http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Yakunin, A. (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
Eurasian integration and the development of Asiatic Russia
Alexander Lukin,Vladimir Yakunin 한양대학교 아태지역연구센터 2018 Journal of Eurasian Studies Vol.9 No.2
This paper argues that Russia’s strategic objective of developing its Asiatic regions is tied to its serious intentions in Asia as a whole. It stresses that Russia can only connect to the political, economic, and cultural life of Eurasia and the Asia-Pacific through its own Asian regions. Moreover, leaders’ claims that Russia belongs to both Europe and Asia will carry little weight with their Asiatic neighbors if Russia’s own Asiatic regions remain underdeveloped and subject to shrinking populations. The paper critically analyzes the results of various projects of development of Asiatic Russia beginning from late tsarist period until the 21st century and shows that Russia needs to put forward a formal strategy for developing the Eurasian infrastructure that is comparable to the SREB, Kazakhstan’s NurlyZhol (Bright Path) economic stimulus plan, Mongolia’s Steppe Road, and others. This strategy should reflect Russia’s objectives for the economic development of its own Asiatic regions, and through them, the co-development with its neighbors of Eurasia generally. It argues that the Trans-Eurasian Belt Development, put forward by several Russia think tanks, could become Russia’s contribution to the development of the Eurasian space and mesh with the Chinese, Kazakh, Mongolian, and other partner initiatives. Its implementation would help spur the economic development of Asiatic Russia, enabling that region to become part of the larger economic development of Eurasia. That would help turn Russia into a more important independent and constructive player in the Eurasian space, acting in close coordination with its partners in both the East and the West.
-
Joo, J.,Khusnutdinova, A.,Flick, R.,Kim, T.,Bornscheuer, U.,Yakunin, A.,Mahadevan, R. THE ROYAL SOCIETY OF CHEMISTRY 2017 Chemical Science Vol.8 No.2
<P>Adipic acid, a precursor for Nylon-6,6 polymer, is one of the most important commodity chemicals, which is currently produced from petroleum. The biosynthesis of adipic acid from glucose still remains challenging due to the absence of biocatalysts required for the hydrogenation of unsaturated six-carbon dicarboxylic acids to adipic acid. Here, we demonstrate the first enzymatic hydrogenation of 2-hexenedioic acid and muconic acid to adipic acid using enoate reductases (ERs). ERs can hydrogenate 2-hexenedioic acid and muconic acid producing adipic acid with a high conversion rate and yield in vivo and in vitro. Purified ERs exhibit a broad substrate spectrum including aromatic and aliphatic 2-enoates and a significant oxygen tolerance. The discovery of the hydrogenation activity of ERs contributes to an understanding of the catalytic mechanism of these poorly characterized enzymes and enables the environmentally benign biosynthesis of adipic acid and other chemicals from renewable resources.</P>
-
Exploring Bacterial Carboxylate Reductases for the Reduction of Bifunctional Carboxylic Acids
Khusnutdinova, Anna N.,Flick, Robert,Popovic, Ana,Brown, Greg,Tchigvintsev, Anatoli,Nocek, Boguslaw,Correia, Kevin,Joo, Jeong C.,Mahadevan, Radhakrishnan,Yakunin, Alexander F. Wiley Blackwell (John Wiley Sons) 2017 BIOTECHNOLOGY JOURNAL Vol.12 No.11
-
Nemr, Kayla,Mü,ller, Jonas E.N.,Joo, Jeong Chan,Gawand, Pratish,Choudhary, Ruhi,Mendonca, Burton,Lu, Shuyi,Yu, Xiuyan,Yakunin, Alexander F.,Mahadevan, Radhakrishnan Elsevier 2018 Metabolic engineering Vol.48 No.-
<P><B>Abstract</B></P> <P>Microbial processes can produce a wide range of compounds; however, producing complex and long chain hydrocarbons remains a challenge. Aldol condensation offers a direct route to synthesize these challenging chemistries and can be catalyzed by microbes using aldolases. Deoxyribose-5-phosphate aldolase (DERA) condenses aldehydes and/or ketones to β -hydroxyaldehydes, which can be further converted to value-added chemicals such as a precursor to cholesterol-lowering drugs. Here, we implement a short, aldolase-based pathway in <I>Escherichia coli</I> to produce (<I>R</I>)-1,3-BDO from glucose, an essential component of pharmaceutical products and cosmetics. First, we expressed a three step heterologous pathway from pyruvate to produce 0.3 g/L of (<I>R</I>)-1,3-BDO with a yield of 11.2 mg/g of glucose in wild-type <I>E. coli</I> K12 MG1655. We used a systems metabolic engineering approach to improve (<I>R</I>)-1,3-BDO titer and yield by: 1) identifying and reducing major by-products: ethanol, acetoin, and 2,3-butanediol; 2) increasing pathway flux through DERA to reduce accumulation of toxic acetaldehyde. We then implemented a two-stage fermentation process to improve (<I>R</I>)-1,3-BDO titer by 8-fold to 2.4 g/L and yield by 5-fold to 56 mg/g of glucose ( 11 % of maximum theoretical yield) in strain BD24, by controlling pH to 7 and higher dissolved oxygen level. Furthermore, this study highlights the potential of the aldolase chemistry to synthesize diverse products directly from renewable resources in microbes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Platform for non-natural chemicals developed using aldol condensation. </LI> <LI> Modular pathway design demonstrated in E. coli for (R)-1,3-BDO production. </LI> <LI> Carbon flux optimized by blocking pyruvate and acetaldehyde-consuming pathways. </LI> <LI> Final (R)-1,3-BDO production: 2.4 g/L and 11% of maximum theoretical yield. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
