Port-Site Metastasis of Uterine Carcinosarcoma after Laparoscopy

Zhen Tan,Ang Li,Long Chen,XiaoWen Xu,ChuanGang Fu 대한의학회 2017 Journal of Korean medical science Vol.32 No.11

We report a case of port-site metastasis after laparoscopic surgery for early stage uterine carcinosarcoma (UCS) and review the related literature. A 53-year-old woman with suspected uterine malignance underwent a total laparoscopic hysterectomy with bilateral salpingo-oophorectomy, infra-colic omentectomy, and pelvic lymphadenectomy resulting pathologically in a stage IA UCS. Twelve months later she developed a palpable abdominal-wall mass at the trocar site without other synchronous metastases. A mass resection was performed and it was pathologically diagnosed with port-site metastasis of UCS. When performing surgery for UCS, specimens should be carefully removed in case small pieces of the occult disseminated metastatic tissues are trapped between the outer surface of the trocar sleeve and the abdominal wall incisional canal. Despite the low incidence, a laparotomy might be considered rather than laparoscopy to prevent port-site metastasis and more gynecological oncology clinical practices might be relevant to the management of port-site metastasis.

A Comparison of Friction Force Calibration in Lateral Force Microscope

Wang Yuli-Ang,Kim Hong-Joon,Kong Ho-Sung,Zhao Xu-Zheng,Yoon Eui-Sung Korean Tribology Society 2006 KSTLE International Journal Vol.7 No.1

The main principle of two widely used methods which were proposed by Ruan and Bhushan, and by Ogletree and Carpick are introduced. Experiments were conducted using the two methods to measure friction force between AFM probe and silicon sample quantitatively. To characterize the frictional properties, the conversion factors of the two methods by which lateral electronic signal is converted into actual friction force were calculated. The experimental results show that that the conversion factors were extraordinarily different from each other. Further research should be done to identity the reasons for these differences.

Deduction and exploration of the evolution and function of vertebrate GFPT family

Wei Si-ang,Xu Ran,Ji Yu-yao,Ding Zhi-wen,Ding Zhi-wen 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Glutamine-fructose-6-phosphate aminotransferase (GFPT) is a key factor in the hexosamine metabolism pathway. It regulates the downstream factor O-GlcNAc to change cell function and plays an important role in the metabolism and immune process of tissues and organs. However, the evolutionary relationship of GFPT family proteins in vertebrates has not been elucidated. Objective: To deduce and explore the evolution and function of vertebrate GFPT family. Methods: 18 GFPT sequences were obtained from Homo sapiens (H. sapiens), Trachypithecus francoisi (T. francoisi), Mus musculus (M. musculus), Rattus norvegicus (R. norvegicus), Gallus gallus (G. gallus), Zootoca vivipara (Z. vivipara), Xenopus tropicalis (X. tropicalis), Danio rerio (D. rerio), Rhincodon typus (R. typus), Plasmodium relictum from National Center for Biotechnology Information (NCBI). The physical and chemical characteristics and molecular evolution of GFPT family proteins and nucleic acid sequences were analyzed by ClustalX2, Gene Doc, MEGA-X, SMART, Datamonkey, R etc. RESULTS: Based on the neighbor-joining (NJ) phylogenetic tree and evolution fingerprints, GFPT family members of vertebrates can be divided into two groups: the GFPT1 group and the GFPT2 group. Seven positive selection sites were identified by IFEL and integrated methods mixed effects model of evolution (MEME) and fixed effects likelihood (REL). Finally, we predicted 28 phosphorylation sites and 18 ubiquitousness sites in the human GFPT1 sequence, 10 phosphorylation sites, and five ubiquitousness sites in GFPT2. Gene ontology (GO) analyzes the protein molecules and KEGG signaling pathways of vertebrates interacting with GFPT family proteins. Conclusions: Our work confirmed that higher animals GFPT family may have differentiated GFPT1 and GFPT2, which meets their own functional needs. This knowledge answers the question what the origin and evolution of GFPT family in vertebrates and provided the basis for disease treatment and function research of GFPT protein.

Novel DNAH1 Mutation Loci Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Literature Review

Zhuang Bao-Jun,Xu Su-Yun,Dong Liang,Zhang Pei-Hai,Zhuang Bao-Lin,Huang Xiao-Peng,Li Guang-Sen,You Yao-Dong,Chen Di'Ang,Yu Xu-Jun,Chang De-Gui 대한남성과학회 2022 The World Journal of Men's Health Vol.40 No.4

The protein encoded by dynein axonemal heavy chain 1 (DNAH1) is a part of dynein, which regulates the function of cilia and sperm flagella. The mutant of DNAH1 causes the deletion of inner dynein arm 3 in the flagellum, leading to multiple morphological abnormalities of the sperm flagella (MMAF) and severe asthenozoospermia. However, instead of asthenozoospermia and MMAF, the result caused by the mutation of DNAH1 remains unknown. Here we report a male infertility patient with severe asthenozoospermia and teratozoospermia. We found two heterozygous mutations in DNAH1 (c.6912C>A and c.7076G>T) and which were reported to be associated with MMAF for the first time. We next collected and analyzed 65 cases of DNAH1 mutation and found that the proportion of short flagella is the largest, while the bent flagella account for the smallest, and the incidence of head deformity is not high in the sperm of these patients. Finally, we also analyzed 31 DNAH1 mutation patients who were treated with intracytoplasmic sperm injection (ICSI) and achieved beneficial outcomes. We hope our research will be helpful in the diagnosis and treatment of male infertility caused by DNAH1 mutation.

Fast Supplementary Voltage Control Strategy to Mitigate Catastrophic Trip-off Risk of Wind Turbines

Yin Lifu,Liu Yongcheng,Wang Zhuxiu,Xu Ang,Li Zhengtian,Lin Xiangning,Feng Zhongnan,Zhuo Yixin 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.6

There exists such a risk that Magnetic Control Reactor (MCR) type Static Var Compensator (SVC), which is widely used in wind farms, still outputs excessive reactive power after fault removal in the wind farm due to the slow regulating speed of MCR, leading to catastrophic trip-off problem of wind turbine. For the purpose of overcoming the shortage of MCR-type SVC on regulating speed and eff ectively controlling the transient over-voltage level of wind farm due to fault removal, an novel fast supplementary voltage control strategy for MCR-type SVC based on decision tree algorithm is proposed. The over-voltage level and trip-off risk of each wind turbine after fault removal is predicted by the proposed dynamic voltage security decision tree system. According to the predictions, appropriate capacitor banks are shed to lower the risk of wind turbine trip-off due to overvoltage, resulting from reactive power over-compensation. It can be proven with case studies that the proposed strategy is capable of reducing the risk of over-voltage in wind farms, accompanied by the excellent computational effi ciency.

Design Methodology of LLC Resonant Converters for Single-stage Power Factor Correction Application

Zhou Yufei,Liu Shuai,Ren Jiaqi,Wu Qibin,Hong Feng,Shen Ang,Xu Wei 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.5

This paper proposes a new design methodology for LLC resonant converter with single-stage power factor correction (PFC) function. By designing the achievable gain of resonant tank at a certain point of the AC voltage higher than the required gain, its gain will satisfy the requirement of the whole AC line voltage cycle and lead to a good single-stage PFC design. By adjusting switching frequency and changing voltage gain along with the AC voltage, the converter can realize PFC with high effi ciency. The proposed converter inherits the advantages of the traditional LLC resonant converter, including soft switching of power switches across the whole load range, buck-boost power conversion ability which benefi ts the optimization of the down-stream DC-DC converter. Gain characteristic and design consideration for PFC purpose are analyzed in detail. Experimental results are presented to show its validity and feasibility.

Comparative transcriptome analysis of ATP-binding cassette (ABC) transporter genes in eri-silkworm, Samia cynthia ricini in response to 1- deoxynojirimycin

Hai-Zhong Yu,Yan Ma,Shang-zhi Zhang,Dong-qiong Fei,Bing Liu,Li-ang Yang,Azharuddin Muhammad,Ming-hui Liu,Jia-Ping Xu 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

1-Deoxynojirimycin (DNJ) as a kind of alkaloid has been confirmed that could modulate glycometabolism andhas toxicity for the eri-silkworm in our previous research. On the contrary, what is the potentially defensivemechanism when the DNJ enters the eri-silkworm. Based on comparative transcriptome sequencing, we foundthat ATP-binding cassette (ABC) transporter genes could be induced significantly by DNJ. In this study, a total of16 putative ABC transporter genes were identified, which can be classified into seven subfamilies, namely oneABCA, four ABCBs, three ABCCs, two ABCDs, one ABCE, three ABCFs, and two ABCGs. Phylogenetic analysisrevealed that ScABCs had strong conservation with Bombyx mori. Reverse transcription quantitative PCR (RTqPCR)suggested that 6 ABC transporters had a strong positive correlation between RT-qPCR and transcriptomedata. Additionally, S. c. ricini ABC transporter C-subfamily 4 (ScABCC4), S. c. ricini ABC transporter G-subfamily4 (ScABCG4), S. c. ricini ABC transporter A-subfamily 3 (ScABCA3) and S. c. ricini ABC transporter C-subfamily10 (ScABCC10) showed different expression pattern in two feed dose (1% and 2% DNJ) and three time points(6h, 12 h, 48 h). This study provides the first study on identification, characterization and expression patterns ofABC transporter genes in S. c. ricini response to DNJ, and lays a foundation for further understanding of theirphysiological roles response to the alkaloid.