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Comparison of volatile components in fresh and dried Zanthoxylum bungeanum Maxim
Wenlin Zhang,Si Tan,Wanpeng Xi,Jianlei Yang,Qinhong Liao,Jianbin Lan,Yukui Lv,Jianmin Tang 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.4
Fresh and dried Zanthoxylum bungeanumMaxim volatiles of two main cultivars including Dahongpaoand Meihuajiao, were determined through GC–MS andcompared. In all the tested samples, linalool, D-limonene,eucalyptol, 3-nonanone, and b-myrcene were identified asthe five predominant components. The percentages of thesecomponents in fresh Dahongpao were 23.89%, 21.04%,7.46%, 5.63% and 5.87%, respectively. Similar percentages,27.28%, 17.62%, 6.39%, 1.66% and 7.8%, werefound in dried Dahongpao. In general, the contents oflinalool and b-myrcene in dried Dahongpao and Meihuajiaowere slightly higher than those in fresh samples,whereas the contents of D-limonene, eucalyptol, and3-nonanone were lower. Partial least squares discriminantanalysis results showed that the two cultivars could beclearly differentiated based on volatiles, whereas, the freshand dried Zanthoxylum bungeanum Maxim samples couldnot. This demonstrated that the drying process had nosignificant effect on the volatiles.
Thermal Model and Numerical Simulation of Selective Laser Alloying Process of Elemental Ti and B
Wenlin Ye,Yuan Tian,Dan Zhou,Peisheng Li,Jie Lei,Ying Zhang 대한금속·재료학회 2021 METALS AND MATERIALS International Vol.27 No.8
TiB2 has excellent performances in terms of wear resistance and corrosion resistance, mechanical strength, high elasticmodulus and high compressive strength, hardness, but its application is still limited due to its poor manufacturability. It isexpected to provide a transforming technology for its application by implementing additive manufacture of TiB2. The maincontent of this paper is to propose an innovative technology, selective laser alloying (SLA), using elemental powders ofTitanium and Boron provides a transforming technology for the application of TiB2by enabling its 3D forming from a puresimulation perspective. In this paper, a model of the in-situ reaction process is proposed. By considering the concentrationdistribution of Ti, B and TiB2,the effects of chemical reaction rate and laser power on the evolution of the free solid–liquidinterface and species transport are discussed. The laser energy consumed in the SLA process and the energy released bythe chemical reaction were theoretically derived. It showed that the heat released from the exothermic reaction betweenTitanium and Boron is an important energy source which can reduce the energy input and help improve the manufacturingefficiency of the SLA process; the chemical reaction rate has a significant influence on the concentration distribution of Ti,B and TiB2;the volume and existence time of molten pool and the heat source of reaction strongly relies on the chemicalreaction rate and the laser power.
Wenlin Ye,Jin Bao,Jie Lei,Yicheng Huang,Zhihao Li,Peisheng Li,Ying Zhang 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.1
A mesoscopic simulation based on random packing powder bed model was established to study the heat behavior of CP-Tiduring selective laser melting. The characteristics of the molten pool under the interaction of laser and powder, and theinfuence of laser power on the thermal behavior, hydrodynamics and surface morphology evolution of the molten pool werestudied. The results show that with the increase of laser power, the maximum temperature, temperature change rate, lifetimeof molten pool and size are greatly improved. In addition, the characteristics and heat behavior of the molten pool under thedouble track are mainly studied in this study. It is found that the maximum temperature, lifetime, and the length and width ofthe molten pool of the second track are higher than those in the frst, and with the increase of laser power, the length widthratio of the second track in molten pool becomes larger
Truncated Adenomatous Polyposis Coli Mutation Induces Asef-Activated Golgi Fragmentation
Kim, Sang Bum,Zhang, Lu,Yoon, Jimok,Lee, Jeon,Min, Jaewon,Li, Wenlin,Grishin, Nick V.,Moon, Young-Ah,Wright, Woodring E.,Shay, Jerry W. American Society for Microbiology 2018 Molecular and cellular biology Vol.38 No.17
<P>Adenomatous polyposis coli (APC) is a key molecule to maintain cellular homeostasis in colonic epithelium by regulating cell-cell adhesion, cell polarity, and cell migration through activating the APC-stimulated guanine nucleotide-exchange factor (Asef). The APC-activated Asef stimulates the small GTPase, which leads to decreased cell-cell adherence and cell polarity, and enhanced cell migration. In colorectal cancers, while truncated APC constitutively activates Asef and promotes cancer initiation and progression, regulation of Asef by full-length APC is still unclear. Here, we report the autoinhibition mechanism of full-length APC. We found that the armadillo repeats in full-length APC interact with the APC residues 1362 to 1540 (APC-2,3 repeats), and this interaction competes off and inhibits Asef. Deletion of APC-2,3 repeats permits Asef interactions leading to downstream signaling events, including the induction of Golgi fragmentation through the activation of the Asef-ROCK-MLC2. Truncated APC also disrupts protein trafficking and cholesterol homeostasis by inhibition of SREBP2 activity in a Golgi fragmentation-dependent manner. Our study thus uncovers the autoinhibition mechanism of full-length APC and a novel gain of function of truncated APC in regulating Golgi structure, as well as cholesterol homeostasis, which provides a potential target for pharmaceutical intervention against colon cancers.</P>
Zhao, Jiagang,Sun, Woong,Cho, Hyo Min,Ouyang, Hong,Li, Wenlin,Lin, Ying,Do, Jiun,Zhang, Liangfang,Ding, Sheng,Liu, Yizhi,Lu, Paul,Zhang, Kang American Society for Biochemistry and Molecular Bi 2013 The Journal of biological chemistry Vol.288 No.1
<P>Spinal cord injury (SCI) results in devastating motor and sensory deficits secondary to disrupted neuronal circuits and poor regenerative potential. Efforts to promote regeneration through cell extrinsic and intrinsic manipulations have met with limited success. Stem cells represent an as yet unrealized therapy in SCI. Recently, we identified novel culture methods to induce and maintain primitive neural stem cells (pNSCs) from human embryonic stem cells. We tested whether transplanted human pNSCs can integrate into the CNS of the developing chick neural tube and injured adult rat spinal cord. Following injection of pNSCs into the developing chick CNS, pNSCs integrated into the dorsal aspects of the neural tube, forming cell clusters that spontaneously differentiated into neurons. Furthermore, following transplantation of pNSCs into the lesioned rat spinal cord, grafted pNSCs survived, differentiated into neurons, and extended long distance axons through the scar tissue at the graft-host interface and into the host spinal cord to form terminal-like structures near host spinal neurons. Together, these findings suggest that pNSCs derived from human embryonic stem cells differentiate into neuronal cell types with the potential to extend axons that associate with circuits of the CNS and, more importantly, provide new insights into CNS integration and axonal regeneration, offering hope for repair in SCI.</P>
Li Yuchan,Zhao Jun,Chen Hua,Yu Xia,Li Hui,Zhang Yu,Feng Liang,Wu Zhe,Xie Wenlin,Hou Dabin,Yu Ma 한국식물생명공학회 2023 Plant biotechnology reports Vol.17 No.2
Bupleurum chinense DC is an important medicinal plant with many active ingredients that are used for the treatment of different types of diseases and valued in pharmaceutical markets. In vitro shoot regeneration can efficiently contribute to the improvement of B. chinense. In the present study, we investigated the effects of the explant type and plant growth regulators (PGRs) on embryogenic callus induction and plant regeneration in B. chinense. Our investigation demonstrated that 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) combined with 1 mg/L thidiazuron (TDZ) played a major role in promoting callus induction from leaf, hypocotyl and stem 2 explants, whereas the most effective treatment for stem 1 callus formation was Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-D, 0.5 mg/L 6-benzyladenine (BA) and 0.5 mg/L kinetin (Kin). The highest shoot regeneration rate (57.14%) was obtained from hypocotyl-induced calli in MS medium with 0.5 mg/L Kin after 12 weeks of cultivation. This regeneration protocol can be used in large-scale cultivation and may be useful for future genetic modifications of B. chinense.
Yin Ronghuan,Yin Ronglan,Bai Man,Fan Yixing,Wang Zeying,Zhu Yubo,Zhang Qi,Hui Taiyu,Shen Jincheng,Feng Siyu,Bai Wenlin 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.4
Objective: The objective of this study was to investigate the effects of N6-Methyladenosine modification-circRNA-zinc finger protein 638 (m6A-circRNA-ZNF638) on the induced activation of secondary hair follicle (SHF) stem cells with its potential mechanisms in cashmere goats. Methods: The m6A modification of ZNF638 was analyzed using methylation immunoprecipitation with real-time quantitative polymerase chain reaction technique in SHF stem cells. The effects of circRNA-ZNF638 on the induced activation of SHF stem cells in m6A dependence were evaluated through the overexpression of circRNA-ZNF638/its m6Adeficient mutants in circRNA-ZNF638 knockdown SHF stem cells. The competitive binding of miR-361-5p to circRNA-ZNF638/Wnt5a 3′- untranslated region was analyzed through Dual-luciferase reporter assay. Results: The m6A-circRNA-ZNF638 had significantly higher transcription at anagen SHF bulge of cashmere goats compared with that at telogen, as well as it positively regulated the induced activation of SHF-stem cells in cashmere goats. Mechanismly, m6A-circRNA-ZNF638 sponged miR-361-5p to heighten the transcriptional expression of Wnt5a gene in SHFstem cells. We further demonstrated that the internal m6A modification within circRNAZNF638 is required for mediating the miR-361-5p/Wnt5a pathway to regulate the induced activation of SHF stem cells through an introducing of m6A-deficient mutant of circRNAZNF638. Conclusion: The circRNA-ZNF638 contributes the proper induced activation of SHF-stem cells in cashmere goats in m6A-dependent manner through miR-361-5p/Wnt5a axis.