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High capacity ethidium bromide removal by montmorillonites
Lijuan Wang,Zhaohui Li,Xingrong Zhang,Guocheng Lv,Xisen Wang 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.12
Ethidium bromide (EtBr) is commonly used as a reagent to investigate DNA and RNA bonding in biochemistry. However, it is mutagenic and toxic; thus, its removal from the waste solution is of the top priority in lab safety practice. Although many products with high EtBr removal capacities are available on the market, developing new products with low material costs and high removal capacities is still an urgent priority. As the EtBr is in a cationic form Et+ balanced by counterion Br in aqueous solution, materials with high cation exchange capacity and large specific surface area may have great potential for efficient EtBr removal, Thus, several montmorillonites (MMTs) were evaluated for their EtBr removal capacity and methods of regeneration in this study. Results showed that both external and internal surfaces of MMTs were effective sorption sites for EtBr with a capacity up to 600mg/g. And the waste-laden materials could be regenerated or safely disposed after incineration at 500 oC for 2 h. As such, further tests on optimization and manufacturing kits or devices for practical EtBr removal in routine lab practice is of engineering priority, should MMTs be further explored as an effective material for EtBr removal.
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Optimization of acridine orange loading on 1:1 layered clay minerals for fluorescence enhancement
Wei-Teh Jiang,Yolin Tsai,Xisen Wang,Zhaohui Li 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.90 No.-
As a cationic fluorescence dye acridine orange (AO) is commonly used in biology and biochemistry for DNA analyses. In this study the light absorption and fluorescence of AO after being sorbed on kaolinite (Kao) and halloysite (Hal) were investigated. In dilute systems with initial AO concentrations of 1 × 106 to 1 ×104 M (0.3–25mmol/g loadings on Kao), both light absorption and fluorescence emission increased as the initial AO concentrations, thus, the amounts of AO sorbed increased. In contrast, the light absorption and fluorescence emission reached maxima at 1 × 105 M (3mmol/g) for Hal. In concentrated systems with initial AO concentrations of 5 × 104 –5 ×103 M (22–57 and 50–126mmol/g AO sorption on Kao and Hal), significant fluorescence quenching was observed and the fluorescence intensity decreased as the initial AO concentrations, thus, the amounts of AO sorbed increased. The results suggested that to achieve maximal fluorescence emission, monomeric AO configuration on the solid surface is a necessity. The AO sorption was mostly attributed to cation exchange between protonated AO and exchangeable cations on Kao and Hal surfaces. As such, both cation exchange capacity and specific surface area of the minerals control the maximal fluorescence emission.
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