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Yong-Seung JOUN,KwangSoon CHOI,YeRin KIM,Kee-Young LEE,Eunjin SHIN,Tae-Heon KIM,Hyun KANG,Kimoo LEE,SangMoon KANG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
Since peptides are human-friendly and safe substances, they are widely used in pharmaceutical and cosmetic field, in addition, many peptides show high physiological activity and their use is increasing due to the fact that they are easy to synthesize or modify. Peptidomimetics are compounds whose essential elements mimic a natural peptide or protein in 3D space and which retain the ability to interact with the biological target and produce the same biological effect. Many peptides undergo a peptidomimetic process to improve efficacy or enhance stability. such as acetylation, palmitoylation, and are representative variations. In this study, we performed a peptidomimetic process to improve the thermal stability and enzyme resistance of peptides, to improve thermal stability, we studied peptide modifications such as acetylation and palmitoylation and to improve the enzyme resistance to attempts to deformation, such as D-amino acid exchange, cyclization. Each of the modified peptides was confirmed to have improved thermal stability and enzyme resistance, and it was confirmed that there was no cell cytotoxicity and the efficacy was equal to or higher than that of the original peptide.
Cellular-uptake Behavior of Polymer Nanoparticles into Consideration of Biosafety
Do, Jeong-Hoe,An, Jeong-Ho,Joun, Yong-Seung,Chung, Dong-June,Kim, Ji-Heung The Polymer Society of Korea 2008 Macromolecular Research Vol.16 No.8
Nanoparticles have tremendous potential in cancer prevention, detection and augmenting existing treatments. They can target tumors, carry imaging capability to document the presence of tumors, sense pathophysiological defects in tumor cells, deliver therapeutic genes or drugs based on the tumor characteristics, respond to external triggers to release an appropriate agent, document the tumor response, and identify the residual tumor cells. Nanoparticles < 30 nanometers in diameter show unexpected and unique properties. Furthermore, particles < 5 nanometers in size can easily penetrate cells as well as living tissues and organs. This study evaluated the safety of nano materials in a living body and the relationship between the living tissue and synthetic nano materials by examining the in-vitro cytotoxicity of poly(lactic-co-glycolic) acid (PLGA) nano-spheres and fluorescein isothiocynate(FITC)-labeled dendrimers as polymer nanoparticles. PLGA was chosen because it has been used extensively for biodegradable nanoparticles on account of its outstanding bio-compatibility and its acceptance as an FDA approved material. The dendrimer was chosen because it can carry a molecule that recognizes cancer cells, a therapeutic agent that can kill those cells, and a molecule that recognizes the signals of cell death. Cytotoxicity in L929 mouse fibroblasts was monitored using MTT assay. Microscopic observations were also carried out to observe cell growth. All assays yielded meaningful results and the PLGA nanoparticles showed less cytotoxicity than the dendrimer. These nano-particles ranged in size from 10 to 100 nm according to microscopy and spectroscopic methods.