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Kim, K.-H.,Wasano, N.,Ohba, M. 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1
Four Lepidoptera-specific reference strains of Bacillus thuringiensis, belonging to serovars sumiyoshiensis(H3a:3d), fukuokaensis(H3a:3d:3e), darmstadiensis(H10a:10b) and japonensis(H23), which produce spherical parasporal inclusions, were examined for comparative characterization of δ-endotoxins. SDS-PAGE profiles of the alkali-solubilized parasporal inclusions revealed the presence of single major protein bands of 130 kDa in the four strains. Chymotrypsin and trypsin treatment of the proteins gave profiles different from those of the strains HD-1(serovar kurstaki, H3a:3b:3c) and T84Al(serovar sotto, H4a:4b). Also, minor variations were observed in proteolysis profiles among the four strains. The LC_50 values of purified parasporal inclusions for the silkworm(Bombyx mori) larvae were 7·35, 6·45, 3·08 and 2·63 ㎍ g^-1 diet, respectively, showing that their toxicity levels were 5-15 times lower than that of the strain HD-1 (0·49㎍ g^-1 diet). Analysis by immunodiffusion and immunoblotting with polyclonal antisera revealed that parasporal inclusion proteins of the four strains are highly related, whereas they shared few or no common anitgens with those of the strains HD-1, T84Al and Buibui(serovar japonensis).