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        Microbial Communities in Semi-consolidated Carbonate Sediments of the Southwest Indian Ridge

        Jiwei Li,Xiaotong Peng,Huaiyang Zhou,Jiangtao Li,Zhilei Sun,Shun Chen 한국미생물학회 2014 The journal of microbiology Vol.52 No.2

        White semi-consolidated carbonate sediments attached toblack ferromanganese oxide films were collected approximately50 km west of a newly discovered hydrothermal fieldnear the Southwest Indian Ridge (SWIR). The biodiversity ofthe prokaryotic communities within the field was examinedusing clone library-based culture-independent analysis ofthe exterior black oxides and the interior white carbonates. Subsequent 16S rRNA gene analysis suggested that Gammaproteobacteria,Acidobacteria, and Thaumarchaeota membersdominated the bacterial and archaeal clone libraries. To further characterize the metabolic processes within themicrobial community, analyses of the amoA (coding the alphasubunit of the ammonia monooxygenase for Archaea)and aprA (coding the alpha subunit of the dissimilatory adenosine-5 -phosphosulfate reductase for the sulfate-reducingand sulfur-oxidizing prokaryotes) functional genes wereconducted. The functional gene analysis results suggestedthat Thaumarchaeota and Alphaproteobacteria memberswere the potential players that participated in N and S cyclesin this marine carbonate sedimentary environment. Thispaper is the first to describe the microbial communities andtheir potential metabolic pathways within the semi-consolidatedcarbonate sediments of the SWIR.

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        Probabilistic and spectral modelling of dynamic wind effects of quayside container cranes

        Suning Chen,Peng Shitao,Hong Ningning,Wu Xiaotong,Chen Yunyue 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.30 No.4

        Quayside container cranes are important delivery machineries located in the most frontiers of container terminals, where strong wind attacks happen occasionally. Since the previous researches on quayside container cranes mainly focused on the mean wind load and static response characteristics, the fluctuating wind load and dynamic response characteristics require further investigations. In the present study, the aerodynamic wind loads on quayside container cranes were obtained from wind tunnel tests. The probabilistic and spectral models of the fluctuating aerodynamic loads were established. Then the joint probabilistic distributions of dynamic wind-induced responses were derived theoretically based on a series of Gaussian and independent assumption of resonant components. Finally, the results were validated by time domain analysis using wind tunnel data. It is concluded that the assumptions are acceptable. And the presented approach can estimate peak dynamic sliding force, overturning moments and leg uplifts of quayside container cranes effectively and efficiently.

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        Microbial Diversity of a Sulfide Black Smoker in Main Endeavour Hydrothermal Vent Field, Juan de Fuca Ridge

        Huaiyang Zhou,Jiangtao Li,Xiaotong Peng,Jun Meng,Fengping Wang,Yuncan Ai 한국미생물학회 2009 The journal of microbiology Vol.47 No.3

        Submarine hydrothermal vents are among the least-understood habitats on Earth but have been the intense focus of research in the past 30 years. An active hydrothermal sulfide chimney collected from the Dudley site in the Main Endeavour vent Field (MEF) of Juan de Fuca Ridge was investigated using mineralogical and molecular approaches. Mineral analysis indicated that the chimney was composed mainly of Fe-, Zn- and Cu-rich sulfides. According to phylogenetic analysis, within the Crenarchaeota, clones of the order Desulfurococcales predominated, comprising nearly 50% of archaeal clones. Euryarchaeota were composed mainly of clones belonging to Thermococcales and deep-sea hydrothermal vent Euryarchaeota (DHVE), each of which accounted for about 20% of all clones. Thermophilic or hyperthermophilic physiologies were common to the predominant archaeal groups. More than half of bacterial clones belonged to ε-Proteobacteria, which confirmed their prevalence in hydrothermal vent environments. Clones of Proteobacteria (γ-, δ-, β-), Cytophaga-Flavobacterium-Bacteroides (CFB) and Deinococcus-Thermus occurred as well. It was remarkable that methanogens and methanotrophs were not detected in our 16S rRNA gene library. Our results indicated that sulfur-related metabolism, which included sulfur-reducing activity carried out by thermophilic archaea and sulfur-oxidizing by mesophilic bacteria, was common and crucial to the vent ecosystem in Dudley hydrothermal site.

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