서울시 대기오염과 호흡기 질환 환자와의 관련성에 대한 시계열적 연구

최병철,구정완,박성균,임현우,이강숙,이원철,박정일 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 서울시의 대기오염 수준이 호흡기 질환 외래 환자수에 미치는 단기 영향을 파악하고자 하였다. 방법 : 1997년 7월부터 1998년 6월까지 서울시의 대기오염자료와 호흡기 질환에 대한 의료보험자료를 분석하였다. 추세변동과 기상요인, 요일 및 주별 효과등의 교란요인을 제어하고 관련성을 평가하기 위하여 generalized additive model(GAM)을 이용하였다. 결과 : 교란요인을 제어한 기본모델에 각 대기오염 물질을 포함시켜 분석한 결과, CO는 모든 연령층에서 일정하게 당일 농도가 가장 유의한 영향을 미치는 것으로 나타났고(RR=1.041∼1.051), NO₂역시 3일전의 농도가 모든 연령층에서 가장 유의한 영향을 미쳤다(RR=1.024∼1.050). O₃와 SO₂는 4일전 농도부터 당일 농도까지 지연시간이 길게 나타나는 특징을 보였으며, 15세 이하에서는 3일전 농도가(O₃RR=1.047, 95 % Cl=1.045∼1.049, SO₂RR=1.018, 95 % Cl=1.017∼1.020), 15-64fll (O₃RR=1.035, 95 % Cl=1.032∼1.037, SO₂RR=1.037, 95 % Cl=1.035∼1.039)와 65세 이상 (O₃RR=1.042, 95 % Cl=1.033∼1.051, SO₂RR=1,029, 95 % Cl=1.022∼1.036)에서는 당일 농도가 가장 유의한 결과를 나타냈다. PM□은 유의성이 낮게 나타났는데 15세 이하는 하루 전 농도(RR=1.011, 95 % 71=1.010∼1.013), 15∼64771(RR=1.021, 95 % Cl=1.019∼1.023)와 65세 이상(RR=1.025, 95 % Cl=1.018∼1.032)은 당일 농도가 유의하게 나타났다. 두 포염물질씩을 기본모델에 포함시켜 분석한 결과 CO, NO₂, O₃는 상대위험비가 '단일 오염물질모델'의 값과 거의 일정하게 통계적 유의성을 유지하였으나 SO₂는 15세 이하에서 NO₂와 같이 포함된 모델에서 통계적 유의성을 상실하였다. PM□은 모든 연령층에서 CO와 같이 포함된 모델에서 통계적 유의성을 상실하여 '단일 오염물질모델'에서 나타난 유의성이 CO에 의한 교란작용의 결과임을 알 수 있었다. 또한 15세 이하에서 NO₂, 15∼64세와 65세 이상에서 502와 같이 포함된 모델에서 역시 유의성을 상실하여 본 연구에서 살펴본 호흡기 질환에 미치는 PM□의 영향은 크지 않은 것으로 나타났다. 결론 : 본 연구의 결과는 서울시의 일상적인 대기오염이 여러 호흡기질환의 발생을 증가시킬 수 있음을 보여주었다. 따라서 대기오염의 효율적 규제에 대해 보다 적극적인 대책이 마련되어야 할 것이다. Objectives : This study was conducted to investigate whether air pollution levels in Seoul have short term effects on respiratory disease outpatients. Methods : We analyzed five air pollutants (CO, NO₂, O₃, SO₂, PM□) and daily counts of outpatient for respiratory disease divided by age group ((15yrs, 15-64yrs, 654yrs)during the period of July 1997-June 1998 using Generalized Additive Model (GAM) . Results : After controlling for seasonal trends, day of the week, week of the month, temperature and humidity, CO (RR=1.041 ∼1.051, 0-day lag) and NO₂(RR=1.024∼1.050, 3-day lag) were significantly associated with daily counts for respiratory disease in all age groups. O₃and SO₂were associated, with a 3-day lag in the age group under 15 years (O₃RR=1.047, 95% Cl=1.045-1.049; SO₂RR=1.018, 95% Cl=1.017-1.020) and with a 0-day lag in the age group 15 to 64 years (03 RR=1.035, 95% Cl=1.032-1.037; 502 RR=1.037, 95% Cl=1.035-1.039), in the age group over 65 years (O₃RR=1.042, 95% Cl=1.033-1.051; SO₂RR=1.029, 95% Cl=1.022-1.036). PMlo was weakly associated, with a 1∼day lag In the age group under 15 years (RR=1.011, 95% Cl=1.010-1.013) and with a 0-day lag in the age group 15 to 64 years (RR=1.021, 95% Cl=1.019-1.023), in the age group over 65 years(RR=1.025, 95% Cl=1.018-1.032) . In the' two pollutant model', the magnitudes of the recta alive risk for CO, NO₂nd O₃were maintained, but SO₂was not assoclated with daily counts for respiratory disease in the age group under 15 years after controlling for NO₂-PM□ was not associated in all age groups after controlling for CO, so the association with PM□ in the' single pollutant model' might be confounded by CO. In this study, the impact of PM□ on the respiratory disease was not large as compared with other pollutants. Conclusions : Air pollution in Seoul may Increase the Incidence of the respiratory disease, so more positive attention for the control of air pollution should be paid.

스포츠에 의한 성장기 요추추체종판 장해 분석

김민섭,이종영,이충영,이승훈,임완기,강은균,이종호 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

Apophyseopathy in human growing-up period is divided in three parts, early stage, advancing stage, and ending stage which are analyzed by X-ray test. The tuber of kantenabtrennung, schmorl and rear oxhorn dissociation are one of the these barrier process. We assume that the fracture of apophysis by overusing is the main cause of these barriers.

The Study of the origins of Curcuma Spp .

WHANG wan kyun,SHIN sang soo,KIM do hun,LEE sung wan,KIM sung kun,CHUNG sung hee 大韓藥學會 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.1

Expression and secretion of Clostridium thermocellum endoglucanase A gene (celA) in different Saccharomyces cerevisiae strains

Chung, Dae-Kyun,Shin, Dong-Ha,Kim, Byung-Woo,Nam, Jae-Kook,Han, In-Seob,Nam, Soo-Wan 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

To develop effective and powerful probiotics, Saccharomyces cerevisiae strains producing cellulolytic enzymes were genetically engineered. Two plasmids were constructed in which the carboxymethyl cellulase (CMCase) gene of Clostridium thermocellum was connected in frame with GAPDH or ADH1 promoter. The resultant plasmids were transformed into various S. cerevisiae host cells, and the expression level and secretion efficiency of the CMCase were examined. The difference in genetic background of host strains did affect significantly the cell growth and expression and secretion levels of CMCase. Irrespective of the nature of the host cells, the ADH1 promoter-employing plasmid showed a greater expression level and plasmid stability than the GAPDH promoter-based plasmid. By the optimal host-vector system, S. cerevisiae SEY2102 harboring pVT-CT1 plasmid (ADH1 promoter), the highest expression level of 277 unit CMCase/L was obtained.

Saccharomyces cerevisiae에서 Trichoderma Endoglucanase의 발현과 분비

남수완,김병우,신동하,김재범,신지원,정대균,정춘수 동의대학교 기초과학연구소 1999 基礎科學硏究論文集 Vol.9 No.1

The endoglucanase gene, egl6, of Trichoderma sp. was connected with the yeast ADH1 promoter, and the resultant plasmid, pVT-C4, was introduced into three S. cerevisiae host strains (YNN27, 2805, and SEY2102). Among each 80 transformants, the cell growth and expression level of endoglucanase were compared in test-tube cultivation, and three respective transformants for each host cells showing the highest expression level and cell growth were selected. When three recombinant yeast cells were batchwise cultivated for 48 hr in flask, the total activities of endoglucanase expressed were about 1140 unit/1 with 2805/pVT-C4, 1020 unit/l with SEY2102/pVT-C4, and 590 unit/l with (YNN27/pVT-C4. Irrespective of host strain, about 80% of the expressed endoglucanase was detected in the extracellular medium. In addition, it was also found that the recombinant enzyme was secreted into the culture medium as two major forms of lightly and heavily glycosylated proteins.

Saccharomyces cerevisiae에서 Clostridium thermocellum Endoglucanase 유전자의 구성적 발현

남수완,정대균,정봉현 동의대학교 기초과학연구소 1998 基礎科學硏究論文集 Vol.8 No.1

To develop an effective and powerful yeast probiotics, Saccharomyces cerevisiae strains producing cellulolytic enzymes were genetically engineered. We constructed two plasmids in which the endoglucanase A gene, celA, of Clostridium thermocellum was connected in frame with ADH1 or GAPDH promoter. These plasmids were transformed into various S. cerevisiae host strains, and then the cell growth, expression level and plasmid stability between the transformed yeast cells were examined in the flask culture. The difference in genetic background of host strains did affect significantly the cell growth and expression level of endoglucanase. Based on the higher levels of cell growth(5.4∼5.9 g-DCW/L) and plasmid stability(79∼85%), three host cells(YNN27, 2805 and SEY2102) and ADH1 promoter were selected as optimal host-vector systems for the constitutive expression of celA. The recombinant yeast produced about 200 unit/L of endoglucanase as a growth-associated manner in the batch fermentation, due to the increased cell growth of 11∼13 g-DCW/L. In addition, 25∼38% and 53∼64% of the endoglucanase activity were detected in the culture medium and periplasmic space, respectively. These results indicate that the signal sequence of celA functioned well in S. cerevisiae cells and the recombinant yeast cells can be employed for the production of probiotics.

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

일부 부인과 양성 질환자의 지방조직 및 혈청의 PCBs와 DDE 농도

박성균,이강숙,노영만,구정완,민선영,한진구,고원경,김미란,정재근,이종승,김진홍 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.1

목적 : 본 연구는 지방조직과 혈청의 PCBs와 DDE 농도를 측정하고, 이에 영향을 미치는 것으로 알려진 요인들과의 관련성을 평가하고자 하였다. 방법 : 유방암, 자궁내막증 등 유기염소계 화합물의 영향이 있는 것으로 알려진 호르몬 관련 질병을 제외한 부인과 환자 52명을 대상으로 지방조직과 혈청을 분석하였으며, 설문조사를 통하여 연령, 교육수준, 직업, 비만도, 흡연과 음주, 출산력, 수유력등을 조사하였다. 지방조직과 혈청의 PCBs와 DDE 농도는 가스크로마토그래피를 사용하여 측정하였다. 결과 : 지방조직의 경우, 13개 PCBs congeners 농도의 합은 중앙값이 48.29 ng/g, 기하평균값이 56.78 ng/g이었고, DDE 농도는 중앙값이 142.89ng/g, 기하평균값이 117.06 ng/g이었으며, 혈청의 경우는 PCBs congeners 농도의 합은 중앙값이 4.67 ㎍/L, 기하평균값이 4.85 ㎍/L이었고, DDE 농도는 중앙값이 1.75 ㎍/L, 기하평균값이 2.09 ㎍/L로 나타났다. 지방조직과 혈청의 DDE 농도 사이는 높은 상관성을 보였으나(r²=0.310, p=0,0002), PCBs는 상관성이 약한 것으로 나타났다(r²=0.029, p=0.2582). 영향요인을 범주화한 후 로그변환 시킨 지방조직과 혈청의 PCBs, DDE 평균의 차이를 분석한 결과, 지방조직의 DDE만이 출산자녀수(p=0.015), 초산연령 (p=0.014), 비만지수(p=0.035)와 연관성이 발견되었다 다중회귀분석의 결과 지방조직의 DDE는 출산자녀수가 적을수록, 초산연령이 늦을수록 증가하는 경향을 보였다. 결론 : 출산자녀수와 초산연령, 비만도가 지방조직의 DDE와 관련성을 보였으며, 또한 유기염소계 화합물의 장기간의 노출에 의한 영향을 평가하는데 있어서 혈청보다 지방조직이 더 유용한 생물지표임을 알 수 있었다. Objectives : This study was purposed to determine the levels of PCBs and DDE in adipose tissue and serum and to evaluate the relations with factors affecting these levels. Methods : We analyzed adipose tissues and sera from 52 gynecologic benign disease patients aged 27-78 years, except hormonal diseases such as breast cancer and endometriosis. We also surveyed age, education, occupation, body mass index (BMI), smoking, drinking, number of children, and duration of breastfeeding by questionnaires. Tissue and serum levels of PCBs and DDE were measured using gas chromatography. Results : The median and geometric mean levels of PCBs were 48.29 ng/g, 56.78 ng/g for adipose tissue and 4.67 ㎍/L, 4.85 ㎍/L for serum, and those of DDE were 142.89 ng/g, 117.06 ng/g for adipose tissue and 1.75 ㎍/L, 2.09 ㎍/L for serum, respectively. Adipose tissue and serum levels for DDE showed high correlation (r²=0.310, p=0.0002), but those of PCBs didn't (r²=0.029, p=0.2582). In analyses of the differences of the means of log transformed adipose tissue and serum PCBs and DDE levels for groups of potential covariates, only adipose tissue DDE levels were significantly associated with number of children (p=0.015), age at first birth (p=0.074) and BMI (p=0.035). In multiple regression analysis, adipose tissue DDE levels were significantly increased with decreasing number of children and increasing age at first birth. Conclusions : This study suggested that parity and adiposity were associated with levels of DDE in adipose tissue, and adipose tissue is a better biomarker than serum for evaluating the long-term exposure of organochlorines.

수삼의 지역별 연근별 인삼사포닌 함량 비교

이충렬(Chung Ryul Lee),황완균(Wan Kyun Whang),신차균(Cha Gyun Shin),이학성(Hak Sung Lee),한성태(Sung Tai Han),임병욱(Byung Ok Im),고성권(Sung Kwon Ko) 한국식품과학회 2004 한국식품과학회지 Vol.36 No.5

Flavonoids from Codonopsis lanceolata Leaves

Whang, Wan-Kyunn,Park, Kyun-Young,Chung, Sung-Hoon,Oh, In-Se,Kim, Il-Hyuk The Korean Society of Pharmacognosy 1994 생약학회지 Vol.25 No.3

For the investigation of resources in Codonopsis species, the studies were carried out to evaluate the pharmaco-constituents from the leaves of Codonopsis lanceolata (Campanulaceae) whose roots have been used to antitussive, expectorant, detoxicate, tonic, edible, etc. as a folk medicine in Korea. From the EtOAc and BuOH fractions of MeOH extract, three flavonoid compounds, $luteolin-7-O-{\beta}-_D-glucopyranoside$$(C_{21}H_{20}O_{1i},\;mp\;254{\sim}255^{\circ}$, compound 1), $luteolin-5-O-{\beta}-_D-glucopyranoside$$(C_{21}H_{20}O_{1i},\;mp\;279{\sim}281^{\circ}$, compound 2) and luteolin $(C_{15}H_{10}O_6,\;mp\;327{\sim}330^{\circ}$, compound 3) were isolated and identified on the basis of their physicochemical properties and spectroscopic evidences(UV, IR, $^1H-NMR$, $^{13}C-NMR$, MS etc.) in comparison with authentics respectively.