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        카드뮴 중독 흰쥐에 대한 Noninvasive Biomarker로서의 요중 Ascorbic Acid에 관한 연구

        이용우,김용호,안승주,류재두 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 1997 Journal of biomedical laboratory sciences Vol.3 No.2

        본 연구는 카드뮴의 이용 및 환경오염의 증가로 일어날 수 있는 카드뮴 중독에 대한 새로운 생물학적 지표로서의 요중 ascorbic acid를 평가하기 위하여 실험적으로 중독시킨 Sprague-Dawley종의 흰쥐를 이용하여 요중 ascorbic acid측정, 신장의 조직병리학적 조사 및 생화학적 간 기능 검사를 실시하였다. 측정된 요중 ascorbic acid농도의 전 실험기간 (50일) 평균치는 실험군Ⅰ(카드뮴 100ppm)이 214.0㎎/dl, 실험군Ⅱ(카드뮴 200ppm)가 254.3㎎/dl로 대조군 9.0㎎/dl에 비해 각각 24배 및 28배의 증가를 보였다. 조직병리학적 소견으로는 신장의 근위 세뇨관에서 단백뇨의 원인으로 추정되는 호산성 초자양 물질이 관찰되어 신장의 손상이 있었고, 생화학적 분석에서 실험군Ⅰ에서 AST, ALT의 수치가 대조군의 143㎎/dl, 50㎎/dl에 비해 각각 199㎎/dl, 88㎎/dl, 실험군Ⅱ가 270㎎/dl,226㎎/dl로 나타나 간 기능의 손상이 있었음을 알 수 있었다. 이상의 결과로 실험동물이 카드뮴에 노출됨에 따라 그 아만성 독성으로서 간장 및 신장 기능의 손상이 있었고 또한 요중의 ascorbic acid가 현저하게 증가되어 요중 ascorbic acid가 실험동물의 카드뮴 노출에 대한 noninvasive진단 지표로서 활용될 수 있을 것으로 기대된다. This study was planned to evaluate the urinary ascorbic acid as a new bilolgical marker for the intoxication of cadmium, which could possibly be driven by its increased utilization and environmental pollution. In order to meet this goal, we have performed measurement of urinary ascorbic acid concentration, histopathological examination of the kidney, and biochemical test for the liver function using cadmium-intoxicated rats by oral administration. The average concentrations of urinary ascorbic acid in the CdCl₂-treated rats were 214.0㎎/dl for 100ppm group and 254.3㎎/dl for 200ppm group during experimental period of 50 days. These levels are 24 and 28 times higher than one in the control group (9.0㎎/dl), respectively. Ultrastructural study showed the eosinophilic hyaline cast and focal effacement, fusion in the renal tubules, as well as loss of foot processes on the glomerular epithelial cells. These results suggested that cadmium may be responsible for renal glomerular injury. The blood levels of AST, ALT and LDH in the treated groups (199 IU/I, 88 IU/I, 1190 U/I for the 100 ppm group and 270 IU/I, 226 IU/I, 760 U/I for the 200 ppm group) were higher than ones in the control group(143 IU/I, 50 IU/I, 334 U/I). These results indicated the cadmium induced the damage of liver function. In conclusion, the administration of cadmium showed a remarkable increase of urinary ascorbic acid with renal and hepatic damage. Therefore, it is expected that measurement of urinary ascorbic acid would be an powerful method as a noninvasive biomarker for cadmium intoxication.

      • 제주도 연안 갈치 채낚기 어구의 생력화 : 1.연속식 채낚기어구의 모형 실험

        서두옥,정용진,김석종,이창헌,김고환,박용석 제주대학교 해양연구소 2000 해양과환경연구소 연구논문집 Vol.24 No.-

        The authors carried out a model experiment of continuoius hairtail hand line on the rooftop in order to obtain the fundamental data on elimination of labor with haritail hand line in the coast of Jeju. The results are as follow ; 1.The continuous main line was rotated smoothly by driving roller. 2.The branch lines and hooks on a main line which was rotated by rotary machine were entangled slightly. 3. The branch lines attached to rubbered model of hairtails to hooks were rotated continuously by the rotary machine.

      • 균열을 가진 강구조부재의 한계하중에 대한 유한요소해석

        박용걸,김성훈,김두환 서울産業大學校 1997 논문집 Vol.46 No.1

        The purpose of the analysis is the numerical simulation of structures strained to the limit loads. The finite element calculations and experiments with cracked structures have been carried out yielding over limit strains between 10% and 15% by single peak load. Load versus displacement-diagrams and J-diagrams up to the limit load are calculated. By this way the influence of geometric parameters may be assessed in the post yield region. It is proposed to use such calculations to correlate experiments carried out with small specimens to experiments simulating the true dimensions of the design structure.

      • 공조시스템을 적용한 석실 고분내 온ㆍ습도 분포

        전용두,이금배,박진양,고석보,전희호 公州大學校 工學硏究院 生産技術硏究所 2005 論文集 Vol.13 No.-

        Although the importance of good conservation of historic sites including ancient royal tombs is well aware, still not much attention has been paid for the facilities and systems to preserve those historic sites, which includes precious artifacts as wall paints and carved works, etc. Even the general understanding about the environment of the underground space of tombs is not satisfactory. In Korea, researchers have recently begun addressing the above issue and are making efforts to develop suitable HVAC(heating, ventilating and air-conditioning) systems for conservation of tombs. An experimental investigation was carried out to study the behavior of the HVAC system installed for a tomb. This tomb resembles in size and shape the original stone built tomb of Baek-je Dynasty, which dates back to 6th Century in southwest part of Korean peninsula. In this study, an HVAC system for a tomb(D×W×H=1.3m×3.0m×1.2m) was installed to maintain sui표 indoor conditions for conservation of tomb. The temperature and humidity inside the tomb were measured different of the setting. Finally, performance of the HVAC system is presented and discussed.

      • 수치사진측량기법을 이용한 문화재의 3차원 모델링에 관한 연구

        문두열,이봉재,신문섭,오상용 동의대학교 산업기술개발연구소 2005 産業技術硏究誌 Vol.19 No.-

        고해상도의 디지털 카메라는 근거리 수치사진측량에서 널리 유용하게 사용되고 있으며 점차 활용도가 증가하고 있다. 또한 사진측량 장비의 급속한 발전으로 정밀도가 많이 향상되었고 컴퓨터를 이용한 지형공간정보체계기술의 발달로 더욱 정밀한 3차원 지형의 재현과 면적 및 토공량 산정이 가능하게 되었다. 본 연구에서는 휴대가 간편하고 수치영상의 자체저장능력을 지닌 디지털 카메라를 이용하여 촬영조건의 다양한 변화에 따른 위치오차를 분석하였으며 광속조정을 통하여 높은 정확도의 화소 좌표를 획득하여 3차원 지형의 모니터링을 위한 기초 자료로 사용할 수 있도록 하였다. 또한 근거리수치사진측량기법의 국부지역모형생성과 문화재의 보존과 복원을 보다 효율적으로 하기 위해 수치정보 분석에 관한 연구를 수행하여 봉분의 3차원 구현에 대한 활용방안을 모색하였다. Digital high resolution cameras are widely available, and are increasingly use in digital close-range photogrammetry. And photogrammetry instruments are developing rapidly and the precision is improving continuously. The building of 3D terrains of high precision are possible and the calculation of the areas or the earthwork volumes have high precision due to the development of the technique of the spatial information system using computer. Using the digital camera which has capacity of keeping numerical value by itself and easy carrying, we analyze the positioning error according to various change of photographing condition. Also we try to find a effective method of acquiring basis data for 3D monitoring of high-accuracy in pixel degree through digital close-range photogrammetry with bundle adjustment for local terrain model generation and 3D embodiment of tumulus. In the study is about to efficient analysis of digital information data for conservation of cultural properties.

      • KCI등재

        Effect of Chlorella Diet Supplementation on Blood and Urine Cadmium Levels in Cadmium Poisoned Rats

        김용호,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.3

        This study was conducted to investigate the influence of chlorella diet supplementation. Blood accumulation and urine excretion levels were measured after the Sprague-Dawley (SD) rats were fed on a chlorella diet supplementation mixed with 40 ppm of CdCl₂. Four groups tested for blood accumulation and urine excretion levels. All four groups fed on a basic diet with a cadmium mixture. The diet for the first group contained only basic diet and the cadmium added to the drinking water. The diet for the three other groups contained cadmium to the drinking water, and 1%, 5% and 10% of chlorella added to the basic diet. A concentration of cadmium for the first group showed a 3.2±0.4 ㎍/l blood accumulatior. level and 41.5±32.9 ㎍/l urinary excretion level, and the second group, which was fed on the basic diet with 1% of chlorella added and cadmium to the drinking water showed a 1.5±0.6 ㎍/l blood level and only 14.1±1.6 ㎍/l urinary excretion level. The other two groups, which were fed on 5% and 10% of chlorella concentration and cadmium to the drinking water did not exhibit any notable effects greater than the group fed on 1% concentration of chlorella. The results suggest that the blood accumulation and urinary excretion of Cadmium are influenced by the chlorella diet supplementation from the concentration of 1% of the basic diet.

      • 배가스 폐열회수를 위한 히트파이프 적용성 검토

        전용두,박기호,고석보 公州大學校 工學硏究院 生産技術硏究所 2005 論文集 Vol.13 No.-

        A Series of experiments are performed to investigate the heat transfer characteristics of sintered metal wick heat pipe especially focusing on the operational limit of maximum allowable thermal loads and the effect of inclination slope. Tested heat pipes are composed of copper tube with pure water as a working fluid. The outer diameter of the heat pipe is 6mm and the total length is 300mm, which covers the evaporator section(50mm), adiabatic section(100mm) and condenser section(150mm). respectively. Experimental data of wall temperature distribution along the axial locations are obtained to evaluate the performance of the heat pipe at different slopes. Each test is performed by changing the input thermal loads ranging from 5W to 30W.

      • 取捨方法에 依한 竹材束數의 變化

        金榮斗 진주산업대학교 1968 論文集 Vol.2 No.-

        賈却할 통대를 檢尺할 때 寸以下 端數의 取捨方法에 따른 竹束의 變化를 要約하면 다음과 같다. 1.4捨5入, 5捨6入, 6捨7入, 7捨8入, 切捨의 5가지 取捨方法에 依하여 檢尺한 束數의 變化는 相當히 많은 增減을 보였으며, 取捨方法間에는 高度의 有意性이 認定되었다. 2.特히 切捨및 7捨8入에 依한 束數를 計算하면 많은 束數의 損失을 주게 되므로 止揚되어야 할 것이다. 3.따라서 竹材 買入時 賣却者의 立場에서는 4捨5入, 5捨6入으로 檢尺하는 것이 좋겠으나, 買入者의 立場에서는 切捨로서 檢尺하고 싶어하므로 最少限, 6捨7入에 依해서 束數를 計算해야 할 것이다. 4.束數 寸數別 本數配定은 좀 더 合理的인 方法이 講究되어서 地方差가 없도록 統一되어야 할 것이다. In selling bamboos, as they measure the bamboos acccording to the method of adoption and rejection of the less parts from ‘chi^*’, for the purpose of finding out the difference of ‘sok^**’, this experiment was accomplished as following, at the bamboo forest belonged to Chinju Agricutural & Forestry Junior Technical College in Chinju, Kyungnam Feb. 10, 1968. 1.Using the five methods of adoption and rejection; T_1, T_2, T_3, T_4, T, the difference of ‘sok’to be measured brought a great deal of add and reduce in its quantities, and 1% level of significant difference were observed between each method of adoption and rejection, 2.Especially, considering ‘sok’by the method of T_5 and T_4 these methods bring forth the big loss of ‘soks’so that it must be improved or abolished. 3.When bamboos are sold and purchased, the sellers want the method of T_1 and T_2, on the other hand, the purchasers want that of T_5, so that the method of T_3 and T_4 should be taken. 4.The (apportion of bamboos by) chiper sokhave to be taken an investigation and standarized so as not to be the difference among the region every. T_1=Counting 5 and higher fractions as units and disregarding the rest. T_2=Counting 6 and higher fractions as units and disregarding the rest. T_3=Counting 7 and higher fractions as units and disregarding the rest. T_4=Counting 8 and higher fractions as units and disregarding the rest. T_5=Cutting off the less parts from ‘chi’. chi=A units of measure in Korea.(=1.193 inches). sok=A units of counting bamboo in Korea.

      • 가이스가향나무의 揷木에 關한 硏究 : 第1報. 季節, 時期 및 揷土가 發根에 미치는 影響 1. Effect of season, date and cutting bed soil on rooting

        金榮斗 진주산업대학교 1969 論文集 Vol.3 No.-

        가이스가향나무의 效果的인 繁殖方法을 模索하기 위하여 몇가지 揷土에다 季節및 日字別로 揷木하여 그 發根成績을 調査하였는데 그 結果를 要約하면 다음과 같다. 1. 季節別로 보아 春季 및 秋季揷木이 그 成績이 우수하며, 夏季揷木은 不良하고 日字別로는 3월 15∼25일 및 9월15∼25일에 揷木한 것이 各各 우수하였다. 2. 揷土 別로 보면 春季는 赤土50%+모래50%, 秋季는 赤土70%+모래30%의 揷土에서 各各 그 成績이 우수하였다. 3. 따라서 가이스가향나무의 揷木은 봄에 있어서 3월 15∼25일에 赤土50+모래 50%에다 揷木하고 가을에는 10월 15∼25일에 赤土70%+모래30%의 揷土에다 揷木하는 것이 가장 그 發根率이 높다는 것이 認定되었으며 各處理間에는 高度의 有意性이 認定되었다. The propagation of Juniperus chinensis var, Kaizuka is difficult comparably and especially the success or failure of cutting this tree has been found among the cultivators. For the purpose of finding out these factors and more effective method of this tree, I planted stem cutting using several kinds of cutting bed soil in accordance with the season and date and investigated the result of its rooting rate. This study is the Ⅰst part of synthetic examination for cutting this tree and the results are as follows. It was recognized that the rooting rate of this tree increases that planted from March 15, to March 24, in spring using cutting bed soil of T_3(Cray50%+Sand 50%) and from October 15, to October 25. in autumn using that of T_4(Cray 70%+Sand 30%). And between each treatment significant of difference of high level was recognized.

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