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이담자효모 Rhodosporidium toruloides의 성pheromone(Rh.A)에 의한 성접합형 특이적 인산화 저해 반응
정영기 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
Two phosphorylated proteins having molecular weights of 57kD and 72kD were detected from the solubilized membrane protein fraction of mating type a cells of Rhodosporidium toruloides which belongs to heterobasidiomycetous yeast. The phosphorylation of the protein was inhibited by a sexual pheromone, Rhodotorucine A (Rh. A), which is secreted from mating type a cells. On the other hand, counterpart mating type A cells and M-39 strain which is a sterile mutant derived from a cells, had also the same two phosphorylated proteins. However, the phosphorylation of the protein from A cells and M-39 strain were not inhibited by the Rh. A. It suggests that inhibition of the phosphorylation reaction by the Rh. A in mating type a cells is a mating-type-specific reaction that relate to transduction mechanism of sexual pheromone signaling.
Bacillus sp. YJ-63이 생산하는 항곰팡이 항생물질의 구조분석
정영기,신영준,정명주,주우홍,최재수 Institute of Genetic Engineering Changwon National 2002 Gene and Protein Vol.6 No.-
토양으로부터 분리한 Bacillus sp. YJ-63으로부터 항진균성 항생물질을 분리하여 그 구조를 분석한 결과는 다음과 같다. 항생물질은 7개의 α-amino acid와 1개의 β-amino acid가 결합한 환상구조를 가지는 iturin E 계에 속하며 특히, β-amino acid의 methylene 수가 기존의 iturin E 보다 더 많은 것으로 나타났다. Structural analysis was performed by the ^1H-NMR, ^13C-NMR, amino acid composition analysis and FAB-mass. The instrumental analysis represented that the potential antifungal antibiotic belonged to the iturin E group antibiotic, consisting of 7 α-amino acid residues and a collection of β-amino acid with aliphatic side chain. Compared to the iturin E group, notably, the potent antifungal antibiotic from bacillus sp. YJ-63 carried longer β-amino acid side chain. In conclusion, these findings identified a potential antibiotic, which contained a stable cyclopeptide structure with long β-amino acid side chain.
해양미생물로부터 카로테노이드 색소의 생산을 위한 최적조건
정영기,박정욱,주우홍,류병호,정명주,김태수 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
The optimal medium composition for the production of carotenoid pigment from Haloarcular sp. EH-1 as a dietary for fishes were 1.0% sucrose, 1.0% yeast extract, 25% sodium chloride, 0.3% sodium citrate, 0.2% potassium chloride, 2.0% magnesium sulphate, 0.002% ferric sulphate(pH7.0). The incubation temperate, aeration rate and agitation speed were 40℃, 100ml medium/500ml vol. shaking flask, and 180 rpm/min. The carotenoid pigment production was highest after 5 days of incubation with the light.
이담자 효모의 세포간 성응집의 특성과 표면단백질의 관련성
정영기,이태호,최용락,강원대 동의대학교 기초과학연구소 1996 基礎科學硏究論文集 Vol.6 No.1
When mating type A and a cells of heterobasidiomycetous yeast Rhodosporidium toruloides were mix-cultured, both of the mating type cells have shown strong agglutination. But this agglutination was not detactable when the A and a cell were cultured separately. From reagglutination made just after the result of disassembling the agglutination by sonication, we knew that the agglutination was sexual-agglutination, not simple physical cell agglutination. The sexual agglutination was progressed actively on logarithmic phase and, in addition, progressed faster on mating type a cell treated with rhodotorucine A. These sexual agglutination have been inhibited by several protease such as trypsin, pronase, chymotrpysin and thermolysin and inhibited by 5 mM DTT as well.
이담자균 효모 Rhodosporidium toruloides에서 Rhodotorucine A에 의한 막단백질 인산화의 저해와 Trigger Peptidase의 관련성
정영기,이태호,류병호 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1
[γ-^32P]ATP was used to test phosphorylation of membrane proteins of mating type a cells of heterobasidiomycetous yeast Rhodosporidium toruloides separated by non-denaturing electrophoresis. The phosphoprotein was observed in the membrane proteins. The phosphorylation was inhibited by the pheromone rhodotorucine A (Rh. A) secreted by mating type A of the yeast. Rh. A didn't inhibit the phosphorylation in the presence of a trigger peptidase (TPase) inhibitor, antipain. Partially digested Rh. A by trypsin maintained the phosphorylation inhibitory activity. These results show that TPase activity plays an important role in the transduction of pheromone signal in the yeast.
이담자효모 Rhodosporidium toruloides의 막단백질 인산화와 성 Pheromone, Rhodotorucine A의 작용
정영기 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1
When the membrane protein fraction of mating type a cells of heterobasidiomycetous yeast R. toruloides was phosphorylated in vitro, two phosphorylated proteins of 72Kd and 57Kd were detected on SDS-polyacryamide gel. The phosphorylation reaction was inhibited by rhodotorucine A(Rh. A) which is a sexual pheromone secreted by mating type A cells. The inhibition of phosphorylation by Rh.A was dependent on Ca^2+, and independent on Mg^2+ or calmodulin. When adding trigger peptidase (TPase) inhibitor, antipain, no inhibition of phosphorylation was observed. Also, by adding the trypsin-digested product of Rh. A, the phosphorylation was inhibited as the action of Rh. A. From these results, it is expected that the inhibition of membrane protein phosphorylation should be caused by the digested product of Rh. A with TPase.
정영기,이재준 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
The purpose of the paper is to explore the current knowledge on the nutritional evaluation, cholesterol-lowering effect and antitumor activity of kimchi and its ingredients(Korea cabbage, garlic, red pepper powder, ginger and onion). Kimchi contains high contents of nutrients such as vitamins(ascorbic acid, β-carotene and vitamin B complex), minerals(calcium, potassium, iron and phosphorous), essential amino acids and dietary fiber. Kimch also contains high levels of lactic acid bacteria, allicin, capsaicin, organic acid, phenol compounds, flavonoid and sulfur compounds. The dietary fiber and lactic acid bacteria isolated from kimchi are effective in improving intestinal microflora of human. Insoluble dietary fiber shows anticancer activity, but soluble dietary fiber shows hypocholesterolemic effect. Lactic acid bacteria isolated from kimchi acts as a hypocholesterolemic or anticancer agent. A major ingredient of kimchi is mainly cruciferous and allium family vegetables, which were also reported to prevent cancer and atherosclerosis. It is suggested that kimchi is important not only as one of traditional fermented Korea foods but also as therapeutic agent for carcinogenesis and hypercholesterolemic state.
Van der Waals集團에 대한 電子ion化와 電子부착
鄭圻柱,金容福,李熙福 공주대학교 사범대학 과학교육연구소 1998 과학교육연구 Vol.29 No.1
초음파 확장에 의한 van der Waals집단에 대한 電子想互關係는 二重焦點 質量分析器로 硏究되어 왔다. 특별히 本 硏究에서는 ??집단 이온의 생성과 안정성 그리고 O₂와 CO₂집단에 대한 電子부착을 零에너지에 접근하는 고효율 電子부착기로 실시하였다.
이담자균 효모의 성분화 과정중 인지질의 작용과 배지조성의 제한이 성분화에 미치는 영향
정영기,강원대,남수완 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1
The action of phospholipid on the rhodotorucine A(Rh.A) acceptance by heterobasidiomyceteous yeast Rhodospori-dium toruloides mating type a cells and the effect of medium composition during sexual differentiation were investigated. Activation of trigger peptidase(TPase) was very sensitive to the originated phospholipid from R. toruloides and was more sensitive to phospholipid liposome made up of phospholipid. Phospholipid present on the membrane of mating type a cells consists of phospatidylglycerol(PG), phosphatidylethanolamine(PE), phospatidylcholine(PC), phospatidylinositol(PI), and phosphatidylserine(PS) of 12.9, suprisingly 45.4, 11.0, and 13.9%, respectively. As the result of using C-1 and N-1 mediums which limited C and N sources capable of inhibiting the synthesis of phospholipid. it resulted inhibiting sexual differentiation and production of Rh.A from mating type A cells.
정영기,최병대 동의대학교 기초과학연구소 2000 基礎科學硏究論文集 Vol.10 No.1
A carotenoid pigment, which was a determinant for food quality, was extracted from a marine halophilic bacteria. The stability of the pigment extract was investigated for a food additive. The optimum temperature for stability was 20℃. The pigment degradation was significantly affected by solvent polarity, however, stable in salvent methanol and ethanol. The pigment degradation was highly sensitive to light and UV exposure.