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RISS 인기검색어
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- 저자 : Xiao Fen Hu (검색결과 3 건)
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Histomorphological study on embryogenesis of the honeybee Apis cerana
Xiao Fen Hu,Li Ke,Zhi Jiang Zeng 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.3
As important pollination species, honeybees play substantial impacts on the balance of global ecosystem, including two best-known honeybees Apis mellifera and Apis cerana. Embryogenesis is a fundamental stage of honeybee development and plays important roles in supporting the whole-life developmental process. However, few studies were reported on honeybee embryonic morphology using egg section, possibly due to the fragility of honeybee eggs and the difficulty of making embryonic sections. In this study, we reported a simply equipped method of frozen sectioning and PI (propidium iodide) staining to show the inner structure and cell distribution of A. cerena embryos at the different embryonic developmental stages. We found that the stages of A. cerena embryogenesis could also be typically classified into ten developmental stages, which are similar with the sister honeybee species, A. mellifera. To be noted, besides the cell distribution in the whole egg, we clearly observed the migration route of embryonic cells during the early embryonic development in A. cerena. This study provides a new insight into the whole process of honeybee embryogenesis from the perspective of egg sectioning, a histological basis for genetic manipulation using A. cerena eggs, and a reference method for egg sectioning for other insect species.
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Degradation of Lignocelluloses in Rice Straw by BMC-9, a Composite Microbial System
( Hongyan Zhao ),( Hai Ru Yu ),( Xu Feng Yuan ),( Ren Zhe Piao ),( Hu Lin Li ),( Xiao Fen Wang ),( Zong Jun Cui ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.5
To evaluate the potential utility of pretreatment of raw biomass with a complex microbial system, we investigated the degradation of rice straw by BMC-9, a lignocellulose decomposition strain obtained from a biogas slurry compost environment. The degradation characteristics and corresponding changes in the bacterial community were assessed. The results showed that rapid degradation occurred from day 0 to day 9, with a peak total biomass bacterium concentration of 3.3 × 10(8) copies/ml on day 1. The pH of the fermentation broth declined initially and then increased, and the mass of rice straw decreased steadily. The highest concentrations of volatile fatty acid contents (0.291 mg/l lactic acid, 0.31 mg/l formic acid, 1.93 mg/l acetic acid, and 0.73 mg/l propionic acid) as well as the highest xylanse activity (1.79 U/ml) and carboxymethyl cellulase activity (0.37 U/ml) occurred on day 9. The greatest diversity among the microbial community also occurred on day 9, with the presence of bacteria belonging to Clostridium sp., Bacillus sp., and Geobacillus sp. Together, our results indicate that BMC-9 has a strong ability to rapidly degrade the lignocelluloses of rice straw under relatively inexpensive conditions, and the optimum fermentation time is 9 days.
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New Triterpenoids from the Fruits of Schisandra wilsoniana and Their Biological Activities
Gao, Xue-Mei,Li, Yun-Qi,Shu, Li-Dan,Shen, Yan-Qiong,Yang, Li-Ying,Yang, Liu-Meng,Zheng, Yong-Tang,Sun, Han-Dong,Xiao, Wei-Lie,Hu, Qiu-Fen Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.3
Investigation of an organic extract of the fruits Schisandra wilsoniana led to the isolation of two new highly oxygenated nortriterpenoids, named schilancidilactones V-W (1-2). Their structures were elucidated by spectroscopic evidence. Compounds 1-2 feature a double bond between C-7 and C-8 compared with related known nortriterpenoids isolated from the genus Schisandra. Compounds 1 and 2 were tested for their anti-HIV-1 activities and cytotoxicity. The results revealed that compounds 1 and 2 showed moderate anti-HIV-1 activities with $EC_{50}$ 3.05 and 2.87 ${\mu}g/mL$, respectively, and compound 1 showed high cytotoxicity against KB and MDA-MB-231 cell with $IC_{50}$ values of 3.18 and 5.22 ${\mu}M$, respectively.
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