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Guo Chen,Congcong Kong,Limei Yang,Mu Zhuang,Yangyong Zhang,Yong Wang,Jialei Ji,Zhiyuan Fang,Honghao Lv 한국식물병리학회 2021 Plant Pathology Journal Vol.37 No.5
Xanthomonas campestris pv. campestris (Xcc) is the causal agent of black rot for cruciferous vegetables worldwide, especially for the cole crops such as cabbage and cauliflower. Due to the lack of resistant cabbage cultivars, black rot has brought about considerable yield losses in recent years in China. Understanding of the pathogen features is a key step for disease prevention, however, the pathogen diversity, population structure, and virulence are largely unknown. In this study, we studied 50 Xcc strains including 39 Xcc isolates collected from cabbage in 20 regions across China, us- ing multilocus sequence genotyping (MLST), repetitive DNA sequence-based PCR (rep-PCR), and pathogenicity tests. For MLST analysis, a total of 12 allelic profiles (AP) were generated, among which the largest AP was AP1 containing 32 strains. Further cluster analysis of rep-PCR divided all strains into 14 DNA groups, with the largest group DNA I comprising of 34 strains, most of which also belonged to AP1. Inoculation tests showed that the representative Xcc strains collected from diverse regions performed differential virulence against three brassica hosts compared with races 1 and 4. Interestingly, these results indicated that AP1/DNA I was not only the main pathotype in China, but also a novel group that differed from the previously reported type races in both genotype and virulence. To our knowledge, this is the first extensive genetic diversity survey for Xcc strains in China, which provides evidence for cabbage resistance breeding and opens the gate for further cabbage-Xcc interaction studies.
Structural performance of GFRP-concrete composite beams
Yang, Yong,Xue, Yicong,Zhang, Tao,Tian, Jing 국제구조공학회 2018 Structural Engineering and Mechanics, An Int'l Jou Vol.68 No.4
This paper presents the results of an experimental study on the structural performance of an innovative GFRP-concrete composite beam construction, which is reinforced with longitudinal GFRP pultruded box-profile and transverse steel stirrups. GFRP perfobond (PBL) shear connectors are employed to enhance the bonding performance between the GFRP profile and the concrete portion. To investigate the shear and flexural performance of this composite system, eight specimens were designed and tested under three-point and four-point bending. The main variables were the height of the composite beam and the shear span-to-depth ratio. The test results indicated that bonding cracks did not occur at the interface between the GFRP profile and the concrete until the final stage of the test. This shows that the specimens performed well as composite beams during the test and that the GFRP PBL connectors were reliable. Based on the test results, two calculation methods were used to determine the flexural and shear capacity of the composite beams. A comparative study of the test and theoretical results suggests that the proposed methods can reasonably predict both the flexural and shear capacities of the specimens, whereas the provisions of ACI 440 are relatively conservative on both counts.