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Cui Yongzhong,Wang Weiwei,Lu Qin,Wu Hai‐Xia,Ling Xiaofei,Liu Juan,Zhang Jinwen,Chen Hang 한국곤충학회 2022 Entomological Research Vol.52 No.3
Energy supply is a major concern of human survival and development, and that concern demands new sources of renewable clean energy. Cellulose, as a renewable energy material is affected by low activity and high cost of cellulose. Studies of cellulose degrading bacterial microbiota in insect intestine have revealed that these potential microbes can be used as a rich resource of cellulose degrading strains. Here, the present study involves metagenomic sequencing of intestinal microbes of Macrotermes annandalei by Illumina PE150 and a total of 401,190 scaftigs were obtained. Open Reading frame (ORF) species annotation showed that the largest proportion of them belong to the phylum of Bacteroides, followed by Protobacteia, Firmicutes and Mucoromycota. Mapping to the CAZy database indicates that 4,539 genes encoding of glycoside hydrolase belongs to 109 distinct CAZy families. The results provide a theoretical basis for exploring new candidate bacteria for efficient cellulose degradation to obtain high cellulose resource yield.
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Shuang Sun,Weiwei Cui,Ying Dong,Qing Wang 한국고분자학회 2019 Macromolecular Research Vol.27 No.5
An organoid is a kind of three-dimensional cell culture system that is highly similar to the source tissues or organs in the body. Recently, organoid therapy has become a hot topic in the field of fundamental researches and clinical treatments. Type 1 diabetes mellitus (T1DM) is caused by the absolute lack of insulin. Exogenous insulin supplement, the only treatment currently available for T1DM, has nonideal effect due to the poor compliance. An implantable and sustainable strategy of T1DM is needed and the organoids theoretically provide the basis for treatment. However, the current structure and function of organoids are different from that of real organs or tissues. The organic scaffolds can effectively support the growth of organoids, which can increase the similarity of organoids to the real structure. In order to find suitable scaffold materials for islet β-like cells (IBCs) organoids, poly (lactic-co-glycolic acid) (PLGA) polymer films were prepared to act as induced carriers for IBCs derived from bone marrow mesenchymal stem cells (BMSCs). The surface of PLGA film was simply modified through dopamine (DA) coating (i.e., DA-PLGA) and then liraglutide (LIR) was fixed on the surface of PLGA film via the DA coating (i.e., LIR-DA-PLGA). The results proved that DA could enhance the hydrophilicity and cell proliferation and LIR-DA-PLGA was suitable to act as inducing carriers. In subsequent experiments, LIR-DA-PLGA scaffolds would be prepared to culture organoids and transplanted into the body of diabetic rat model to regulate blood glucose lever.
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Yuan Song,Xiangqun Wu,Di Yang,Fang Fang,Lingshi Meng,Ya Liu,Weiwei Cui 한국식품영양과학회 2020 Journal of medicinal food Vol.23 No.4
Much research has indicated that alcoholic liver disease (ALD) is associated with oxidative stress and inflammation induced by ethanol, and that numerous antioxidants could effectively alleviate such injuries. Moreover, recent studies have identified andrographolide (AD) as having strong anti-inflammatory and antioxidant activities, which can block oxidative damage associated with nuclear factor kappa B (NF-κB)-mediated inflammation. However, the biological role and potential mechanism of AD in its protection against ALD have not been fully characterized. To observe the possible effect of AD, male C57BL/6J mice received ethanol through intragastrical gavage for 12 weeks in this study. The ethanol group was separated into five subgroups: (1) model group (n = 10); (2) silymarin group (0.1 mg/g body weight [BW], n = 10); (3) AD (0.05 mg/g BW) group (n = 10); (4) AD (0.1 mg/g BW) group (n = 10); and (5) AD (0.2 mg/g BW) group (n = 10). Mice in AD groups were treated orally by gavage once per day. The experimental results show that serum aminotransferase, liver lipids, lipid peroxidation, and antioxidant capacities were significantly changed in the model group after alcohol treatment, and the liver tissue histological findings showed pathological changes. Compared with the model group, treatment with AD improved serum aminotransferase, liver function, lipid accumulation, and hepatic reactive oxygen species levels. And AD decreased the hepatic NF-κB and tumor necrosis factor alpha (TNF-α) protein expression of ALD mice. This research demonstrated that AD can alleviate liver pathological injury and oxidative stress in mice exposed to ethanol by decreasing the expression of NF-κB and TNF-α.
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( Zhiping Peng ),( Bo Xu ),( Antonio Marcel Gates ),( Delong Cui ),( Weiwei Lin ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.7
In the dynamic cloud computing environment, to ensure, under the terms of service-level agreements, the maximum efficiency of resource utilization, it is necessary to investigate the online dynamic management of virtual machine resources and their operational application systems/components. In this study, the feasibility and properties of the division of virtual machine resources on the cloud platform, using the virtual machine cluster as the management unit, are investigated. First, the definitions of virtual machine clusters are compared, and our own definitions are presented. Then, the feasibility of division using the virtual machine cluster as the management unit is described, and the isomorphism and reconfigurability of the clusters are proven. Lastly, from the perspectives of clustering and cluster segmentation, the dynamics of virtual machines are described and experimentally compared. This study aims to provide novel methods and approaches to the optimization management of virtual machine resources and the optimization configuration of the parameters of virtual machine resources and their application systems/components in large-scale cloud computing environments.
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