Laser Raman detection of platelet as a non‐invasive approach for early and differential diagnosis of Alzheimer's disease

Chen, P.,Tian, Q.,Baek, S.J.,Shang, X.L.,Park, A.,Liu, Z.C.,Yao, X.Q.,Wang, J.Z.,Wang, X.H.,Cheng, Y.,Peng, J.,Shen, A.G.,Hu, J.M. WILEY‐VCH Verlag 2011 Laser physics letters Vol.8 No.7

<P><B>Abstract</B></P><P>Early and differential diagnosis of Alzheimer's disease (AD) is a problem that puzzled many doctors. Reliable markers in easy‐assembling samples are of considerable clinical diagnostic value. In this work, laser Raman spectroscopy (LRS) was developed a new method that potentially allows early and differential diagnosis of AD from the platelet sample. Raman spectra of platelets isolated from different ages of AD transgenic mice and non‐transgenic controls were collected and analyzed. Multilayer perceptron networks (MLP) classification method was used to classify spectra and establish the diagnostic models. For differential diagnosis, spectra of platelets isolated from AD, Parkinson’s disease (PD) and vascular dementia (VD) mice were also discriminated. Two notable spectral differences at 740 and 1654 cm<SUP>–1</SUP> were revealed in the mean spectrum of platelets isolated from AD transgenic mice and the controls. MLP displayed a powerful ability in the classifying of early, advanced AD and the control group, and in differential diagnosis of PD and advanced AD, as well as VD and advanced AD. The results suggest that platelet detecting by LRS coupled with MLP analysis appears to be an easy and accurate method for early and differential diagnosis of AD. This technique could be rapidly promoted from laboratory to the hospital. (© 2011 by Astro Ltd., Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA) (© 2011 by Astro Ltd., Published exclusively by WILEY‐VCH Verlag GmbH & Co. KGaA)</P>

Enhancing dendritic cell vaccine potency by combining a <i>BAK/BAX</i> siRNA‐mediated antiapoptotic strategy to prolong dendritic cell life with an intracellular strategy to target antigen to lysosomal compartments

Kang, Tae Heung,Lee, Jin Hyup,Noh, Kyung Hee,Han, Hee Dong,Shin, Byung Cheol,Choi, Eun Young,Peng, Shiwen,Hung, Chien&#x2010,Fu,Wu, T.&#x2010,C.,Kim, Tae Woo Alan R. Liss, Inc 2007 International journal of cancer Vol.120 No.8

<P><B>Abstract</B></P><P>Dendritic cell (DC)‐based vaccines have become important in immunotherapeutics as a measure for generating antitumor immune responses. We have previously demonstrated that linkage of the antigen gene to a lysosomal targeting signal, a sorting signal of the lysosome‐associated membrane protein type 1 (LAMP‐1), enhances the potency of DC‐based vaccines. DCs have a limited life span, hindering their long‐term ability to prime antigen‐specific T cells. In this study, we attempted to further improve the potency of a DC vaccine that targets human papilloma virus 16 (HPV16) E7 to a lysosomal compartment (DC‐Sig/E7/LAMP‐1) by combining a strategy to prolong DC life. We show that small interfering RNA‐targeting Bak and Bax proteins can be used to allow transfected DCs to resist being killed by T cells. This is done by downregulating these proapoptotic proteins, which have been known as so‐called gate keepers in mitochondria‐mediated apoptosis. DCs expressing intact E7 or Sig/E7/LAMP‐1 became resistant to attack by CD8<SUP>+</SUP> T cells after transfection with <I>BAK/BAX</I> siRNA, leading to enhanced E7‐specific T cell activation <I>in vitro</I> and <I>in vivo</I>. More importantly, vaccination with E7‐presenting DCs transfected with <I>BAK/BAX</I> siRNA generated a strong therapeutic effect against an E7‐expressing tumor in vaccinated mice, compared with DCs transfected with control siRNA. Our data indicate that a combination of strategies to enhance intracellular Ag processing and to prolong DC life may offer a promising strategy for improving DC vaccine potency. © 2007 Wiley‐Liss, Inc.</P>

Microwave Absorption and Magnetic Properties of Cobalt Ferrites/Carbon Nanotubes Nanocomposites

B. B. Zhang,P. F. Wang,J. C. Xu,Y. B. Han,H. X. Jin,D. F. Jin,X. L. Peng,B. Hong,J. Li,J. Gong,H. L. Ge,Z. W. Zhu,X. Q. Wang 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.5

Owing to the unique microstructure and the excellent dielectric properties, carbon nanotubes(CNTs) were decorated with CoFe2O4 nanoparticles to synthesize the CoFe2O4/CNTs nanocomposites by the solvothermal method. The phase structure, morphology, magnetic properties and microwave absorption performance of the as-prepared CoFe2O4/CNTs were characterized and discussed by X-ray diffraction (XRD), thermal gravity analysis (TGA), transmission electron microscope (TEM), vibrating sample magnetometer (VSM) and vector network analyzer (VNA). All results indicated that the diameter of CoFe2O4 nanoparticles decorating on the surface of CNTs increased with the solvothermal temperature. CoFe2O4/CNTs prepared at 180℃, 200℃ and 220℃ exhibited superparamagnetism, while the other samples presented ferromagnetism at room temperature. And with the increasing solvothermal temperature, the saturation magnetization and coercivity increased up to 72 emu/g and 2000 Oe for the sample prepared at 260℃ (S-26). And the reflection loss of CoFe2O4/CNTs nanocomposites increased with the solvothermal temperature up to -15.7 dB for S-26 with the bandwidth of 2.5 GHz.

Nanowire Perovskite Solar Cell

Im, Jeong-Hyeok,Luo, Jingshan,Franckevic&#x30c,ius, Marius,Pellet, Norman,Gao, Peng,Moehl, Thomas,Zakeeruddin, Shaik Mohammed,Nazeeruddin, Mohammad Khaja,Gra&#x308,tzel, Michael,Park, Nam-Gyu American Chemical Society 2015 NANO LETTERS Vol.15 No.3

<P>Organolead iodide perovskite, CH<SUB>3</SUB>NH<SUB>3</SUB>PbI<SUB>3</SUB>, was prepared in the form of nanowire by means of a small quantity of aprotic solvent in two-step spin-coating procedure. One-dimensional nanowire perovskite with the mean diameter of 100 nm showed faster carrier separation in the presence of hole transporting layer and higher lateral conductivity than the three-dimensional nanocuboid crystal. Reduction in dimensionality resulted in the hypsochromic shift of both absorption and fluorescence spectra, indicative of more localized exciton states in nanowires. The best performing device employing nanowire CH<SUB>3</SUB>NH<SUB>3</SUB>PbI<SUB>3</SUB> delivered photocurrent density of 19.12 mA/cm<SUP>2</SUP>, voltage of 1.052 V, and fill factor of 0.721, leading to a power conversion efficiency (PCE) of 14.71% at standard AM 1.5G solar illumination. A small <I>I</I>–<I>V</I> hysteresis was observed, where a PCE at forward scan was measured to be 85% of the PCE at reverse scan.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/nalefd/2015/nalefd.2015.15.issue-3/acs.nanolett.5b00046/production/images/medium/nl-2015-00046q_0009.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/nl5b00046'>ACS Electronic Supporting Info</A></P>

CATALYTIC SYNTHESIS AND GROWTH MECHANISM OF TUNGSTEN NANOWIRE ARRAYS ON SiO2 SUBSTRATES

C. WANG,Y. H. HE,L. Z. HOU,S. L. WANG,X. L. LIU,Q. ZHANG,C. Q. PENG 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.1

Single-crystalline W nanowires, with approximately 150 nm in diameter and 15 μm in length, have been successfully synthesized on SiO2 substrates by chemical vapor deposition (CVD) with the assistance of Ni catalysts at 950°C. The catalysts located at the tip of W nanowires were found to be Ni4W in a solid state, rather than a liquid state. The low-temperature growth of W nanowires using the solid catalysts can be generally accessible, provided that the appropriate combination of solid catalysts and nanowires are thermodynamically available, thus suggesting the implication for the potentially large-area integrated growth on various substrates. The growth direction of the generated [100]-oriented W nanowires was presumed to be determined by the orientation relationship between the solid Ni catalyst particle and the W precipitate. A possible catalytic growth model was proposed according to the analysis of the experimental results. The orientation relationship between the solid catalyst particle and the corresponding nanowire was expected to be also valid for some other nanowires induced by solid catalyst.

Tailoring the Impact Toughness of Sintered NdFeB Magnets via Surface Coating

X. G. Cui,J. X. Pan,C. Y. Cui,P. Mei,X. H. Wang,C. Fang,T. Y. Ma,C. Wang,X. C. Peng 한국자기학회 2018 Journal of Magnetics Vol.23 No.1

Metallic coating by electroplating is commonly attractive for improving the corrosion resistance of sintered NdFeB magnets. However, its tailoring of mechanical characteristics for sintered NdFeB magnets has been seldom concerned. Herein, the impact toughnesses of sintered NdFeB magnets with various metallic coatings (Ni or Ni/Sn) were comparatively investigated. The results indicate that the impact toughnesses of sintered NdFeB magnets are both improved by Ni coating and Ni/Sn bilayer coating. And Ni/Sn bilayer coating exhibits more enhancement of the impact toughness, increased by 41.6% compared with the original magnet. Moreover, the microstructural observations of the metallic coatings and the fracture were conducted, and the enhanced mechanism of impact toughness for the magnet is analyzed. These findings may provide a reference for toughening the brittle materials.

Quantitative Trait Loci Mapping for Porcine Backfat Thickness

Wu, X.L.,Lee, C.,Jiang, J.,Peng, Y.L.,Yan, H.F.,Yang, S.L.,Xiao, B.N.,Liu, X.C.,Shi, Q.S. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.7

A partial genome scan using porcine microsatellites was carried out to detect quantitative trait loci (QTL) for backfat thickness (BFT) in a pig reference population. This population carried QTL on chromosomes 1, 13 and 18. The QTL on chromosome 1 was located between marker loci S0113 and SW1301. The QTL corresponded to very low density lipoprotein receptor gene (VLDLR) in location and in biological effects, suggesting that VLDLR might be a candidate gene. The QTL found on chromosome 13 was found between marker loci SWR1941 and SW864, but significance for the marker-trait association was inconsistent by using data with different generations. The QTL on chromosome 18 was discovered between markers S0062 and S0117, and it was in proximity of the regions where IGFBP3 and GHRHR were located. The porcine obese gene might be also a candidate gene for the QTL on chromosome 18. In order to understand genetic architecture of BFT better, fine mapping and positional comparative candidate gene analyses are necessary.

Mapping a Quantitative Trait Locus for Growth and Backfat on Porcine Chromosome 18

Wu, X.L.,Lee, C.,Jiang, J.,Peng, Y.L.,Yang, S.L.,Xiao, B.N.,Liu, X.C.,Shi, Q.S. Asian Australasian Association of Animal Productio 2001 Animal Bioscience Vol.14 No.12

A QTL was localized near S0120 on porcine chromosome 18. The QTL was significant (p<0.05) for average daily gain (ADG) of body weight and backfat thickness (BFT). The estimates of additive and dominance effects for the QTL were 0.0135 kg/day (p<0.001) and 0.0138 kg/day (p>0.5) for ADG and 1.6115 mm (p<0.001) and 0.9281 mm (p>0.05) for BFT. The location of this QTL coincided with a few growth hormone pathway genes. This study suggested that a QTL allele probably resulted from a mutation responsible for physiological lipase deficiency favoring obesity. This QTL might be important to obesity as well as growth in pigs.

Study on Thermal Conductivity and Interface Bonding Properties of Polyetheretherketone/Zinc-aluminum Alloy Composites

Y. Peng,C. G. Long,X. Peng,Q. C. Liu 한국고분자학회 2021 폴리머 Vol.45 No.3

In order to improve the thermal conductivity of polyetheretherketone (PEEK), the ZA8 zinc aluminum alloy powder was used as a thermal conductive filler. PEEK / ZA8 thermal composites were prepared by the molding process. The effects of surface modification and ZA8 contents on the thermal conductivity and mechanical properties of PEEK / ZA8 composites were investigated. Meanwhile, the first-principle calculation method based on density functional theory was used to study the surface adsorption characteristics and interface bonding mechanism of the PEEK / ZA8. Results show that the thermal conductivity of the composites is improved after ZA8 surface modification, but the mechanical properties of the composites decreased with the increase of ZA8 content. When the ZA8 content is 40%, the thermal conductivity of the composites is 0.298 Wm<SUP>-1</SUP>K<SUP>-1</SUP>, which is 72.25% higher than that of pure PEEK. On this basis, 5 wt% graphite is added, the thermal conductivity is 0.418 Wm<SUP>-1</SUP>K<SUP>-1</SUP>, which is 40.27% higher than that of PEEK / ZA8 without graphite, and 141.62% higher than that of pure PEEK. The first-principle calculation results show that the existence of hydroxyl group can change the adsorption properties of aluminate on Zn (001) surface. From physical adsorption to chemical adsorption, the bond strength between aluminate and Zn surface is greatly improved.

A novel lifetime-based phosphor thermography using three-gate scheme and a low frame-rate camera

Cai, T.,Peng, D.,Liu, Y.Z.,Zhao, X.F.,Kim, K.C. Elsevier 2017 Experimental thermal and fluid science Vol.80 No.-

<P>Lifetime-based method using multiple-frame technique and sophisticated high-speed cameras has been developed for two-dimensional phosphor thermography in recent years, which is however limited due to high cost and long time in data processing. In this study, a novel lifetime-based method using three-gate scheme and a low frame-rate camera was proposed, which is able to significantly reduce equipment cost, simplify data processing, and remarkably increase spatial resolution. Here, Mg4FGeO6:Mn was used as sensor material, which was excited by a pulsed UV LED. A low frame-rate CCD camera at 4 Hz was sufficient to conduct phosphor thermometry using a three-gate scheme: one in fully excited region, one in decay region and one for background subtraction. Calibration was performed with a temperature range from 200 degrees C to 550 degrees C, and the results agreed well with data obtained by a high-speed camera sampling at 40 kHz. The effect of exposure time was investigated to yield an optimum value of 2 ms in this study. Finally, this new method was demonstrated in temperature measurements of the steady and impulsively started impingement jet heat transfer. (C) 2016 Elsevier Inc. All rights reserved.</P>