on the yields of EPS production. The EPS was mainly composed of glucose and galactose in a relative molar ration (glucose/galactose) of 85.18:14.82, respectively. The preliminary characterization showed the average molecular- weight of EPS is about 2.522 9 106 Da. The microscopics morphology of polymer was formed irregularly shaped structures.
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