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Cheng Yi-Chi,Duarte Marcos Elias,Kim Sung Woo 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.4
Objective: The objective was to evaluate the efficacy of increasing supplementation of Yarrowia lipolytica (YL) up to 3.0% replacing 1.6% poultry fat and 0.9% blood plasma for growth performance, intestinal health and nutrient digestibility of diets fed to nursery pigs. Methods: Twenty-four pigs weaned at 24 d of age (initial body weight at 7.2±0.6 kg) were allotted to three dietary treatments (n = 8) based on the randomized complete block. The diets with supplementation of YL (0.0%, 1.5%, and 3.0%, replacing poultry fat and blood plasma up to 1.6% and 0.9%, respectively) were fed for 21 d. Feed intake and body weight were recorded at d 0, 10, and 21. Fecal score was recorded at every odd day from d 3 to 19. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure intestinal health markers including tumor necrosis factor-alpha, interleukin-8, immunoglobulin A and immunoglobulin G. Ileal digesta was collected for apparent ileal digestibility (AID) of nutrients in diets. Data were analyzed using Proc Mixed of SAS. Results: Supplementation of YL (1.5% and 3.0%) replacing poultry fat and blood plasma did not affect growth performance, fecal score and intestinal health. Supplementation of YL at 1.5% did not affect nutrient digestibility, whereas supplementation of YL at 3.0% reduced AID of dry matter (40.2% to 55.0%), gross energy (44.0% to 57.5%), crude protein (52.1% to 66.1%), and ether extract (50.8% to 66.9%) compared to diets without supplementation. Conclusion: Yarrowia lipolytica can be supplemented at 1.5% in nursery diets, replacing 0.8% poultry fat and 0.45% blood plasma without affecting growth performance, intestinal health and nutrient digestibility. Supplementation of YL at 3.0% replacing 1.6% poultry fat and 0.9% blood plasma did not affect growth performance and intestinal health, whereas nutrient digestibility was reduced. Objective: The objective was to evaluate the efficacy of increasing supplementation of Yarrowia lipolytica (YL) up to 3.0% replacing 1.6% poultry fat and 0.9% blood plasma for growth performance, intestinal health and nutrient digestibility of diets fed to nursery pigs.Methods: Twenty-four pigs weaned at 24 d of age (initial body weight at 7.2±0.6 kg) were allotted to three dietary treatments (n = 8) based on the randomized complete block. The diets with supplementation of YL (0.0%, 1.5%, and 3.0%, replacing poultry fat and blood plasma up to 1.6% and 0.9%, respectively) were fed for 21 d. Feed intake and body weight were recorded at d 0, 10, and 21. Fecal score was recorded at every odd day from d 3 to 19. Pigs were euthanized on d 21 to collect proximal and distal jejunal mucosa to measure intestinal health markers including tumor necrosis factor-alpha, interleukin-8, immunoglobulin A and immunoglobulin G. Ileal digesta was collected for apparent ileal digestibility (AID) of nutrients in diets. Data were analyzed using Proc Mixed of SAS.Results: Supplementation of YL (1.5% and 3.0%) replacing poultry fat and blood plasma did not affect growth performance, fecal score and intestinal health. Supplementation of YL at 1.5% did not affect nutrient digestibility, whereas supplementation of YL at 3.0% reduced AID of dry matter (40.2% to 55.0%), gross energy (44.0% to 57.5%), crude protein (52.1% to 66.1%), and ether extract (50.8% to 66.9%) compared to diets without supplementation.Conclusion: Yarrowia lipolytica can be supplemented at 1.5% in nursery diets, replacing 0.8% poultry fat and 0.45% blood plasma without affecting growth performance, intestinal health and nutrient digestibility. Supplementation of YL at 3.0% replacing 1.6% poultry fat and 0.9% blood plasma did not affect growth performance and intestinal health, whereas nutrient digestibility was reduced.
Bostrycin production by agro-industrial residues and its potential for food processing
Yi-Hsuan Huang,Wen-Jen Yang,Chih-Yu Cheng,Huang-Mo Sung,Shuen-Fuh Lin 한국식품과학회 2017 Food Science and Biotechnology Vol.26 No.3
Bostrycin, a red antibacterial agent produced by Nigrospora sp. no. 407, is considered for meat processing. To optimize production, the culture conditions of submerged fermentation (SmF) and solid-state fermentation (SSF) were investigated. The optimal SmF conditions were a medium containing 1.0% cane molasses and incubation at 30 C and 150 rpm for 6 days. In SSF, other than bostrycin, less pigment was produced and the optimal ratio of bagasse to water was 1:2 for 10 days. The production and recovery rate of bostrycin by SmF were 120 mg/L and 40%, respectively. Bostrycin exhibited thermostable, pHdependent color change and dose-dependent antibacterial activity against Clostridium botulinum. Bostrycin-modified meat turned strong red for at least 24 h and could not be removed by washing; bostrycin maintained its antibacterial activity with a bacteriostasis rate of 91% on Staphylcoccus aureus. This is an easy and inexpensive means of acquiring bostrycin from molasses and sugarcane.
Hypermethylation of TET1 Promoter Is a New Diagnosic Marker for Breast Cancer Metastasis
Sang, Yi,Cheng, Chun,Tang, Xiao-Feng,Zhang, Mei-Fang,Lv, Xiao-Bin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.3
Breast cancer metastasis is a major cause of cancer-related death in women. However, markers for diagnosis of breast cancer metastasis are rare. Here, we reported that TET1, a tumor suppressor gene, was downregulated and hypermethylated in highly metastatic breast cancer cell lines. Moreover, silencing of TET1 in breast cancer cells increased the migration and spreading of breast cancer cells. In breast cancer clinical samples, TET1 expression was reduced in LN metastases compared with primary tissues. Besides, the methylation level of the TET1 promoter was increased significantly in LN metastases. Taken together, these findings indicate that promoter hypermethylation may contribute to the downregulation of TET1 and could be used as a promising marker for diagnosis in patients with breast cancer metastasis.
Design and Self-Assembly of Amphiphilic Block Copolymers for Drug Delivery
Yi Yan Yang,Chuan Yang,Jeremy P. K. Tan,Nikken Wiradharma,Shrinivas Venkataraman,Amalina Bte Ebrahim Attia,Zhan Yuin Ong,Wei Cheng,Ashlynn Lee,Lin Kin Yong,Kazuki Fukushima,Sung Ho Kim,Daniel J. Coady 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.1
Wu Sing-Shan,Tseng Ching-Tzu,Yang Yu-Hung,Liu Yao-Chia,Chang Ju-Chun,Gyawali Purushottam,Li Yi-Hsuan,Yang Tzu-Hao,Tsai Yi-Fang,Tang Li-Cheng,Nai Yu-Shin 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.4
The fall armyworm, Spodoptera frugiperda, is an emerging invasive pest in Taiwan that feeds on a wide range of crops and causes serious damage. Herein, an entomopathogenic fungal library (EFLib) was constructed to identify potential microbes to control FAW. Twenty-eight indigenous entomopathogenic fungi (EPF) were iso lated and investigated for their potential pathogenicity, with Metarhizium pinghaense (Mp-NCHU-124) and Beauveria bassiana (Bb-NCHU-157) exerting dose-dependent effects on the 4th instar FAW larvae. The non-ionic surfactant Silwet L-77 rapidly killed FAW larvae after spraying at a concentration of 300 mg/kg and the toxic effect of Silwet L-77 on FAW larvae was dose-dependent. When the EPF isolates (10 6 conidia/mL) were applied to FAW larvae in combination with the non-ionic surfactant Silwet L-77 (30–90 mg/kg), the mortality rate dramatically increased and the LT 50 reduced, with increased fungal mycosis (Mp-NCHU-124: 38% to 72% and Bb-NCHU-157: 20 to 62%), indicating the high compatibility of EPF with the non-ionic surfactant. Thus, the Silwet L-77+EPF combined formulation has potential for practical field application for FAW pest control and sustainable agriculture in the future.
김무성 ( Kim Moo-sung ),박성운 ( Piao Cheng-yun ),주용이 ( Ju Yong-yi ) 한국금융공학회 2017 금융공학연구 Vol.16 No.2
본 연구는 Copula-VaR모형을 사용하여 투자자가 통화별(엔화, 위안화, 유로, 파운드 및 달러)로 KOSPI200에 투자한 경우 주가지수 변동과 환율 변동으로 인해 부담하게 되는 시장위험을 분석하였다. 2004년 1월 2일부터 2016년 10월 31일까지 주가지수와 환율의 일별 데이터를 사용하여 외국통화로 한국 주식시장에 투자한 경우, 기존의 완전가치평가방법으로 추정한 VaR와 코퓰라함수를 사용하여 추정한 VaR를 비교·분석하였다. 분석 결과를 요약하면 다음과 같다. 첫째, t 코퓰라(Student`s t-Copula)와 크레이턴 코퓰라(Clayton Copula) 및 프랭크 코퓰라(Frank Copula)를 사용한 Copula-VaR모형은 정규분포를 가정한 모형보다 금융자산 수익률의 극단상황 발생을 더 잘 나타내는 것으로 확인되었다. 둘째, 엔화의 경우 기타 통화에 비해 KOSPI200과 상대적으로 큰 양 (+)의 상관성을 보이고 있으며, VaR의 평균값이 모든 신뢰구간에서 큰 값을 나타내고 있으므로, 엔화를 사용하여 KOSPI200에 투자한 투자자가 가장 큰 위험을 부담하는 것으로 분석된다. 달러를 사용한 투자자는 가장 적은 위험을 부담하는 것으로 나타났다. This study analyzes the market risk of investors using the Copula-VaR model when investing in KOSPI 200 by currency (yen, yuan, euro, pound, and dollar) due to changes in stock index and exchange rate. We use daily data from January 2, 2004 to October 31, 2016, and compared VaR estimated using full valuation method and VaR estimated using the Copula function. The results of the analysis are summarized as follows. First, the Copula-VaR model using Student`s t-Copula, Clayton Copula and Frank Copula shows that extreme situations of financial asset returns are more likely to occur than those assuming normal distribution. It was confirmed to be better. Second, investors who invested in the KOSPI200 using yen have the greatest risk, while investors using the dollar have the lowest risk.