韓國産 자작나무科의 系統分類學的 硏究(序論)

李相泰,蘇雄永,田寬培,李愚喆 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

The present paper is an introduction to the whole project of "The systematic studies on Korean Betulaceae". Three studies under the project were performed this year and presented in this report : a review of taxonomic literatures and the construction of distribution maps by Woo-Tchul Lee, pollen morphology by Sang-Tae Lee and Moo-Yeol Kim, and wood anatomy by Woong-Young Soh and Kwan-Bae Jeon.

韓國産 裸予植物에 대한 系統分類學的인 硏究(槪要)

蘇雄永,宣炳崙,李相泰,朴相珍 全北大學校 生物學硏究所 1983 生物學硏究年報 Vol.4 No.-

This is the introductory paper of Systematic Studies of the Korean Gynmosperms. To elucidate the relationships among them, three parts works were performed ; external morphology, wood anatomy, and pollen morphology. As the results, three parts works were well consisted with the seperation of the Genus Pinus into two Subenus, Haploxylon and Diploxylon. And wood anatomical features strongly indicated that the Genus Cephalotaxus which was treated as a seperate family by some authors (Engler, 1926 ; Nakai, 1952 ; Cronquist et al., 1966) was different from the Genus Taxus and Torreya.

자작나무科의 導管形態에 依한 分類

蘇雄永,田寬培 全北大學校 生物學硏究所 1981 生物學硏究年報 Vol.2 No.-

To determine the degrees of specialization and intergeneric relationship within the Korean Betulaceae a comparative anatomy was performed with 3-4 year-old parts of growth rings of the stem wood from a total of 18 species and 7 varieties of the family. Some specialized anatomical characters suggests the family to be more or less advanced. Tribe Betuleae possessing scalariform perforation plates exclusively, steeper angle of end wall and alternate pittings, was thought to be more primitive than tribe Coryleae. Among the Betuleae, Alnus exhibited to be more primitive than Betula on the bases of the number of bars on the perforation plate, the angle of end wall and the pitting pattern. Among the coryleae, it was thought that Corylus having a scalariform perforation plate exclusively, and a intermediate number of bars was the most primitive, whereas both Carpinus and Ostrya having simple perforation plates and homogeneous I ray were the most advanced.

참죽나무(Cedrela sinensis Juss)의 培養細胞로 부터 體細胞胚 形成과 人工種子 生産

蘇雄永,呂邑東,蘇祥燮 全北大學校 基礎科學硏究所 1991 基礎科學 Vol.14 No.2

참죽나무의 배양세포를 물리 및 화학적인 복합방법으로 동조화를 거쳐 균일한 체세포배형성을 시도하였던바 85%의 높은 향상율을 보였다. 이에 다시 몇가지 아미노산, ABA 및 저온처리를 하여 얻은 정상적인 체세포배를 인공종자로 만들었다. 아미노산 중에서 aspartic acid 및 asparagine의 처리는 정상체세포배형성에 효과적이었는데 인공종자발아에는 별로 성과를 나타내지 않았다. ABA 및 저온처리로 정상체세포배형성율을 향상시킬 수 있었으나 인공종자의 발아에는 저온처리를 한 경우가 더욱 효과적이었다. 그러므로 간단하게 저온처리를 하여 경화된 건실한 체세포배 및 인공종자를 얻을 수 있고 이로 부터 클론식물의 대량생산이 가능할 것으로 사료된다. Somatic embryogenesis in cell suspension cultures of Cedrela sinensis Juss. was synchronized by physical and chemical treatments. Using culture medium containing aspartic acid and asparagine of 5mM each, the quality of somatic embryo from synchronized cell clumps was enhanced, but it was not effective for artificial seed germination. Somatic embryos were singly encapsulated with alginic acid to be used as an artificial seed. To improve the germination rate of artificial seeds, somatic embryos were hardened by ABA and low temperature(4℃) treatments before encapsulation. Low temperature treatment was more effective than ABA treatment.

자작나무科 植物 뿌리의 木部解剖에 依한 系統學的 硏究

蘇雄永,李昌福 全北大學校 生物學硏究所 1982 生物學硏究年報 Vol.3 No.-

A comparative root antomy of the Betulaceae in Korea including 16 species of 5 genera has been performed to elucidate the intergeneric relationship within the family. From anatomical description of the family it appeares that the root xylem of the Betulaceae possesses an array of unspecialized characters in such features as scalariform perforation plates, steeper angle of end walls and opposite pittings and it also possesses a series of specialized characters showing simple perforation plates, less steep end walls, and homogenous I ray. It is considered that the series of specialization in the family is as follows ; Alnus→Betula→Corylus→Carpinus→Ostrya. Alnus possesses many-barred perforation plates, however, Betula has an intermediate number of bars in the perforation plates. In Corylus, the perforation plates which are scalariform have a few or intermediate member ofbars. Carpinus shows a tendency toward simple perforations, and the perforation plates are entirely simple in Ostrya.

韓國産 裸予植物에 대한 系統分類學的 硏究 : 소나무科의 木村解剖 Wood Anatomy of the Pinaceae

蘇雄永 全北大學校 生物學硏究所 1983 生物學硏究年報 Vol.4 No.-

Anatomical features and dimension of tracheid and ray parenchyma of secondry xylem in the trunk of the family Pinaceae including 6 genera and 21 species and 1 variety grown in Korea were studied systematically. ⑴ Among species or genera of the family studied, diagnosis by the dimension of tracheid is usually indistinct but sometimes distinct and diagnosis by the height of ray is more or less distinct. ⑵ The genus Abies is distinguished from other genera studied by the absence of distinct ray tracheid. In the genus, resin canal is not observed, bordered pit on the axial tracheid is arranged in 1-2 rows, scalloped torus margin is rarely observed, many simple pits and nodular thickening are distributed on the vertical wall of ray cells, and the type of cross-field pitting is taxodioid. ⑶ The genus T suga differs from the genus Cedrus by the presence of smooth torus margin on the axial tracheid, and from the genus Abies by the presence of ray tracheid. The type of cross-field pitting is mainly piceoid but often taxodioid. ⑷ The genus Cedrus has the most clear scalloping of the tours margin of the bordered pit. In this genus, resin canl is absent, ray tracheid is present, and the cross-field pitting is piceoid, but sometime taxodioid. ⑸ The genus Larix has resin canal and has cupressoid or piceoid cross-field pitting. Bordered pit of the axial tracheid is arranged in 1-2 rows. Horizontal wall thickening of ray tracheid is smooth in it. ⑹ The genus Picea is distinct from the other genera studied on account of possessing of distinct piceoid cross-field pitting. The occurrence of smooth or minute dentaions on the ray tracheid walls is frequently observable. Bordered pit on the axial tracheid wall is arranged in one row. Thick walled resin canal is present. ⑺ The genus Pinus is readily distinguishable from all other genera by the presence of resin canal with thin walled epithelial cells. The genus is subdivided into the subgenus Strobus by the presence of smooth wall of ray tracheid and into the subgenus Eupitis by the presence of dentate wall of ray tracheid.

백합과 시금치의 葉肉, 花瓣 및 花粉으로부터 遊離 된 原形質體의 融合

蘇雄永 全北大學校 生物學硏究所 1980 生物學硏究年報 Vol.1 No.-

From the petal and leaf mesophyll cells of lily (Lilium regale) and the leaf mesophyll cells of sipnach (Spinacea oleracea), protoplasts were isolated in the mixed solution of 0.8M D-manitol, 1% macerozyme (or 0.1% pectolyase) and 2% cellulase. And then intra-and interspecific fusions of the protoplasts were attempted in the solution of 0.05M CaCl_2(pH 10.5) and different concentrations of D-manitol under different temperature and light conditions. Under the optimal condition for fusion, i.e. 0.5M D-manitol, 37℃ and 50 lux light, fusion of the protoplasts from spinach mesophyll and lily petal attained 7.1% and that from lily petal and leaf 4.6%, while fusion between the protoplasts from lily mesophyll reached up to 25%. Fusion between the protoplasts, which had been isolated from lily pollen grains by the treatment of a mixture of 15% sucrose, 1% macerozyme, 2% cellulase and 1% helicase, was also accomplished under the above optimal condition.

참당귀(Angelica fifas Nakai)의 캘러스로부터 체세포배 발생에 미치는 계대배양 주기의 영향

최용의,소웅영 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Embryogenic callus was produced form leaf segments cultured on medium containing 1.0 mg/L 2,4-D. The callus gave rise to somatic embryos after transfer the embryogenic callus to medium without 2,4-D. Also, somatic embryogenesis occured from aged callus on medium containing 2,4-D. To investigate the effects of culture periods on somatic embryogenesis the embryogenic calli were cultured for 2, 4, 6 and 8 weeks on medium containing 1.0 mg/l 2,4-D and then transferred to medium with or without 2,4-D. In prolonged preincubation, somatic embryos were formed early and at high frequency and their development normally occured. When subcultured on medium containing 2,4-D, the callus preincubated for 6 to 8 weeks was capable of producing somatic embryos. Histological observation revealed that 4 weeks-old embryogenic callus on medium containing 2,4-D was composed of mainly typical embryogenic cells, whereas the 8-weeks-old callus was composed of nonembryogeinc cells and that. In the developing state of somatic embryo on medium without 2,4-D, all the cells of callus were in vacuolated and elongated except developing somatic embryos.

담배(Nicotiana glauca) 카루스로 부터 Protoplast 및 Subprotoplast의 分離

呂邑東,蘇雄永 전북대학교 유전공학연구소 1988 遺傳工學硏究所報 Vol.1 No.1

As tools for the genetic modification of plants, the protoplasts were isolated from the green callus of Nicotiana glauca and were partitioned to subprotoplasts by gentle press operation. The isolation of protoplasts was taken for 5 hours in the enzyme solution(cellulase Onozuka R-10, 2%, macerozyme R-10, 1%, D-mannitol, 13% pH, 5.8)on the shaking bath(30℃, 70 strokes/min). The mean diameter was 45. 47±23.28μm and the mean volume was 4.92×10^-8 ㎤. The vacuoles was more easily observed by means of the deletion of cytoplasm and the destrution of cytoplasmic strands. After treated for 5 hours with the same enzyme solution for isolation of protoplasts, the callus clusters were pressed for 5 minutes by pincette through cover glass on them. The protoplasts were partitioned to about five subprotoplasts containing miniprotoplasts and cytoplasts. The ranges of diameter of miniprotoplasts and cytoplasts were 8.53-46.18μm and 8,58-52.18μm, respectively. One protoplast was partitioned to miniprotoplasts and cytoplasts at the ratio of 1:3 by the mean volume of protoplasts plsamolysed. But the observed ratio of miniprotoplast to cytoplast was 1:0.44. This result indicates that cytoplasts were more easily destructed than miniprotoplasts containing nucleus.

물오리나무의 둘레에 따른 維管束形成層과 材要素의 變化

韓京植,蘇雄永 又石大學校 1988 論文集 Vol.10 No.-

The changes in the vascular cambium and wood elements of Alnus hirsuta (Sprecht) Ruprecht have been investigated in relation to the girth of the tree axis. The fusiform initials gain a gradual size increase to start and later remain more or less constant in their length and width. The ray initials gradually increase in height and later remain more or less constant, while the width of the ray initials keep constant. Wood elements undergo considerable changes in their dimension with the growing girth of the the tree axis and their changing trends are equal to the vascular cambium.