인체 백혈병세포에서 매실 추출물에 의한 apoptosis 유도

You Jeong Chung(정유정),Cheol Park(박철),Yong Kee Jeong(정영기),Yung Hyun Choi(최영현) 한국생명과학회 2011 생명과학회지 Vol.21 No.8

예로부터 한국, 일본 및 중국에서 민간처방 약재 및 건강식품으로 사용되어온 매실은 다양한 효능을 가지는 것으로 알려져 있으나 인체 암세포에서 유발하는 항암작용 및 그에 따른 분자생물학적 기작에 대해서는 명확히 밝혀져 있지 않다. 본 연구에서는 인체 혈구암 U937 세포에서 매실의 메탄올 추출물(MEPM)이 유발하는 항암효과 및 항암기전을 조사한 결과, MEPM 처리 농도 의존적으로 암세포의 성장억제 및 apoptosis를 유발하는 것으로 나타났다. MEPM에 의해서 유발되는 apoptosis에는 XIAP 및 survivin 등과 같은 IAP family의 발현 감소와 더불어 FasL의 발현 증가, Bcl-2의 발현 감소 및 Bid의 단편화 현상이 관여하는 것으로 나타났으며, 두 가지 apoptosis 유발 개시 및 최종 apoptosis 단계에서 중요한 역할을 하는 caspase-8과 -9 및 -3의 활성화와 그에 따른 다양한 기질단백질의 발현 감소 및 단편화가 동반되었음을 알 수 있었다. 또한 인위적인 caspase-3의 활성 차단으로 MEPM에 의하여 유발되는 apoptosis가 현저하게 억제되는 것으로 나타났다. 이상의 결과에서 MEPM은 암세포의 chemotherapeutic agent로서의 가능성을 확인하였지만 향후 지속적인 연구를 통하여 활성물질의 동정 및 관련 기전의 비교 등이 지속적으로 이루어져야 할 것으로 생각된다. In the present study, the pro-apoptotic effects of methanol extract of Prunus mume fruits (MEPM) in human leukemia U937 cells were investigated. It was found that exposure to MEPM resulted in growth inhibition in a concentration-dependent manner by inducing apoptosis. The induction of apoptotic cell death in U937 cells by MEPM was correlated with a down-regulation of inhibitor of apoptosis protein (IAP) family, such as X-linked inhibitor of apoptosis protein (XIAP) and survivin, anti-apoptotic Bcl-2, up-regulation of FasL and cleavage of Bid. MEPM treatment also induced the proteolytic activation of caspase-3, caspase-8 and caspase-9, and degradation of caspase-3 substrate proteins, such as poly (ADP-ribose) polymerase (PARP) and β-catenin. In addition, apoptotic cell death induced by MEPM was significantly inhibited by z-DEVD-fmk, a caspase-3 specific inhibitor, which demonstrates the important role of caspase-3 in the apoptotic process by MEPM in U937 cells. Taken together, these findings suggest that P. mume extracts may be a potential chemotherapeutic agent for the control of human leukemia cells and further studies will be needed to identify the active compounds.

신이식 환자에서 골밀도에 대한 연구

최기철(Ki Chul Choi),박종욱(Jong Wook Park),유기섭(Ki Sub Yoo),이민수(Min Su Lee),염충호(Chung Ho Yeum),정유정(You Jeong Chung),이제중(Je Jung Lee),김수완(Soo Wan Kim),김남호(Nam Ho Kim),강영준(Young Joon Kang) 대한내과학회 1996 대한내과학회지 Vol.50 No.2

N/A parameters and BMD of renal transplants. Methods: Using dual-energy X-ray absorptiometry (DEXA), we assessed total body and regional (head, arm, trunk, rib, leg, spine, and pelvis) bone mineral density. Results: 1) Total body BMD of renal transplants was significantly decreased compared with that of normal controls. 2) A separate analysis of BMD in both sexes revealed that males presented marked reduction of BMD compared with the normal controls in the majority of skeletal sites except head and pelvis. Especially, at the spine, their BMD was significantly reduced compared with that of the CRF patients on receiving dialysis in addition to the normal controls. Females also presented reduction of BMD compared with the normal controls in many skeletal sites except head and ribs. 3) The correlations between BMD values and time since renal transplantation and cumulative cyclosporine doses were statistically significant (r=0.46, 0.527, respectively, and p<0.01) in nearly all skeletal sites except head and spine. Conclusion: Our results indicate that significant falls in BMD appear to be inherent to the process of renal transplantation although it achieves the rapid correction of some of the biochemical abnormalities associated with renal failure and that therapeutic strategies to prevent or reduce this damages should be established. In addition, the present study emphasizes the need to pay attention to the further evaluation of correlations between bone loss in renal transplants and some drugs, such as glucocorticoids and cyclosporine.

지속적외래복막투석 환자 250 예의 임상적 고찰

최기철(Ki Chul Choi),유기섭(Ki Sud Yoo),박종욱(Jong Wook Park),하정훈(Jeong Hoon Ha),이제중(Je Jung Lee),염충호(Chung Ho Yeum),정유정(You Jeong Chung),김수완(Soo Wan Kim),김남호(Nam Ho Kim),강영준(Young Joon Kang) 대한내과학회 1996 대한내과학회지 Vol.51 No.3

N/A Objectives: CAPD has established itself as an effective therapeutic modality as hemodialysis or renal transplantation in the treatment of patients with end stage renal disease. Although much progress of technology of CAPD has been made, peritonitis or catheter-related complications have been an important problem until now. Therefore, we reviewed our experience with CAPD over 6.5 years. Methods: Retrospectively, we analysed the clinical results of 250 patients on CAPD at Chonnam University Hospital from July 1988 to December 1994. Results: 1) There were l48(59%) male and 102(41%) female patients, aged 10-73 years(mean 43±13 years). 2) Underlying disorders of end stage renal diseases consisted of mainly chronic glomerulonephritis (24.4%), followed by diabetic nephropathy(22.4%) and hypertensive nephrosclerosis(7.2%). 3) The incidence of pertionitis was 0.75 episodes per patient year and peritonitis free interval was 9.0±1.2 months. The isolation rate of microorganisms from patients with peritonitis was 34.7% and the order of frequency of isolated organisms was Staphylococcus(S.) epidermidis, S. aureus, Pseudomonas species, Candida species, Acinetobacter species and E. coli. The results of peritonitis were treatment with only antibiotics(86.0%), catheter removal(13.0%) and death(1.0%). 4) The complications other than peritonitis were exit site infection or tunnel infection, hernia, leakage, failure to drain, hydrothorax, ultrafiltration failure and others. 5) 67 of 250 catheters(26.8%) were removed and the peritonitis was the most common cause of catheter removal. The catheter survival rate was 86%, 75%, 65%, and 52% at one year, two years, four years and five years, respectively. Mean survival time of all catheters was 44.2±1.6 months. 6) The causes of death were most frequently cardiac disorders(31.8%), followed by vascular disorders (19.5%), and infections(14.6%). The patient survival rate was 94%, 83%, 74%, 69% at one year, two years, three years, four years, respectively. Mean survival time of all patients was 59.0±2.0 months. Conclusion: Although CAPD is an effective therapeutic modality in the tgeatment of patients with end stage renal disease, CAPD-induced peritonitis is the most important pitfalls of CAPD. Therefore, we must exert more effort to prevent and treat the CAPD-induced peritonitis.

온돌난방에서 노인과 청년의 피부온 반응 비교

최정화,정유정 한국의류학회 1998 한국의류학회지 Vol.22 No.1

The purpose of this study was to investigate the effect of age on the human thermoregulatory responses and to obtain the basic information of the Korean thermophysiological characteristics under the traditional Korean floor heating system-Ondol. The participants consisted of 10 elderly women, 4 elderly men(over 65 years), 10 young women and 5 young men(in twenties). They were exposed to temperature-controlled Ondol room(20±1℃, 50±5%R.H., floor surface temperature: 30±1℃) for 60 minutes and wore the same experimental clothing. The postures were performed sitting and supine on the floor. In each pose, rectal temperature, skin temperature of 10 areas, mean skin temperature, clothing microclimate, body fat were measured. The results were as follows; 1. The rectal temperature gradually decreased and mean skin temperature gradually increased in young and old groups in both supine and sitting pose on heated Ondol floor for 60 minutes. 2. Rectal temperature was different in each pose. The rectal temperature decreased 0.1℃ in both groups under the condition of sitting and decreased 0.2℃ in elderly group under the condition of supine. In supine pose, elderly group was more declined than young group. Mean skin temperature increased 0.5℃ in both poses, both groups.