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- 저자 : Wanfeng Liu (검색결과 2 건)
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Graphene Decorated with Hierarchical CuS Nanoplates: Enhanced Photocatalytic Performance
BIN ZENG,Wanfeng Liu,WUJUN ZENG,Can Jin 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2018 NANO Vol.13 No.3
Graphene decorated with hierarchical CuS nanoplates (CuS NP-G) was synthesized using a microwave method to be used as a photocatalytic material. The incorporation of graphene into hierarchical CuS nanoplates was confirmed by structural, morphological and optical characterizations. The photocatalytic performance of the nanocomposite was evaluated. This study confirmed that the introduction of graphene was an effective way not only to improve the structural stability and service durability of the composite, but also to improve its solar photocatalytic activity by promoting the electron transfer and charge separation of hierarchical CuS nanoplates.
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Novel splice isoforms of pig myoneurin and their diverse mRNA expression patterns
Guo, Xiaohong,Li, Meng,Gao, Pengfei,Cao, Guoqing,Cheng, Zhimin,Zhang, Wanfeng,Liu, Jianfeng,Liu, Xiaojun,Li, Bugao Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.10
Objective: The aim of this study was to clone alternative splicing isoforms of pig myoneurin (MYNN), predict the structure and function of coding protein, and study temporal and spatial expression characteristics of each transcript. Methods: Alternative splice isoforms of MYNN were identified using RNA sequencing (RNA-seq) and cloning techniques. Quantitative real-time polymerase chain reaction (qPCR) was employed to detect expression patterns in 11 tissues of Large White (LW) and Mashen (MS) pigs, and to study developmental expression patterns in cerebellum (CE), stomach (ST), and longissimus dorsi (LD). Results: The results showed that MYNN had two alternatively spliced isoforms, MYNN-1 (GenBank accession number: KY470829) and MYNN-2 (GenBank accession number: KY670835). MYNN-1 coding sequence (CDS) is composed of 1,830 bp encoding 609 AA, whereas MYNN-2 CDS is composed of 1,746 bp encoding 581 AA. MYNN-2 was 84 bp less than MYNN-1 and lacked the sixth exon. MYNN-2 was found to have one $C_2H_2$ type zinc finger protein domain less than MYNN-1. Two variants were ubiquitously expressed in all pig tissues, and there were significant differences in expression of different tissues (p<0.05; p<0.01). The expression of MYNN-1 was significantly higher than that of MYNN-2 in almost tissues (p<0.05; p<0.01), which testified that MYNN-1 is the main variant. The expression of two isoforms decreased gradually with increase of age in ST and CE of MS pig, whereas increased gradually in LW pig. In LD, the expression of two isoforms increased first and then decreased with increase of age in MS pig, and decreased gradually in LW pig. Conclusion: Two transcripts of pig MYNN were successfully cloned and MYNN-1 was main variant. MYNN was highly expressed in ST, CE, and LD, and their expression was regular. We speculated that MYNN plays important roles in digestion/absorption and skeletal muscle growth, whereas the specific mechanisms require further elucidation.
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