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- 저자 : Yasuyuki Ohta (검색결과 3 건)
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Masahide Yasuda,Yasuyuki Ishii,Kazuyoshi Ohta 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.6
Recently Napier grass (Pennisetum purpureumSchumach) has been recognized to meet the requirement oflignocellulosic bioethanol production, because it has lowlignin-content and a relatively high herbage mass per yearand per area. Therefore, pretreatment, saccharification, andfermentation processes for ethanol production from Napiergrass have been extensively studied. As pretreatmentmethod, acid, alkali, PBHW (pressurized batch hot water),and LMAA (low-moisture anhydrous ammonia) pretreatmentswere reviewed. As saccharification and fermentationprocess, saccharification followed by co-fermentation ofhexose and pentose, simultaneous saccharification andfermentation (SSF) followed by pentose fermentation,simultaneous saccharification and co-fermentation (SSCF)process were proposed. The SSCF was most advantageousprocess since the SSCF can prevent contamination risks ofother microorganism and can construct simple processingprocedure. An example of ethanol production from Napiergrass was a combination process of LMAA-pretreatmentwith SSCF which was performed for of LMAA-treatedNapier grass at 36°C for 96 h using cellulase, xylanase,Saccharomyces cerevisiae, and Escherichia coli KO11. The ethanol yield reached 74.1%. Thus, Napier grass wasthought to be a promising biomass for ethanol production.
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Antioxidant effects of citrus pomace extracts processed by super-heated steam
Wang, Lei,Jo, Min-Ji,Katagiri, Riho,Harata, Kaori,Ohta, Moemi,Ogawa, Ayane,Kamegai, Masayuki,Ishida, Yasuyuki,Tanoue, Shota,Kimura, Sojiro,Lee, Seung-Cheol,Jeon, You-Jin Elsevier 2018 FOOD SCIENCE AND TECHNOLOGY -ZURICH- Vol.90 No.-
<P>This study was performed to investigate the enhanced antioxidant effects of citrus pomace (CP) extracts processed by super-heated steam (SHS). CP extracts were obtained using a closed SHS system at three different temperatures (100, 200, and 300 degrees C) with an extraction time of 10 or 20 min. These extracts were evaluated for their antioxidant activities by measuring their DPPH, alkyl, and hydroxyl radical scavenging activity using Electron Spin Resonance (ESR) spectroscopy and for their protective effects against H2O2-induced oxidative stress in Vero cells. All extracts exhibited strong radical scavenging activities and protective effects against H2O2-induced Vero cell damage. Especially, the SHS-CP extracts obtained at 300 degrees C with extraction times of both 10 min and 20 min exhibited antioxidant activities that were significantly higher than those of ethanol extracts and SHS-CP extracts obtained at other temperatures. The total phenolic and flavonoid contents of SHS-CP extracts and their antioxidant activities were found to be increased with elevated processing temperatures. Moreover, gas chromatography/mass spectrometry (GC/MS) in the presence of a methylating reagent revealed that some characteristic polyphenols and polyhydroxycyclohexanes were present in the SHS-CP extracts. Thus, this study demonstrated that SHS extraction is an efficient and environmentally friendly method for extracting antioxidants from CP.</P>
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Jingwei Shang,Toru Yamashita,Yusuke Fukui,Dongjing Song,Xianghong Li,Yun Zhai,Yumiko Nakano,Ryuta Morihara,Nozomi Hishikawa,Yasuyuki Ohta,Koji Abe 대한신경과학회 2018 Journal of Clinical Neurology Vol.14 No.1
Background and Purpose Cognitive and cerebrovascular diseases are common in the elderly, but differences in the plasma levels and associations of plasma biomarkers in these diseases remain elusive. Methods The present study investigated differences in plasma fatty acids [eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)], adiponectin, reptin, plasma markers of inflammation [high-sensitivity C-reactive protein (hsCRP) and serum amyloid A (serum AA)], and plasma lipids [high-density lipoprotein and low-density lipoprotein (LDL)] in patients with Alzheimer’s disease (AD) (n=266), mild cognitive impairment (MCI) (n=44), vascular dementia (VaD) (n=33), and ischemic stroke (IS) (n=200) in comparison to normal controls (n=130). Results The serological data showed that lower EPA and DHA levels and higher reptin and LDL levels were associated with AD and IS, the reptin/adiponectin ratio was strongly associated with IS, the hsCRP level was more strongly associated with VaD and IS, and the serum AA level was associated with all three cognitive diseases and IS. Conclusions This is the first report of differences in the expression levels of plasma biomarkers and peripheral arterial tonometry among AD, MCI, VaD, and IS patients and normal controls. These different associations indicate that diverse pathological mechanisms underlie these diseases
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