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Streptococcus mutans의 우식 활성에 미치는 Propolis의 효과
송완엽,백병주,주훈,김대범,송요한 大韓小兒齒科學會 1995 大韓小兒齒科學會誌 Vol.22 No.1
Propolis, a natural resinous product collected by honey bees, is known to exhibit various biological activities including anti-microbial, anti-inflammatory, and anti-tumor activities. The present study was designed to investigate the effects of ethanol extracted propolis(EEP) on the growth, glucosyltransferase(GTase) activity, and adherence to the saliva-coated hydroxyapatite(S-HA) beads of Streptococcus mutans JC-2(serotype c), S.mutans LMM-7(serotype e), and S. mutans OMZ-175(serotype f) in vitro. We found that the EEP had minimal inhibitory concentrations of 500-1000㎍/㎖ against S.mutans strains when these strains were cultured for 48 hours, and inhibited both water-insoluble and water-soluble glucan synthesizing GTase activities of S. mutans strains to the 93-100% at the concentration of 1600㎍/㎖, and also we found that the EEP inhibited the adherence of S. mutans strains to S-HA beads to the 76-96% at the concentrations of 200㎍/㎖. These results indicate that EEP has anti-cariogenic activity in vitro.
A New Selective Medium for Detecting Acidovorax avenae subsp. avenae in Rice Seeds
Song, Wan-Yeob,Kang, Mi-Hyung,Kim, Hyung-Moo The Korean Society of Plant Pathology 2000 Plant Pathology Journal Vol.16 No.4
A selective agar medium was developed and tested for the isolation of Acidovorax avenae subsp. avenae, the causal bacterial pathogen of bacterial brown stripe, from rice seeds. The new selective agar medium, designated sorbitol pyroglutamic acid agar (SPA) medium, contained 0.5 g of $K_2$HPO$_4$, 3.0 g of Na$_2$HPO$_4$, 2.0 g of D-sorbitol, 0.2 g of L-pyroglutamic acid, 10.0 $m\ell$ of tween 80, 40.0 mg of victoria blue B, 15.0 g of agar, 150.0 mg of ampicillin and 25.0 mg of vancomycin per litter. Colonies of A. avenae subsp. avenae on SPA medium were smooth, round, convex, shiny, blue and 1.5-2.0 mm in diameter 4 days after incubation at 28$^{\circ}C$. Blue colored colony having dark blue zone was typical type of A. avenae subsp. avenae colonies on the medium. Mean recovery of 8 isolates of A. avenae subsp. avenae on the selective SPA medium was 95.8% in comparison to that on KB medium. The saprophytic bacteria were reduced to 97.9% on SPA medium compared to those on KB medium. Most of other rice seedborne bacteria as well as reported pathogenic bacteria were failed to grow on SPA medium. This medium was highly selective for recovering A. avenae subsp. avenae from rice seed samples, and it could be used to enhance the recovery of this bacterium from rice seed samples, which may be contaminated with large numbers of competing microorganisms.
宋完燁,金炯武,盧台煥,李斗求 전북대학교 농업과학기술연구소 1996 農大論文集 Vol.27 No.1
Twenty phenolic compounds originated from plants were tested in nutrient-broth bioassay for screening inhibitory activity to rice seedborne Pseudomonas avenae, P.glumae and Xanthomonas oryzae pv. oryzae. Flavone and esculetin strongly inhibited X. o. pv. oryzae only. Myricetin inhibited .P. glumae and X. o. pv. oryzae but showed strong minimal inhibition concentration and minimal bactericidal concentration under a concentration of 25μg/ml. Cinnamic acid ethylester, naringenin and phloretin showed significant inhibitory activity towards tested all strains of P. avenae, P. glumae and X. o. pv. oryzae. Cinnamic acid ethylester was moderately inhibitory (100-200 μg/ml) and naringenin (50- 200 μg/ml) and phloretin (25-50 μg/ml) were strongly inhibitory. At a concentration of 100 μg/ml, phloretin were found to be bactericidal to all the tested strains of three species.
중앙난방 시스템의 제어방법에 따른 난방성능 및 에너지소모량 특성 연구
송재엽(Jae-Yeob Song),양완연(Wan-Youn Yang),안병천(Byung-Cheon Ahn) 한국지열·수열에너지학회 2011 한국지열에너지학회논문집 Vol.7 No.1
In this study, heating performance and energy consumption characteristics with control strategies for central heating system were researched by the simulation. The simulation analysis is made by TRNSYS ver. 15 with the actual data. The parametric study on proportional factor, control time interval and outdoor air temperatures changes were done to compare control characteristics and energy performance, respectively. As a result, the simulation results with various parameter changes show good heating performance and energy saving.
Fusarium oxysporum f.sp.lagenariae에 의한 박 시들음병(萎凋病)
姜美亨,宋完燁,金炯武,李光植 전북대학교 농업과학기술연구소 1995 農大論文集 Vol.26 No.1
Acute wilting of bottle gourd have occurred recently in watermelon growing areas in Korea. 963 fungal isolates were collected from the bottle gourd seeds, infected watermelon seedlings and grafted bottle gourd stocks. Among them 279 isolates (28%) were identified as Fusarium oxysporum. 9 isolates among 27 ones from bottle gourd seeds, 87 isolates among 246 ones from infected bottle gourd seedlings, 42 isolates among 258 ones from infected watermelon seedlings and 141 isolates among 432 ones from infected grafted rootstocks were identified to be F.oxysporum. F. oxysporum isolated from wilted bottle gourd was identified to be Fusarium oxysporum. f. sp. lagenariae on the basis of morphological characteristics of spores and conidio-phores and of pathogenicity. Infected bottle gourd plants by root-dipping inoculation appeared the acute wilting after 5 days and browning, wilting and then collapsing after 14 days. This pathogen also infected bottle gourd, Sintosa, Donga and Andong cucumber except for watemelon and Haneultari.
고추냉이 세균성 근경 부패병의 발생 및 병원균의 분리 동정
김형무,송완엽,강미형,소인영 전북대학교 농업과학기술연구소 1996 農大論文集 Vol.27 No.1
A disease occurrence of rhizome rot at the Japanese horse radish growing fields in Chonbuk area was investigated and soft rot-causing bacteria were isolated and identified by morphological and biochemical characteristics to verify a causal bacterium of rhizome soft-rot disease of Japanese horse radish. The rhizome soft-rot was occurred on April and continued to October·. It was most severe on August but decreased on September. Disease occurrence reflected regional difference such as 38% in Chonju and Iksan and 27% in Muju. Four major bacterial genera, Erwinia, Pseudomonas, Xanthomonas and Corynebacterium, were isolated and showed the isolation frequency of 54.6%, 17.2%, 21.2% and 6.8%, respectively. They also showed a seasonal fluctuation of isolation frequencies in genera and Erwinia sp. was the most dominant species during the high temperature season. Rhizome soft rot-causing isolates were selected by the pathogenicity test. They showed negative results in Gram's staining, growth in 5% NaCl and growth on KCN media but positive in casein hydrolysis and gelatin liquefaction. They utilized galactose, lactose, trehalose and citrate but did not utilize maltose, melibiose, raffinose and inullin. They also showed the soft-rot symptom on the petiole and rhizome of the horse radish but weak soft rot on potato tuber. This causal pathogen was identified as Erwinia carotovora subsp. wasabiae by these results of bacteriological characteristics.