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( Yin Shipeng ),( Hee Chul Woo ),( Jae Hyung Choi ),( Yong Beom Park ),( Byung Soo Chun ) 한국수산과학회(구 한국수산학회) 2015 Fisheries and Aquatic Sciences Vol.18 No.2
Seaweed Sargassum horneri extracts were obtained using supercritical carbon dioxide (SC-CO2) and different solvents. SC-CO2 was kept at a temperature of 45℃ and pressure of 250 bar. The flow rate of CO2 (27 g/min) was constant during the entire 2-h extraction period, and ethanol was used as a cosolvent. Six different solvents [acetone, hexane, methanol, ethanol, acetone mix methanol (7:3), and hexane mix ethanol (9:1)] were used for extraction and agitated by magnetic stirring (250 rpm) in the dark at 25℃ for 20 h; the ratio of material to solvent was 1:10 (w/v). Antioxidant properties of S. horneri extracted using SC-CO2 with ethanol and different solvents have shown good activity. The highest activity belongs to SC-CO2 with ethanol extracted oil, showing DPPH, ABTS, total phenolic content, and total flavonoid levels of 68.38 ± 1.21%, 83.51 ± 1.25%, 0.64 ± 0.02 mg/g, and 5.57 ± 0.05 mg/g, respectively. The S. horneri extracts showed a significant correlation between the antioxidant activity and phenolic content. Based on these results, the SC-CO2 extract (ethanol) of the seaweed extract from brown seaweed may be a valuable antioxidant source.
Yin, Shipeng,Woo, Hee-Chul,Choi, Jae-Hyung,Park, Yong-Beom,Chun, Byung-Soo The Korean Society of Fisheries and Aquatic Scienc 2015 Fisheries and Aquatic Sciences Vol.18 No.2
Seaweed Sargassum horneri extracts were obtained using supercritical carbon dioxide ($SC-CO_2$) and different solvents. $SC-CO_2$ was kept at a temperature of $45^{\circ}C$ and pressure of 250 bar. The flow rate of $CO_2$ (27 g/min) was constant during the entire 2-h extraction period, and ethanol was used as a cosolvent. Six different solvents [acetone, hexane, methanol, ethanol, acetone mix methanol (7:3), and hexane mix ethanol (9:1)] were used for extraction and agitated by magnetic stirring (250 rpm) in the dark at $25^{\circ}C$ for 20 h; the ratio of material to solvent was 1:10 (w/v). Antioxidant properties of S. horneri extracted using $SC-CO_2$ with ethanol and different solvents have shown good activity. The highest activity belongs to $SC-CO_2$ with ethanol extracted oil, showing DPPH, ABTS, total phenolic content, and total flavonoid levels of $68.38{\pm}1.21%$, $83.51{\pm}1.25%$, $0.64{\pm}0.02mg/g$, and $5.57{\pm}0.05mg/g$, respectively. The S. horneri extracts showed a significant correlation between the antioxidant activity and phenolic content. Based on these results, the $SC-CO_2$ extract (ethanol) of the seaweed extract from brown seaweed may be a valuable antioxidant source.