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김광세,Yong-KilHong,YoonLee,Joo-YoungShin,Soo-IkChang,SooIlChung,YoungAeJoe 생화학분자생물학회 2003 Experimental and molecular medicine Vol.35 No.6
The serine protease urokinase-type plasminogen activator (uPA) is im plicated in pericellular proteolysis in a variety of physiological and pathological processes including angiogenesis and tumor metastasis. The kringle domain of uPA (UK1) has proven to be an anti-angiogenic molecule with unknown mechanism and amino terminal fragment of uPA (u-ATF) with additional growth factor-like domain can be used for blocking interaction of uPA and uPA receptor. Here, we compared anti-angiogenic activities of these two molecules in vitro and in vivo. The recombinant u-ATF from E. coli and refolded in vitro was found to bind to uPAR with high affinity, whereas E. coli-derived UK1 showed no binding by Biacore analysis. In contrast to UK1 having potent inhibitory effect, u-ATF exhibited low inhibitory effect on bovine capillary endothelial cell growth (ED50>320 nM). Furthermore, u-ATF inhibition of VEGF-induced migration of human umbilical vein endothelial cell was far less sensitive (IC50 = 600 nM) than those observed with UK1, and angiogenesis inhibition was marginal in chorioallantoic membrane. These results suggest that kringle domain alone is sufficient for potent antiangiogenic activity and additional growth factor-like domain diverts this molecule in undergoing different mechanism such as inhibition of uPA/uPAR interaction rather than undergoing distinct antiangiogenic mechanism driven by kringle domain.
Yoon Lee,So-Young Lim,Hong-Joo Jung 한국무역금융보험학회(구 한국무역보험학회) 2020 무역금융보험연구 Vol.21 No.6
본 논문은 핀테크 기반 해외송금업체들의 주요한 특징을 확인하고 해외송금을 주로 이용하는 외국인 근로자들이 선호하는 송금방식과 이에 영향을 주는 요인들을 분석하였다. 이를 위해서 해외송금 방식들의 특징, 송금의 간편성, 경제성을 확인하고, 외국인 근로자들에게 진행한 설 문조사 결과 중, 선호하는 해외송금방식과 이에 영향을 주는 요인, 만족도에 대해서 카이제곱 검정에 의한 교차분석을 시행하였다. 분석 결과, 자주 사용하는 송금방식에 연령, 송금액, 송금 빈도, 근무지가 연관성을 보였다. 연령이 높고, 송금액이 크며, 송금빈도가 낮을수록 은행의 해외송금을 선호하였으며, 연령이 낮고, 송금액이 작으며, 송금빈도가 높을수록 송금전문업체 의 해외송금 서비스를 사용하는 것으로 나타났다. 그리고 서비스에 대한 만족도는 송금전문업 체가 은행보다 더 높게 나타나서 은행의 서비스 개선의 필요성도 확인할 수 있었다. Purpose : The objective of this study is to examine the major characteristics of oversea remittance methods and analyze the influencing factors of the overseas remittance methods. Research design, data, methodology : The analysis was based on a survey on foreign workers in Korea who are the main users of the remittance service. Chi-squared test was used to find the influencing factors for the remittance method choice. Results : The result showed that the age, remittance amount, frequency of remittance, and workplace were related to the frequently used remittance method. Conclusions : The lower the age, the smaller the amount of remittance, and the higher the frequency of remittance, the more remittance service of the money transfer company was preferred. Also, as the satisfaction level of the bank is lower than MTO, the bank needs to improve its services to survive in the competition.
Effect of moisture and drying time on the bond strength of the one-step self-etching adhesive system
Yoon Lee,Jeong-Won Park 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.3
Objectives: To investigate the effect of dentin moisture degree and air-drying time on dentin-bond strength of two different one-step self-etching adhesive systems. Materials and Methods: Twenty-four human third molars were used for microtensile bond strength testing of G-Bond and Clearfil S3 Bond. The dentin surface was either blot-dried or air-dried before applying these adhesive agents. After application of the adhesive agent, three different air drying times were evaluated: 1, 5, and 10 sec. Composite resin was build up to 4 mm thickness and light cured for 40 sec with 2 separate layers. Then the tooth was sectioned and trimmed to measure the microtensile bond strength using a universal testing machine. The measured bond strengths were analyzed with three-way ANOVA and regression analysis was done (p = 0.05). Results: All three factors, materials, dentin wetness and air drying time, showed significant effect on the microtensile bond strength. Clearfil S3 Bond, dry dentin surface and 10 sec air drying time showed higher bond strength. Conclusions: Within the limitation of this experiment, air drying time after the application of the one-step self-etching adhesive agent was the most significant factor affecting the bond strength, followed by the material difference and dentin moisture before applying the adhesive agent. (Restor Dent Endod 2012;37(3):155-159)
Yoon Lee,Sujin Choi,Ben Kang 대한소아소화기영양학회 2023 Pediatric gastroenterology, hepatology & nutrition Vol.26 No.1
Purpose: Total colonoscopy is recommended if colorectal polyps are clinically suspected. This study aimed to investigate the performance status of pediatric colonoscopic polypectomy in Korea. Methods: We surveyed pediatric endoscopic specialists who perform colonoscopic polypectomy in Korea using a questionnaire of 13 questions on pediatric colonoscopic polypectomy performance status. Results: The survey was conducted at 45 institutions, and 32 specialists (71.1%) responded. Among the respondents, 31.2% (10/32) said colonoscopy was performed in all age groups, while 12.5% (4/32) said sigmoidoscopy was performed in all age groups. Meanwhile, 56.2% (18/32) said that sigmoidoscopy was performed in young children, while colonoscopy was performed in older children. Among them, 38.9% (7/18) believe that 4-6 years were young, and 44.5% (8/18) believe that 7-9 years were young. Regarding surveillance examinations, 21.9% (7/32) said they would perform a surveillance colonoscopy or sigmoidoscopy in the future if less than five juvenile polyps were found in the colon. Meanwhile, if less than five adenomatous polyps were found in the colon, 93.8% (30/32) said they would perform surveillance colonoscopy or sigmoidoscopy in the future. Conclusion: More than half of the pediatric endoscopic specialists in Korea choose between a colonoscopy and sigmoidoscopy depending on the patient’s age, contrary to the generally accepted recommendation of total colonoscopy if colorectal polyps are suspected in children and adolescents. In this survey, most pediatric endoscopists used the age range of 4-9 years as the reference age.
The Effectiveness of Language Learning Based on the Knowledge Structure Framework
Yoon Lee 한국초등영어교육학회 2003 초등영어교육 Vol.9 No.2
When children learn language, it is important to know what they are learning as well as how it is dealt with in the learning process. Knowledge structure is about how people organize their knowledge of the world so as to understand and retain new information. Since all contents have knowledge structures, a content can be learned by its knowledge structure framework. This study is to explore the effectiveness of language learning based on the knowledge structure framework, and to investigate how a graphic organizer can facilitate the learning of knowledge structure. In order to do this, a nine-year old Korean immigrant boy was taught with texts based on the knowledge structure framework, and aided by graphic organizers in the learning process. The learning processes were recorded onto audio tapes and were later transcribed to examine the linguistic devices the student used. This study revealed that the student showed improvement in language and content learning, which had been taught using the knowledge structure framework, and the graphic organizer played a key role in 'scaffolding'. It is suggested that English should be taught in a meaningful context, and this emphasis on content should be applied to curricula as well as textbooks.