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Sheng Wang,Yanbing Lv,Ruili Wu,Lin Song Li,Huaibin Shen,Ming Xing,Xia Chen 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.7
The high-quality green-emitting CdS0 . 5Se0 .5/8Zn1 − x Cd x S/2ZnS QDs with “8” and “2” monolayers (ML) of corresponding shell were first synthesized by “thermal-cycling coupled single precursor” (TC-SP) approach. The component-gradient Zn1− x Cd x S interlayer played a key role in the growth of thick shell by gradually buffering the large lattice mismatch (~9%) between the CdS0 . 5Se0 .5 core and ZnS shell. Moreover, the Zn1− x Cd x S gradient interlayer as well as ZnS outshell increased the potential barrier to prevent excitons from being trapped by surface defects. The photoluminescence quantum yields of the as-synthesized CdS0 . 5Se0 .5/8Zn1 − x Cd x S/2ZnS core/shell QDs can reach to 70% in organic media and still maintain 60% after aqueous phase transfer. The green-emitting CdS0 . 5Se0 .5/8Zn1 − x Cd x S/2ZnS core/shell QDs may be good candidates for applications of biomedical and photoelectric field.
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Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing
Defu Wang,Liyan Cui,Yanni Pei,Zhennan Ma,Shaofei Shen,Dandan Long,Lingyu Li,Yanbing Niu 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5
Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva vein cleaning virus SX strain (MVCVSX) by specific primers targeting identified contig gaps, the full-length genome sequences (9,645 nucleotides) of MVCV-SX were characterized, constituting of an open reading frame that is long enough to encode 3,096 amino acids. Phylogenetic analysis showed that MVCVSX was clustered with euphorbia ringspot virus and yam mosaic virus. Further analyses of the vsiR profiles revealed that the most abundant MVCV-vsiRs were between 21 and 22 nucleotides in length and a strong bias was found for “A” and “U” at the 5′-terminal residue. The results of polarity assessment indicated that the amount of sense strand was almost equal to that of the antisense strand in MVCV-vsiRs, and the main hot-spot region in MVCV-SX genome was found at cylindrical inclusion. In conclusion, our findings could provide new insights into the RNA silencing-mediated host defence mechanism in A. rosea infected with MVCV-SX, and offer a basis for the prevention and treatment of this virus disease.
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Yu Zheng,Keping Zhang,Guangyu Su,Qi Han,Yanbing Shen,Min Wang 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.1
The acetic acid bacterium Acetobacter pasteurianus is used for vinegar fermentation with ethanol as a substrate. However, growth of A. pasteurianus is inhibited by high ethanol concentrations. The ethanol resistance of A. pasteurianus CGMCC 3089 was improved using a continuous ethanol stress adaptation culture. Characterization studies of strains during evolutionary processes were performed for improved ethanol resistance by comparison of cell growth and alcohol dehydrogenase (ADH) and aldehyde dehydrogenases (ALDH) activities. Improved resistance against ethanol was an inheritable phenotype instead of a transient physiologic adaptation. The evolutionary response of ADH and ALDH to high concentrations of ethanol was responsible for the ethanol resistance of A. pasteurianus, instead of mutations in the open reading frames of ADH and ALDH, or long nucleotide sequence insertion or deletion in the genome.
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