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        Experimental Study on the Bend and Shear Behaviors of Steel-concrete Composite Beams with Notched Web of Inverted T-shaped Steel Section

        Guo-Qiang Li,Xianhui Li,Liang Li 한국강구조학회 2012 International Journal of Steel Structures Vol.12 No.3

        A new steel-concrete composite beam with notched web of inverted T-shaped steel section is proposed in this paper. This composite beam is composed of concrete slab and inverted T-shaped steel beam. Experimental studies on the bend and shear behavior of the composite beam are conducted. Four specimens are tested to investigate the bend behavior of the novel composite beam, and the formula for predicting the bend capacity is proposed and verified by comparing the results between predicted with the formula and the measured from the tests. Six specimens are also tested to study the shear behavior and the formula for estimating the shear capacity of the novel composite beam is recommended on the base of the test results. The formulas proposed in this paper may be employed to estimate the bend and shear capacity of the composite beam with notched web of inverted T-shaped steel section for application in practice.

      • KCI등재

        Cloning and Characterization of the LFY Homologue from Chinese Cabbage (Brassica rapa subsp. pekinensis)

        Xianhui Qi,Brad Townsley,José Antonio Aguilar-Martínez,Lihui Yin,Xingying Gao,Leiping Hou,Meiying Gao,Meilan Li 한국원예학회 2015 Horticulture, Environment, and Biotechnology Vol.56 No.6

        Flowering is critical to the growth and development of plants, and LFY gene homologues play a major role in flowering initiation. To understand the genetic and molecular mechanisms underlying floral initiation and development in Brassica rapa subsp. pekinensis, BrpLFY, a homologue of LFY, was cloned using RT-PCR. Sequence analysis showed that the cDNA sequence of BrpLFY is 1,341 bp in length, with an ORF of 1,245 bp encoding a predicted protein of 415 amino acids. The predicted protein showed a high degree of identity with LFY homologues from other angiosperm species. Real-time PCR analysis showed that BrpLFY mRNA was detected in all tissues during plant development from the vegetative state to fully differentiated flowers, and its expression was highest in the cotyledon and lowest in the root. BrpLFY expression in the shoot apex increased gradually during vegetative growth and increased dramatically at stage 1 of flower bud differentiation. The relative expression peaked at stage 5 and then decreased in later stages. Moreover, the trend in BrpLFY expression level change in the shoot apex was similar regardless of variety or vernalization method. The relative expression of BrpLFY in leaves gradually decreased with leaf development. We overexpressed the gene in Arabidopsis thaliana using the floral dip method, and examined flowering time in wild-type and transgenic plants. Overexpression of BrpLFY specifically caused early flowering; the transgenic plants flowered 10-14 d earlier than did wild-type plants, and leaf number decreased by 0.5-1 when the plants bolted. Real-time PCR analysis showed that the expression of BrpLFY in transgenic Arabidopsis was higher than in wild-type plants. These results indicate that BrpLFY plays a role in promoting flowering in Chinese cabbage.

      • KCI등재

        Solidification Structure and Segregation of High Magnetic Induction Grain-Oriented Silicon Steel

        Xin Li,Min Wang,YanPing Bao,Jian Gong,Xianhui Wang,Pang Weiguang 대한금속·재료학회 2019 METALS AND MATERIALS International Vol.25 No.6

        The solidification structure and centerline segregation of high magnetic induction grain-oriented silicon steel slabs werestudied to describe the characteristics of solidification and compare the degree of centerline segregation of continuouslycast slabs. Industrial experiments were conducted to investigate the solidification behavior of slabs by secondary coolingsegment electromagnetic stirring. Three typical slabs were produced by S-EMS with current intensities of 0, 200, and 350 A. Molten steel cast at a low stirring intensity (0 A) resulted in a coarse structure relative to those cast at higher stirring intensities(200 and 350 A). The centerline segregation of carbon and silicon markedly increased with increases in S-EMS currentintensity. Composition distribution by electron probe microanalysis identified segregation spots as the sources of centerlinesegregation. Experimental results indicate that to optimize the centerline segregation of grain-oriented silicon steel slabs,the columnar crystal zone should be enlarged and the equiaxed crystal zone be reduced.

      • KCI등재

        Spatial protein expression of Panax ginseng by in-depth proteomic analysis for ginsenoside biosynthesis and transportation

        Xiaoying Li,Xianhui Cheng,Baosheng Liao,Jiang Xu,Xu Han,Jinbo Zhang,Zhiwei Lin,Lianghai Hu 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.1

        Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

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