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- 저자 : Yanfeng Shi (검색결과 4 건)
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Quanxin Gao,Yanfeng Yue,Minghua Min,Shiming Peng,Zhaohong Shi,Jinbo Wang,Tao Zhang 한국통합생물학회 2018 Animal cells and systems Vol.22 No.4
The Kelp grouper Epinephelus moara is one of the most widely consumed and economically important marine fish in China. The species can tolerate a wide range of salinity, but genomic resources are not available, and the molecular mechanisms underlying adaptation to salinity at the transcriptomic level remain largely unclear. In this study, the transcriptomic responses of the liver of E. moara under low salinity were investigated using the Illumina digital gene expression system. After de novo assembly, 499,356 transcripts were generated and contributed 445,068 unigenes. A total of 14, 19, 33 and 3101 genes were differentially expressed following exposure to low salinity stress for 2, 6, 24 and 48 h, respectively. Only two genes were differentially expressed in all groups. Four genes related to metabolism and ambient salinity adaption were randomly selected to validate the differentially expressed genes (DEGs) by real-time PCR. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to analyse the functional significance of DEGs, including those responding to salinity through diverse biological processes, cellular components, molecular functions, and pathways associated with metabolic and osmotic responses. This work provides new insight into the response to salinity challenges in E. moara, and the findings expand our knowledge of the molecular basis of metabolic regulation mechanisms in this species. Additionally, the transcriptional data provide a valuable resource for future molecular and genetic studies on E. moara.
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( Haibo Yuan ),( Yanfeng Liu ),( Xueqin Lv ),( Jianghua Li ),( Guocheng Du ),( Zhongping Shi ),( Long Liu ) 한국미생물 · 생명공학회 2018 Journal of microbiology and biotechnology Vol.28 No.12
The compound 2,5-furandicarboxylic acid (FDCA), an important bio-based monomer for the production of various polymers, can be obtained from 5-hydroxymethylfurfural (HMF). However, efficient production of FDCA from HMF via biocatalysis has not been well studied. In this study, we report the identification of key genes that are involved in FDCA synthesis and then the engineering of Raoultella ornithinolytica BF60 for biocatalytic oxidation of HMF to FDCA using its resting cells. Specifically, previously unknown candidate genes, adhP3 and alkR, which were responsible for the reduction of HMF to the undesired product 2,5- bis(hydroxymethyl)furan (HMF alcohol), were identified by transcriptomic analysis. Combinatorial deletion of these two genes resulted in 85.7% reduction in HMF alcohol formation and 23.7% improvement in FDCA production (242.0 mM). Subsequently, an aldehyde dehydrogenase, AldH, which was responsible for the oxidation of the intermediate 5-formyl-2-furoic acid (FFA) to FDCA, was identified and characterized. Finally, FDCA production was further improved by overexpressing AldH, resulting in a 96.2% yield of 264.7 mM FDCA. Importantly, the identification of these key genes not only contributes to our understanding of the FDCA synthesis pathway in R. ornithinolytica BF60 but also allows for improved FDCA production efficiency. Moreover, this work is likely to provide a valuable reference for producing other furanic chemicals.
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A SDR Prototype for Backscatter Sensor Networks
Jinrong Mo,Shengbo Hu,Feng Lv,Yanfeng Shi,Tingting Yan 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.10
Low cost and low power is one of the important reasons in the wide application of backscatter sensor networks. Based on LabVIEW and USRP platforms, this paper presents and implements a simple and configurable SDR prototype for backscatter sensor networks. The SDR prototype can apply to the multi-sensor environment with low power. The prototype consists of configurable backscatter transceiver with homodyne receiver and sensor nodes. It can reduce the power consumption of system efficiently through harvesting wireless RF energy and waking sensor nodes. The frequency division multiple access method with configurable sub-carrier is proposed, and the constraint conditions of sensing data pulse period for avoiding collision among sensors are presented and proved in this paper. The performance of the prototype is analyzed, and the closed-form solution of the probability of outage under the log-normal channel is also given and proved. Finally, the experiment and simulation show the SDR prototype is effective and feasible. The EVM value is less than 2.8%, and the probability of outage error between numerical analysis and Monte Carlo simulation is less than 1.86%.
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