韓國産 집먼지진드기類(먼지진드기科)의 分類學的 硏究

盧鏞泰,孫秉玉,申炳湜 건국대학교 교육대학원 1988 敎育論叢 Vol.10 No.-

The house dust mites were isolated from houses, dust of civil house, public facilities and lodging ahouses in Seoul, Chinan, Yungnam and several other areas during the period of June to October, 1985. The isolated house dust mites were identified into 3 species(Dermatophagoides pteronyssinus, D.farinae, Euroglyphus maynei) belonging to 2 genera, 2 subfamilies. Their larvae and nymphs were decribed. Variation of the epigynium of females and other characteristics were examined and redescibed. And the key to korean house dust mites was made.

진드기 Haemaphysalis japonensis, Warburton과 Ixodes ovatus, Neumann의 Haller씨 기관에 관한 주사전자현미경적 연구

盧鏞泰 최신의학사 1983 最新醫學 Vol.26 No.6

甲殼類 十脚目의 腸上皮細胞 微細構造에 관한 硏究

盧鏞泰,延根聖 建國大學校基礎科學硏究所 1988 理學論集 Vol.13 No.-

甲殼類의 十脚目 중, 민물에 서식하는 가재(Cambaroides similis Koelbel)와 민물과 바다를 생태권으로 하는 참게(Eriocheir sinensis H. Milne Edwards) 그리고 바닷가 갯벌에 서식하는 칠게( Macrophthalmus (Mareotis) japonicus De Haan)를 재료로 주사 전자현미경과 투과 전자현미경에 의한 장상피 세포의 미세구조를 관찰하고 다음과 같은 결과를 얻었다. 1. 장상피 세포는 전반적으로 핵이 기부 가까이 위치한 단층 원주상피 세포로 되어 있고 중장의 길이는 가재가 0.8∼1.0mm(중 ·후장 길이의 1/25)로 극히 짧고, 참게는 20∼30mm(중 ·후장 길이의 2/5)로 가장 길며 칠게는 5∼8mm(중 ·후장 길이의 1/5)이다. 2. 중장 내벽은 3종 모두 체축에 따라 잔잔한 주름이 많이 잡혀져 있고, 후장의 두꺼운 키틴질 표면층에서는 2∼20㎛의 돌기가 2∼50개 정도로 선상 또는 집단을 이루고 뒤쪽을 향해 나 있어 장 속의 물질을 뒤로 이동시키는 작용을 한다. 3. 중장은 원주상피 세포로 되었고 대형의 엽상핵이 기저부 가까이 위치한다. 정단 상피세포의 자유표면에 가까운 세포질에는 미토콘드리아가 밀집하고, 핵 주변에는 많은 조면소포체가 분포하며 세포질 전반에 유리 리보소옴이 짙게 분산되어 있다. 가재와 칠게는 자유표면에 솜털피막이 발달하고 측면 세포막에는 interdigitating cell processes가 발달하고 있으며, 참게는 자유표면에 솜털피막이 발달한 미세융모가 밀생하고 측면 세포막은 occuluding junction과 intermediate junction이 있어 3종 모두 흡수 및 분비기능을 가지고 있다. 4. 후장은 종에 따라 차이가 크게 나타났다. 가재는 기저부 가까이 卵形의 핵을 가진 원주세포 자유표면에 솜털피막이 발달한 긴 미세융모, 그 안쪽 세포질에 밀집된 미토콘드리아, 핵 주변에 나열된 많은 조면소포체 및 측면 세포막의 interdigitating cell processes의 발달로 흡수기능을 가지고 있다. 참게는 작은 미세 융모가 발달한 상피세포의 자유표면 안쪽 세포질에 밀집된 미토콘드리아, 세포질 전반에 분산된 많은 유리 리보소옴, 측면 세포막의 interdigitating cell processes, 심한 기저막의 함입과 많은 분비과립의 발달이 흡수, 분비 및 활발한 수분출입을 나타낸다. 칠게는 卵形 핵이 기저부 가까이 위치하며 크고 긴 미토콘드리아가 핵 주변에서 자유표면까지 분포되고 전자밀도가 높은 暗세포와, 미토콘드리아가 자유표면 밑에 주로 분포되고 전자밀도가 낮은 明세포가 구분된다. 후반부 상피세포는 분비입자, 분비과립, 핵 기부에 분포된 미토콘드리아, 기저막의 함입 및 세포간극의 발달로 무기물과 수분의 활발한 대사기능을 가지고 있다. 핵 주변의 미토콘드리아는 크리스테가 세로로 배열되어 있다. The studies of the scanning electron microscopy and the ultrastructure of the intestinal epithelia on three species of decapod crustacea, fresh water crayfish(Cambaroides similis Koelbel), fresh water crab(Eriocheir sinensis H.Milne-Edwards) and shore crab (Macrophthalmus (Mareotis) japonicus De Haan)were performed in this thesis. The results obtained were as follows: The mid-and hindgut lumens of the three species are lined by a simple columnar epithelium with the basally located nucleus. The lengths of the midgut are approximately 0.8mm-1.0mm long in the fresh water crayfish, 20mm-30mm long in the fresh water crab and 5mm-8mm long in the shore crab. Scanning electron microscopy: The midgut epithelial lining is covered with mucosubstances and folded longitudinally. The hindgut epithelium is lined with chitinous layer, from whose surface the chitinous spines numbered from a few to fifty (two-twenty um long) are extruded in a group or linear arrangement and directed posteriorly. The spines make the fecal substances move afterward easily. Ultrastructure: The midgut epithelial cells of the three species are characterized by a fuzzy coated striated border, dense mitochondria in the apical cytoplasm, a large amount of rough endoplasmic reticulum around the lobed shape of nucleus, free ribosomes, interdigitating cell processes of lateral cell membrane and the junctional structure. These diverse features indicate the function of an absorption and a secretion. The hindgut epithelial cells differ from each species observed. The epithelial cells of the fresh water crayfish are characterized by the oval shaped nucleus located basally, fuzzy coated long microvilli, rough endopalsmic reticulum around the nucleus and interdigitating cell processes of lateral cell surface, which indicate an activity in an absorption. The epithelial cells of the fresh water crab are characterized by condensed mitochondria under the apical striated border, free ribosomes scattered in cytoplasm, interdigitating cell processes of lateral cell surface, a great developed basal infoldings and the numerous secreting vesicles which indicate an activity in an absorption, a secretion of ions and water transport. The epithelial cells of the shore crab are distinguished into two cell types. The electrondense dark cells contain long and large mitochondria around the nucleus to the apical cell surface, more rough endoplasmic reticulum and free ribosomes than the electron-lucent light cells. The epithelial cells in the distal portion of the hindgut contain secretory particles, secretory vesicles, numerous mitochondria around the nucleus, basal infoldings and intercellular apaces, which indicate an sctivity in water and ions transport. The mitochondria around the nucleus have moderate dense longitudinal cristae.

Tilapia의 空腹狀態와 飽食量과의 關係

金鍾連,盧鏞泰 군산대학교 1993 群山水産專門大學 硏究報告 Vol.27 No.1

The effect of the time of food deprivation on the satiation amount, the digestion of food in the stomach of Tilapia fingerling(mean total length 8.6cm, body weight 10.63g) and the relationship between the digestion of food in the stomach and the satiation amount, are examined. The satiation amount of a school of Tilapia fingering depends on the degree of hunger. As the time of food deprivation increases, the satiation amount first increases and then levels off to a constant value(mean satiation amount per fish, 0.816 g). Futher, when the time of food deprivation is very long, about 8 days, the satiation amount does not exeed the constant value but the amount rather decreases. At the beginning of satiation the amount of food in the stomach sharply decreases. This rate of decrease levels off in later stages and a considerable time(22∼24 hours) elapses before the stomach is completely empty. If undigested food remains in the stomach the state cannot be referred to as being complete hunger. Until the food in the stomach is completely digested, the satiation amount varies inversely with the amount of undigested food in the stomach. The satiation amount reaches its peak value as the stomach contents approach zero. Since the capacity of the stomach is limited, the satiation amount after reaching its maximum at the state of complete hunger maintains this maximum value even when the time of food deprivation is further extended for a short period of time.

무당개구리 정자형성의 세포학적 연구

金翰華,盧鏞泰,池永得,文英花 최신의학사 1982 最新醫學 Vol.25 No.5

칠게(Macrophthalmus (Mareotis) japonicus De Haan)의 腸上皮의 徵細構造 및 組織化學的 硏究

延近聖,盧鏞泰 建國大學校基礎科學硏究所 1987 理學論集 Vol.12 No.-

칠게(Macrophthalmus(Mareotis) japonicus De Haan)의 腸上皮에 대한 走査電子顯微鏡, 透過電子顯微鏡的 觀察과 粘液質에 대한 性狀을 組織化學的으로 밝히고자 Haematoxylin-Eosin 염색, Periodic acid-Schiff 반응, Alcian blue pH 2.5와 0.4염색, Aldhyde fuchsin pH 1.7염색 및 Aldehyde fuchsin pH 1.7-Alcian blue pH 2.5염색을 시행하고 분석한 결과 다음과 같은 결론을 얻었다. 1. 中腸과 後腸의 길이의 대비는 약 1:4정도였다. 2. 中腸에는 얇은 粘液層이 덮인 가운데 上皮細胞의 頂端面은 細胞膜의 많은 灣入을 이룬 내측에는 미토콘드리아가 집중적으로 밀집하고 있었으며 가끔 全分泌細胞의 분비현상이 나타나고 있다. 3. 後腸에는 두꺼운 키틴질층이 腸腔에 면하고 있는 上皮表面을 덮고 있으며 키틴질의 表層에서는 2μm∼15μm에 이르는 돌기들이 部位에 따라 수와 분포양상을 달리하며 모두 뒷쪽을 향해 나있어 後腸壁에 발달한 근육층의 운동에 의한 물질의 後送을 도와주는 것으로 보였다. 4. 後腸上皮의 頂端細胞膜에서는 다소 작으나마 細胞膜의 많은 灣入과 그 내측에 밀집된 미토콘드리아의 분포가 막의 활발한 투과력을 보여주었으며 많은 분비과립의 분비를 볼 수 있었다. 5. 幽門胃壁을 덮고 있는 粘液質은 表層에 中性粘液質과 약간의 sialomucin을 포함한 sulfomucin으로 되어 있었고 內層은 약간의 中性粘液質에 sialomucin으로 되어 있었으며 內層 粘液質은 中性粘液質과 sialomusin만으로 되어있다. 6. 中腸은 中性粘液質에 약간의 sulfomucin이 섞인 상태이며 sialomucin은 전체적으로 나타나지 않았다. 7. 後腸은 中性粘液質이 sulfomucin보다 우세하게 섞여있는 상태였고 sialomucin은 역시 나타나지 않았다. The study on the ultrastructure, scanning electron microscopy and histochemical analysis was performed to the shore crab (Macrophthalmus (Mareotis) japonicus De Haan)(Crustacea, Decapoda). For the chemical study, haematoxylin-eosin staining, periodic acid-Schiff reaction, alcian blue pH 2.5 and pH 0.4 staining, aldehyde fuchsin pH 1.7 staining and aldehyde fuchsin pH 1.7-alcian blue pH 2.5 staining were carried out. The general types of mucosubstances detected were identified as neutral mucosubstance, sulfomucin and sialomucin. The results were as follows. 1. The length of midgut was approximately one-fifth of the total length of the mid and hind gut. 2. The midgut epithelium was covered with thin layer of mucosubstance. The epithelium in the apical border formed striated border under which the mitochondria(M) concentrated with high density. Holocrine cells could often be seen. 3. Hindgut epithelium lined by a chitinous layer which had a number of spines grouped linear arrangement numbered from a few to about thirty extruding from the upper part of the lining which directed posteriorly. The spines seemed to make the fecal substances move afterward easily by the densely distributed muscular movement of the gut. 4. The apical border of the hindgut epithelial cells appeared striated short under which the concentrated mitochondria were seen. Secretory granules scattered outside the cell membrane. 5. The upper chitinous layer of the epithelium of the pyloric stomach contained neutral mucosubstance and sulfomucin with a little amount of sialomucin. The lower chitinous layer of the epithelium consisted of a neutral mucosubstance and sialomucin. Sulfomucin was absent. 6. The midgut epithelium covered with a thin mucous layer consisted of a neutral mucosubstance with a some sulfomucin. Sialomucin couldn't be identified. 7. The chitinous layer of the hindgut epithelium showed a strong PAS positivity and fuchsinophility, and no alcianophility. It appeared to had a mixture of neutral mucosubstance and sulfomucin.

A New Entonyssid Mite (Acari: Entonyssidae) from Korea

Noh, Yong-Tai,Sohn, Byung-Ok 한국곤충학회 1991 Entomological Research Vol.21 No.1

건국대학교 생물학과 동물표본실에 보관된 진드기류의 표턱을 동정한 결과 Entonyssidae의 1신종이 밝혀져 Entonyssus koreansis로 명명하고 기재보고한다. Entonyssusu koreansis, n. sp. (Acari: Entonyssidae) is described from the lung of the snake, Natrix trigrinal lateralis (Berthold) from near Choonchun in Korea.

A Taxonomical Study in Ticks in Korea(Ⅵ) : Ixodes nipponensis Kitaoka and Saito (IXODIDEA, IXODIDAE)

Noh, Yong Tai,Cho, Baik Kee 최신의학사 1983 最新醫學 Vol.26 No.9

韓國産 鳥類 진드기 (I)

Noh, Yong-Tai The Korean Society for Integrative Biology 1969 동물학회지 Vol.12 No.2

韓國産 鳥類에 寄生하는 진드기에 關하여서는 지금까지 硏究된바 없다. 著者는 韓國産 鳥類 진드기를 採集 同定한 結果 Ixodes signatus 가 韓國未記錄種임이 밝혀졌으므로 이에 發表한다.

한국산 오디흑연가시, Chordodes koreansis, (철선충목, 유선형동물문)의 형태학적 연구

노용태 ( Yong Tai Noh ),백광민 ( Kwang Min Baek ),문인호 ( In Ho Moon ) 건국대학교 기초과학연구소 1991 理學論集 Vol.16 No.-

The morphological character of Chordodes koreansis was studied with light microscope, transmission and scanning electron microscope. The body is slender and elongated, the males are shorter than the females. The body wall consist of cuticular layer, subcuticular layer and muscle layer. The epicuticle is a thin superficial layer whose surface shows the rows of polygonal elevation. Alternating layers of fibers cross one another diagonally and most of cuticle is filled with fibers layer. The subcuticular layer is a single layer varing from a low cuboidal to a columnar shape. The muscle layer consists of longitudinally arranged each cell containg many filaments. The digestive tract is more or less degenerated. A patent mouth is opened to the foregut and the midgut is a lump of simple epitherial cells lying in the pseudocoel. The midgut is surrounded by unidentified tissue. The sexes arc separated and the gonads consist of a pair of cylindrical bodies running the body length in both sexes. There is no genitalia in both sexes. In males, each testis continues posterioly and enters the cloaca separately from its follow by a way of short duct deferens. In females, the reproductive system consists of a pair of ovary, oviduct, single uterus and seminal receptacle. The pseudocoel is filled with parenchymal cells and gonad. The parenchymal cells are the irregular, polygonal or spindle-shape cells. Enclosing the parenchymal cells is a basal lamina, and external to that is the collagenous ma trix that isolates the parenchymal cells from one another.