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Generation of Cloned Transgenic Cats Expressing Red Fluorescence Protein1
Yin, Xi Jun,Lee, Hyo Sang,Yu, Xian Feng,Choi, Eugene,Koo, Bon Chul,Kwon, Mo Sun,Lee, Young S.,Cho, Su Jin,Jin, Guang Zhen,Kim, Lyoung Hyo,Shin, Hyoung Doo,Kim, Teoan,Kim, Nam Hyung,Kong, Il Keun Oxford University Press 2008 BIOLOGY OF REPRODUCTION Vol.78 No.3
<P>A method for engineering and producing genetically modified cats is important for generating biomedical models of human diseases. Here we describe the use of somatic cell nuclear transfer to produce cloned transgenic cats that systemically express red fluorescent protein. Immature oocytes were collected from superovulating cat ovaries. Donor fibroblasts were obtained from an ear skin biopsy of a white male Turkish Angora cat, cultured for one to two passages, and subjected to transduction with a retrovirus vector designed to transfer and express the red fluorescent protein (RFP) gene. A total of 176 RFP cloned embryos were transferred into 11 surrogate mothers (mean = 16 +/- 7.5 per recipient). Three surrogate mothers were successfully impregnated (27.3%) and delivered two liveborn and one stillborn kitten at 65 to 66 days of gestation. Analysis of nine feline-specific microsatellite loci confirmed that the cloned cats were genetically identical to the donor cat. Presence of the RFP gene in the transgenic cat genome was confirmed by PCR and Southern blot analyses. Whole-body red fluorescence was detected 60 days after birth in the liveborn transgenic (TG) cat but not in the surrogate mother cat. Red fluorescence was detected in tissue samples, including hair, muscle, brain, heart, liver, kidney, spleen, bronchus, lung, stomach, intestine, tongue, and even excrement of the stillborn TG cat. These results suggest that this nuclear transfer procedure using genetically modified somatic cells could be useful for the efficient production of transgenic cats.</P>
Xi-Jun Yin,Hyo-Sang Lee,양철주,배인휴,오동환,Seong-Gyun Cho,공일근 아세아·태평양축산학회 2007 Animal Bioscience Vol.20 No.5
This study was carried out to evaluate the effect of artificial insemination (AI) time and methods on fertilization and delivery rate at onset of estrus cats. Artificial insemination method was used by a transcervical insemination (TCI) or intra-vaginal insemination (IVI) using frozen epididymal sperm (FES) with Norwegian catheter. Semen was collected from epididymal spermatozoa after testis ectomization and frozen in Tris-buffered solution supplemented with 7% glycerol and 0.5% Orvus ES Paste. The concentration of frozen spermatozoa was adjusted to 200106 sperm/ml. The CASA data on motility and progressive motility of FES after thawing was approximately 40.35.8 and 35.96.5%. The female cats were subcutaneously treated with 50 IU pregnant mare serum gonadotropin (PMSG) and ovulated with 100 IU human chorionic gonadotropin (hCG) 4 days after PMSG injection. One treatment was inseminated at 36 h after hCG injection and the other treatment was inseminated immediately hCG injection. At 36 h after hCG injection group, 5 female cats were inseminated TCI or IVI method using 50106 sperm/0.2 ml per AI. Three of 5 female cats could be inseminated by TCI method and then delivered the 10 kittens from 2 of 3 inseminated females. Immediately hCG injection group, 4 female cats were inseminated TCI or IVI method using same sperm concentration per AI. Two of 4 female cats could be inseminated by TCI method and then delivered the 4 kittens from 1 of 2 inseminated female cats. The results revealed that AI time and method could be affected the fertilization and delivery rates at onset of estrus cats.
( Bin Yin ),( Chen Xi Hu ),( Zheng Chen ),( Wen Jun Zhao ),( Li Rong Wei ),( Yan Wen Zheng ),( Chao He ),( Yan Zeng ) 한국응용약물학회 2014 Biomolecules & Therapeutics(구 응용약물학회지) Vol.22 No.2
Vesicular Stomatitis Virus G Glycoprotein and ATRA EnhancedBystander Killing of Chemoresistant Leukemic Cells by HerpesSimplex Virus Thymidine Kinase/Ganciclovir
Liu, Xi-Jun,Lin, Xiu-Tong,Yin, Yong,Chen, Jin-Hu,Xing, Li-Gang,Yu, Jin-Ming Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.5
Objective: The aim of this study was to determine a method of dose prescription that minimizes normal tissue irradiation outside the planning target volume (PTV) during stereotactic body radiotherapy (SBRT) for patients with non-small cell lung cancer. Methods: Previous research and patients with typical T1 lung tumors with peripheral lesions in the lung were selected for analysis. A PTV and several organs at risk (OARs) were constructed for the dose calculated; six treatment plans employing intensity modulated radiotherapy (IMRT) were produced, in which the dose was prescribed to encompass the PTV, with the prescription isodose level (PIL) set at 50, 60, 70, 80, 90 or 95% of the isocenter dose. Additionally, four OARs around the PTV were constructed to evaluate the dose received in adjacent tissues. Results: The use of higher PILs for SBRT resulted in improved sparing of OARs, with the exception of the volume of lung treated with a lower dose. Conclusions: The use of lower PILs is likely to create significant inhomogeneity of the dose delivered to the target, which may be beneficial for the control of tumors with poor conformity indices.