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Design and Implementation of Vibration Isolation System for Mobile Doppler Wind LIDAR
Xiaoquan Song,Chao Chen,Bingyi Liu,Jinbao Xia,Samo Stanič 한국광학회 2013 Current Optics and Photonics Vol.17 No.1
The operation of a Doppler wind LIDAR in a mobile environment is very sensitive to shocks and vibrations, which can cause critical failures such as misalignment of the optical path and damage to optical components. To be able to stabilize the LIDAR and to perform wind field measurements in motion, a shock absorption and vibration isolation system was designed and implemented. The performance of the vehicle-mounted Doppler wind LIDAR was tested in motion, first in a circular test route with a diameter of about 30 m and later in regular expressway traffic. The vibration isolation efficiency of the system was found to be higher than 82% in the main vibration area and shock dynamic deflection was smaller than maximal deflection of the isolator. The stability of the laser locking frequency in the same mobile environment before and after the vibration isolation system installation was also found to be greatly improved. The reliability of the vibration isolation system was confirmed by good results of the analysis of the LIDAR data, in particular the plane position indicator of the line of sight velocity and the wind profile.
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Hongjiang Han,Guoan Shen,Tianyue An,Bo Song,Suzhen Zhao,Xiaoquan Qi 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.4
Brachypodium distachyon (Brachypodium) has been developed as a model system for the temperate grasses. Hence, establishing a large insertion mutant population and identifying T-DNA insertion sites are imperative for functional genomics studies. Currently, Brachypodium has an established T-DNA collection resource, but it is far less than needed. In addition, the conventional methods for the isolation of flanking sequence tags (FSTs) characterizing the T-DNA inserts, such as TAIL-PCR, adapter ligation-mediated PCR, and inverse PCR, are time-consuming, laborious, and expensive. In this study, Agrobacterium-mediated transformation system was optimized to generate T-DNA mutants. Approximately 7000 T-DNA insertion lines were obtained. Furthermore, a simple and highly efficient method was developed to isolate T-DNA flanking sequence tags (FSTs). The procedures simplified the multi-step reaction that is required for the conventional inverse PCR and several reactions were conducted within a microscale reaction system in one tube. It is flexible to isolate FSTs for either individual or large numbers of T-DNA lines. To rapidly process the large-scale sequence data, a serial of Perl scripts were developed using the Perl Programming Language (http://www.perl.org). Using these methods, a total of 794 flanking sequences were isolated and analyzed in detail. Although the methods were developed in the process of isolating and analyzing T-DNA flanking sequences of Brachypodium, it can be applied to other plants.
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