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RISS 인기검색어
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- 저자 : Wenyi Tao (검색결과 3 건)
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Wenyi Tao,Jianghua Li,Long Liu,Jing Wu,Jun Sun,Guocheng Du,Jian Chen 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.4
Statistics-based experiment designs were used to optimize the culture medium (glucose, yeast extract, IPTG,tween-60, and CaCl2) for cutinase production by recombinant Escherichia coli. A 25-1 fractional factorial design augmented with center points revealed that glucose, yeast extract, and IPTG were the most significant factors, whereas the other factors were not important within the levels tested. The method of steepest ascent was used to approach the proximity of optimum, followed by a central composite design to develop a response surface for culture condition optimization. The optimum culture medium for cutinase production was found to be: glucose 33. 92 g/L, yeast extract 30.92 g/L,and IPTG 0.76 g/L. A cutinase production of 145.27±1.5 U/mL, which was in agreement with the prediction, was observed in triplicate verification experiments. The results obtained here verified the effectiveness of the applied methodology and may be helpful for cutinase production on an industrial scale.
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Bairui Tao,Rui Miao,Wenyi Wu,Fengjuan Miao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.12
In this paper, a three-dimensional graphene wrapped silicon nanowire (SiNWs) architecture was synthesized by electrochemical exfoliation method and proposed to be the counter electrode for dye-sensitized solar cells (DSSCs). The results show that the few high-quality layers of graphene sheets have been obtained by electrochemical exfoliation method. The size of graphene is distributed from 550 nm to 650 nm, appropriately dispersed onto the SiNW supporter. The graphene/SiNW nanostructure still vertically aligned to the substrate finely. The channel between each wire is clear. The fabricated graphene/SiNWs electrode is a promising structure for future applications. The performance of the graphene/SiNWs as the counter electrode is found to be dependent on its dispersion in the whole backbone, with better dispersion offering more surface areas for the catalytic reduction reaction. Electrochemical tests reveal that the DSSC with graphene/SiNWs exhibits higher electro-catalytic activity and lower charge transfer resistance, suggesting that the 3D structure presents a potential way to fabricate low-cost, integrated and metal-free counter electrode for high-performance DSSCs. The DSSC based on graphene/SiNWs has an open-circuit voltage (Voc) of 738.11 mV, short-circuit current density (Jsc) of 15.48mA cm 2, fill factor (FF) of 0.67 and conversion efficiency (η) of 7.66%.
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Han, Xiaoqing,Luan, Tao,Sun, Yingying,Yan, Wenyi,Wang, Dake,Zeng, Xianlu Korean Society for Molecular and Cellular Biology 2020 Molecules and cells Vol.43 No.9
Myeloid-derived suppressor cells (MDSCs) promote tumour progression by contributing to angiogenesis, immunosuppression, and immunotherapy resistance. Although recent studies have shown that microRNAs (miRNAs) can promote the expansion of MDSCs in the tumour environment, the mechanisms involved in this process are largely unknown. Here, we report that microRNA 449c (miR-449c) expression was upregulated in myeloid progenitor cells upon activation of C-X-C motif chemokine receptor 2 (CXCR2) under tumour conditions. MiR-449c upregulation increased the generation of monocytic MDSCs (mo-MDSCs). The increased expression of miR-449c could target STAT6 mRNA in myeloid progenitor cells to shift the differentiation balance of myeloid progenitor cells and lead to an enhancement of the mo-MDSCs population in the tumour environment. Thus, our results demonstrate that the miR-449c/STAT6 axis is involved in the expansion of mo-MDSCs from myeloid progenitor cells upon activation of CXCR2, and thus, inhibition of miR-449c/STAT6 signalling may help to attenuate tumour progression.
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