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- 저자 : Yangyong Lv (검색결과 2 건)
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Construction of Recombinant Corynebacterium glutamicum for L-threonine Production
Yangyong Lv,Zhanhong Wu,Shuangyan Han,Ying Lin,Suiping Zheng 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
L-threonine is an essential amino acid which is widely used in feed and pharmaceutical industries. We recently engineered Corynebacterium glutamicum R102(AHVr) for improved production of L-threonine. Inactivation of genes metX and dapA encoding dihydrodipicolinate synthase and homoserine O-acetyltransferase, respectively,was firstly conducted by homologous recombination, which differed from the common random mutagenesis method. Then operon gene hom-thrB (O) and export gene thrE (E)from R102 were over-expressed alone or together to obtain a series of recombinant strains. qPCR was employed to evaluate the transcript quantification of the target genes. In flask fermentation, the newly constructed strain R102ΔmetXΔdapA (pEC-Box) was able to accumulate 3.35 g threonine/L compared with 1.80 g threonine/L of strain R102 (AHVr). L-threonine is an essential amino acid which is widely used in feed and pharmaceutical industries. We recently engineered Corynebacterium glutamicum R102(AHVr) for improved production of L-threonine. Inactivation of genes metX and dapA encoding dihydrodipicolinate synthase and homoserine O-acetyltransferase, respectively,was firstly conducted by homologous recombination, which differed from the common random mutagenesis method. Then operon gene hom-thrB (O) and export gene thrE (E)from R102 were over-expressed alone or together to obtain a series of recombinant strains. qPCR was employed to evaluate the transcript quantification of the target genes. In flask fermentation, the newly constructed strain R102ΔmetXΔdapA (pEC-Box) was able to accumulate 3.35 g threonine/L compared with 1.80 g threonine/L of strain R102 (AHVr).
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Guo Chen,Congcong Kong,Limei Yang,Mu Zhuang,Yangyong Zhang,Yong Wang,Jialei Ji,Zhiyuan Fang,Honghao Lv 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.5
Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot for cruciferous vegetables worldwide, especially for the cole crops such as cabbage and cauliflower. Due to the lack of resistant cabbage cultivars, black rot has brought about considerable yield losses in recent years in China. Understanding of the pathogen features is a key step for disease prevention, however, the pathogen diversity, population structure, and virulence are largely unknown. In this study, we studied 50 Xcc strains including 39 Xcc isolates collected from cabbage in 20 regions across China, us- ing multilocus sequence genotyping (MLST), repetitive DNA sequence-based PCR (rep-PCR), and pathogenicity tests. For MLST analysis, a total of 12 allelic profiles (AP) were generated, among which the largest AP was AP1 containing 32 strains. Further cluster analysis of rep-PCR divided all strains into 14 DNA groups, with the largest group DNA I comprising of 34 strains, most of which also belonged to AP1. Inoculation tests showed that the representative Xcc strains collected from diverse regions performed differential virulence against three brassica hosts compared with races 1 and 4. Interestingly, these results indicated that AP1/DNA I was not only the main pathotype in China, but also a novel group that differed from the previously reported type races in both genotype and virulence. To our knowledge, this is the first extensive genetic diversity survey for Xcc strains in China, which provides evidence for cabbage resistance breeding and opens the gate for further cabbage-Xcc interaction studies.
ScienceON
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