Expression of C4.4A is a Potential Independent Prognostic Factor for Patients with Gastric Cancer

Cheng, Da-Qing,Gu, Xiao-Dong,Li, Zhen-Yang,Xiang, Jian-Bin,Chen, Zong-You Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.9

C4.4A, a metastasis-associated gene, encodes a glycolipid-anchored membrane protein which is overexpressed in several human malignancies. However, there are few data available on C4.4A expression and its relationship with progression in gastric cancer. Our study was designed to explore the expression of C4.4A in gastric cancer and to correlate it with clinical outcome. C4.4A expression was studied by quantitative real-time RT-PCR and immunohistochemistry for assessment of correlations with clinicopathological factors. C4.4A mRNA expression was significantly up-regulated in gastric cancer as compared with noncancerous tissue (p<0.05)., being observed in 107 (88.4%) of the 121 gastric cancer cases by immunohistochemistry. We found that the expression of C4.4A mRNA was correlated with size of the tumor, depth of invasion, lymph node metastasis, distant metastasis and TNM stage. Moreover, patients with overexpression of C4.4A has a significantly worse survival (p<0.05). Further multivariable analysis indicated that the expression of C4.4A was an independent prognostic indicator for gastric cancer (p<0.05). In conclusion, overexpression of C4.4A correlates with metastatic potential of gastric cancer and C4.4A could be a novel independent prognostic marker for predicting outcome.

Collaborative optimization for ring-stiffened composite pressure hull of underwater vehicle based on lamination parameters

BIN LI,Yong-jie Pang,Yan-xue Cheng,Xiao-meng Zhu 대한조선학회 2017 International Journal of Naval Architecture and Oc Vol.9 No.4

A Collaborative Optimization (CO) methodology for ring-stiffened composite material pressure hull of underwater vehicle is proposed. Structural stability and material strength are both examined. Lamination parameters of laminated plates are introduced to improve the optimization efficiency. Approximation models are established based on the Ellipsoidal Basis Function (EBF) neural network to replace the finite element analysis in layout optimizers. On the basis of a two-level optimization, the simultaneous structure material collaborative optimization for the pressure vessel is implemented. The optimal configuration of metal liner and frames and composite material is obtained with the comprehensive consideration of structure and material performances. The weight of the composite pressure hull decreases by 30.3% after optimization and the validation is carried out. Collaborative optimization based on the lamination parameters can optimize the composite pressure hull effectively, as well as provide a solution for low efficiency and non-convergence of direct optimization with design variables.

Economic performance of cable supported bridges

Bin Sun,Li-wen Zhang,Yidong Qin,Ru-cheng Xiao 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.59 No.4

A new cable-supported bridge model consisting of suspension parts, self-anchored cable-stayed parts and earth-anchored cable-stayed parts is presented. The new bridge model can be used for suspension bridges, cable-stayed bridges, cable-stayed suspension bridges, and partially earth-anchored cable-stayed bridges by varying parameters. Based on the assumption that each structural member is in either an axial compressive or tensile state, and the stress in each member is equal to the allowable stress of the material, the material quantity for each component is calculated. By introducing the unit cost of each type of material, the estimation formula for the cost of the new bridge model is developed. Numerical examples show that the results from the estimation formula agree well with that from the real projects. The span limit of cable supported bridge depends on the span-to-height ratio and the density-to-strength ratio of cables. Finally, a parametric study is illustrated aiming at the relations between three key geometrical parameters and the cost of the bridge model. The optimization of the new bridge model indicates that the self-anchored cable-stayed part is always the dominant part with the consideration of either the lowest total cost or the lowest unit cost. It is advisable to combine all three mentioned structural parts in super long span cable supported bridges to achieve the most excellent economic performance.

Protectin DX increases alveolar fluid clearance in rats with lipopolysaccharideinduced acute lung injury

Xiao-Jun Zhuo,Yu Hao,Fei Cao,Song-Fan Yan,Hui Li,Qian Wang,Bi-Huan Cheng,Bin-Yu Ying,Bin-Yu Ying,Sheng-Wei Jin 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Acute respiratory distress syndrome is a life-threatening critical syndrome resulting largely from the accumulation of and the inability to clear pulmonary edema. Protectin DX, an endogenously produced lipid mediator, is believed to exert anti-inflammatory and pro-resolution effects. Protectin DX (5 μg/kg) was injected i.v. 8 h after LPS (14 mg/kg) administration, and alveolar fluid clearance was measured in live rats (n = 8). In primary rat ATII epithelial cells, protectin DX (3.605 × 10−3 mg/l) was added to the culture medium with LPS for 6 h. Protectin DX improved alveolar fluid clearance (9.65 ± 1.60 vs. 15.85 ± 1.49, p < 0.0001) and decreased pulmonary edema and lung injury in LPSinduced lung injury in rats. Protectin DX markedly regulated alveolar fluid clearance by upregulating sodium channel and Na, K-ATPase protein expression levels in vivo and in vitro. Protectin DX also increased the activity of Na, K-ATPase and upregulated P-Akt via inhibiting Nedd4–2 in vivo. In addition, protectin DX enhanced the subcellular distribution of sodium channels and Na, K-ATPase, which were specifically localized to the apical and basal membranes of primary rat ATII cells. Furthermore, BOC-2, Rp-cAMP, and LY294002 blocked the increased alveolar fluid clearance in response to protectin DX. Protectin DX stimulates alveolar fluid clearance through a mechanism partly dependent on alveolar epithelial sodium channel and Na, K-ATPase activation via the ALX/PI3K/Nedd4–2 signaling pathway.

Structure and bioactivity of triterpenoids from the stems of Schisandra sphenanthera

Cheng-Qin Liang,Rong-Hua Luo,Ju-Ming Yan,Yan Li,Xiao-Nian Li,Yi-Ming Shi,Shan-Zhai Shang,Zhong-Hua Gao,Liu-Meng Yang,Yong-Tang Zheng,Wei-Lie Xiao,Hong-Bin Zhang,Han Dong Sun 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.2

Two new triterpenoids, schisphendilactoneA and B (1 and 2), together with three known triterpenoids,were isolated from the stems of Schisandra sphenanthera. Their structures were elucidated by spectroscopic methods,and the absolute configuration of 1 was determined bysingle-crystal X-ray diffraction. Compound 2 showedmoderate inhibitory activity against SW480 cancer cellline, and compound 5 exhibited promising anti-HIV-1activity with EC50 value of 0.52 lg ml-1 and therapeuticindex value of 117.12.

Chinese herbal injections for coronavirus disease 2019 (COVID-19): A narrative review

Xiao-Bin Zhu,Meng Guo,Zhi-Hui Zhang,Li-Hua Sun,Lei Liu,Li-Juan Zhou,Chun-Lei Shan,Yi Yang,Lian-Di Kan,Liu-Cheng Li 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.4

Background The outbreak of Coronavirus disease 2019 (COVID-19) has caused more than 180 million infections and 3.9 million deaths. To date, emerging clinical evidence has shown the synergetic benefits of Chinese herbal injections in treating this contagious respiratory disease. This review aims to summarize and analyze the efficacy and safety of Chinese herbal injections in the therapy of COVID-19. Methods The literature from 3 electronic databases, PubMed, CNKI, and Web of Science, were searched using the search terms “COVID-19”, “SARS-CoV-2”, “traditional Chinese medicine”, “herb”, “herbal”, and “injection”. Then the identified articles were comprehensively evaluated. Results Limited data demonstrated that Chinese herbal injections could significantly improve the clinical outcomes of COVID-19 patients, especially in combination with conventional treatment strategies. The benefits of which were mainly associated with the relief of symptoms, prevention of secondary infection, regulation of inflammation and immune function. There was also evidence showing the inhibitory effects on SARS-CoV-2 replication in vitro. Nevertheless, available real-world data suggested the increased risk of adverse event. Furthermore, the defects of existing researches and the insights for discovering novel antiviral drugs were prospectively discussed. Conclusion Evidence-based advances revealed that Chinese herbal injections such as XueBiJing injection and ShenMai injection, exerted potent effects against COVID-19. Further laboratory researches and clinical evaluation are needed to gather scientific evidence on the efficacy and safety.

Effect of Trichostatin A on CNE2 Nasopharyngeal Carcinoma Cells - Genome-wide DNA Methylation Alteration

Yang, Xiao-Li,Zhang, Cheng-Dong,Wu, Hua-Yu,Wu, Yong-Hu,Zhang, Yue-Ning,Qin, Meng-Bin,Wu, Hua,Liu, Xiao-Chun,Lina, Xing,Lu, Shao-Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.11

Trichostatin A (TSA) is a histone deacetylase (HDAC) inhibitor. We here investigated its effects on proliferation and apoptosis of the CNE2 carcinoma cell line, and attempted to establish genome-wide DNA methylation alteration due to differentially histone acetylation status. After cells were treated by TSA, the inhibitory rate of cell proliferation was examined with a CCK8 kit, and cell apoptosis was determined by flow cytometry. Compared to control, TSA inhibited CNE2 cell growth and induced apoptosis. Furthermore, TSA was found to induce genome-wide methylation alteration as assessed by genome-wide methylation array. Overall DNA methylation level of cells treated with TSA was higher than in controls. Function and pathway analysis revealed that many genes with methylation alteration were involved in key biological roles, such as apoptosis and cell proliferation. Three genes (DAP3, HSPB1 and CLDN) were independently confirmed by quantitative real-time PCR. Finally, we conclude that TSA inhibits CNE2 cell growth and induces apoptosis in vitro involving genome-wide DNA methylation alteration, so that it has promising application prospects in treatment of NPC in vivo. Although many unreported hypermethylated/hypomethylated genes should be further analyzed and validated, the pointers to new biomarkers and therapeutic strategies in the treatment of NPC should be stressed.

Value of Postoperative Radiation Therapy for Regional Control after Dissection in Head and Neck Squamous Cell Carcinoma Cases

Li, Xiao-Ming,Di, Bin,Shang, Yao-Dong,Tao, Zhen-Feng,Cheng, Ji-Min,He, Zhan-Guo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.7

Objective: We aimed to define clinicopathologic risk factors associated with regional recurrence (RR) and thus the effectiveness of postoperative radiotherapy (PORT) for neck control for head and neck squamous cell carcinomas (HNSCCs) with differing cervical lymph node status. Methods: A retrospective study was performed in 196 HNSCC patients with pathologically positive neck node (N+) to evaluate the high-risk factors for RR and to define the role of PORT in control after neck dissection and postoperative radiotherapy (PORT). Results: Overall, the RR rate after neck dissection and PORT was 29%. Extracapsular spread (ECS) was confirmed to be the only independent risk factor for RR. There were no significant risk factors associated with RR in the ECS- group. The 5-year disease-specific survival rate was 45%, which descended to 10% with the emergence of RR. Conclusions: ECS remains a determined risk factor for RR after neck dissection and PORT in patients with N+. PORT alone is not adequate for preventing RR in the neck with ECS after neck dissection. More intensive postoperative adjuvant therapies, especially combined chemotherapy and radiotherapy, are needed to prevent regional failure in HNSCC patients with ECS.

Collaborative optimization for ring-stiffened composite pressure hull of underwater vehicle based on lamination parameters

Li, Bin,Pang, Yong-jie,Cheng, Yan-xue,Zhu, Xiao-meng The Society of Naval Architects of Korea 2017 International Journal of Naval Architecture and Oc Vol.9 No.4

A Collaborative Optimization (CO) methodology for ring-stiffened composite material pressure hull of underwater vehicle is proposed. Structural stability and material strength are both examined. Lamination parameters of laminated plates are introduced to improve the optimization efficiency. Approximation models are established based on the Ellipsoidal Basis Function (EBF) neural network to replace the finite element analysis in layout optimizers. On the basis of a two-level optimization, the simultaneous structure material collaborative optimization for the pressure vessel is implemented. The optimal configuration of metal liner and frames and composite material is obtained with the comprehensive consideration of structure and material performances. The weight of the composite pressure hull decreases by 30.3% after optimization and the validation is carried out. Collaborative optimization based on the lamination parameters can optimize the composite pressure hull effectively, as well as provide a solution for low efficiency and non-convergence of direct optimization with design variables.

Microstructure evolution and effect on deuterium retention in oxide dispersion strengthened tungsten during He⁺ irradiation

Ding, Xiao-Yu,Xu, Qiu,Zhu, Xiao-yong,Luo, Lai-Ma,Huang, Jian-Jun,Yu, Bin,Gao, Xiang,Li, Jian-Gang,Wu, Yu-Cheng Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.12

Oxide dispersion-strengthened materials W-1wt%Pr₂O₃ and W-1wt%La₂O₃ were synthesized by wet chemical method and spark plasma sintering. The field emission scanning electron microscopy (FE-SEM) analysis, XRD and Vickers microhardness measurements were conducted to characterize the samples. The irradiations were carried out with a 5 keV helium ion beam to fluences up to 5.0 × 10²¹ ions/m² under 600 ℃ using the low-energy ion irradiation system. Transmission electron microscopy (TEM) study was performed to investigate the microstructural evolution in W-1wt%Pr₂O₃ and W-1wt%La₂O₃. At 1.0 × 10²⁰ He⁺/m², the average loops size of the W-1wt%Pr₂O₃ was 4.3 nm, much lower than W-1wt% La₂O₃ of 8.5 nm. However, helium bubbles were not observed throughout in both doped W materials. The effects of pre-irradiation with 1.0 × 10²¹ He⁺/m² on trapping of injected deuterium in doped W was studied by thermal desorption spectrometry (TDS) technique using quadrupole mass spectrometer. Compared with the samples without He⁺ pre-irradiation, deuterium (D) retention of doped W materials increased after He⁺ irradiation, whose retention was unsaturated at the damage level of 1.0 × 10²²D₂⁺/m². The present results implied that irradiation effect of He⁺ ions must be taken into account to evaluate the deuterium retention in fusion material applications.