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Contributions of Parental Lineage on the Community Development of Rice Seed Bacterial Endophytes
( Denver Walitang ),김기윤 ( Kiyoon Kim ),( Aritra Roy Choudhury ),강연경 ( Yeongyeong Kang ),사동민 ( Tongmin Sa ) 한국환경농학회 2017 한국환경농학회 학술대회집 Vol.2017 No.-
There is relatively diverse groups of bacterial endophytes inhabiting the seeds of rice that could become the dominant sources of endophytes of rice host as bacterial endophytes are transmitted and conserved in the next generations. The objective of this study is to gain insights into the contributions of parental lineage and crossbreeding on the seed bacterial endophytic communities of two pure inbred lines exploring influence of the two most important sources of plant endophytes - colonization from external sources and vertical transmission via seeds. Total genomic DNA was isolated from rice seeds and bacterial DNA was selectively amplified by PCR. The diversity of endophytic bacteria was studied through Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis. Diversity between the original parents and the pure inbred line may show significant differences in terms of richness, evenness and diversity indices. Heat maps reveal astonishing contributions of both or either parents (IR29 x Pokkali and AT401 x IR31868) in the shaping of the bacterial seed endophytes of the succeeding pure inbred line from the original hybrid host. Most of the T-RFs of the subsequent pure inbred line (FL478 and IC32) could be traced to any or both of the parents. Comparison of common and genotype-specific T-RFs of parents and their offspring reveals that majority of the T-RFs are shared suggesting higher transmission of bacterial communities common to both parents. The parents influence the bacterial community of their offspring. Unique T-RFs of the offspring also suggest external sources of colonization particularly as the seeds are cultivated in different ecogeographical locations. This study showed that host parental lines contributed greatly in the shaping of bacterial seed endophytes of their offspring. It also revealed transmission and potential conservation of core seed bacterial endophytes that generally become the dominant microbiota in the succeeding generations of plant hosts.
Denver I. Walitang,Tongmin Sa(사동민) 한국토양비료학회 2021 한국토양비료학회 학술발표회 초록집 Vol.2021 No.11
Inoculation with the endophytic plant growth promoting bacteria (PGPB) leads to plant responses concurrent to the integration of the bioinoculum to the native endophytic bacterial community of the host plant. The interaction of the bioinoculum on the community of the native endophytic bacteria of the host plant is also understudied but should be an essential factor when assessing effects of bioinoculants. The study evaluated the modulation of host defense responses in rice when it is inoculated by Methylobacterium oryzae CBMB20. The effect of inoculation on the diversity and community structure of the root and shoot bacterial endophytes in Oryza sativa L. spp. indica cv. IR29 was also investigated. The defense responses observed in IR29 measured in terms of ROS and PR protein production significantly increased, but was eventually modulated by Methylobacterium oryzae CBMB20. Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) analysis, CBMB20 was shown to integrate with the native endophytic bacterial community of rice without causing significant changes in the bacterial diversity and community structure. The endophytic bacterial community of the rice seedling could be traced to the original seed community suggesting that they are seed-borne endophytes. The dominant bacterial communities in rice are mainly represented by bacterial genera associated to Microbacterium, Delftia, Pseudomonas, Xanthomonas and Stenotrophomonas, Herbaspirillum, Enterobacter and Sphingomonas. In addition, Curtobacterium, Enterobacter, Stenotro-phomonas and Xanthomonas were observed in both the root and shoot community. The results showed that Methylobacterium oryzae CBMB20 could modulate defense responses and non-antagonistically integrate with the native endophytic bacterial community of the rice host opening another area on the mechanism of plant growth promotion by the bioinoculum.
Benefits of Bio-inoculant Immobilization Process for the Development of Biofertilizers
Mak Chanratana, Ki-Yoon Kim, Walitang Denver, Sandipan Samaddar, Poulami Chatterjee,Shamim Ahmed, Yeon-Gyeong Kang, Sun-Young Jeon, Chae-Eun Pyo, and Tong-Min Sa 忠北大學校 農業科學硏究所 2016 農業科學硏究 Vol.32 No.2
Meeting the society’s increasing demand for food and energy require sustainable and readily�ᅠ�ᅠ available sources. The green revolution has been instrumental in creating sufficient crop productivity and maintained soil fertility. In the context of sustainable agriculture, the utilization of biofertilizers is of paramount importance. Commercial biofertilizers (bacteria/fungi) and their association directly or indirectly provide a beneficial effect on plant growth by increasing the supply and/or availability of nutrients to the host plant. However, large scale and field application of plant growth promoting microorganisms (PGPMs) as bioinoculant are still limited. Some of the major limitations are related to poor quality, short shelf-life and the lack of applicable formulation for bioinoculant. Immobilization using polymer matrix has been promising in enhancing protection, shelf life and slow release of bioinoculants. Furthermore, bacterial growth after immobilization in the polymer matrix is promoted with the supplementation of essential nutrients. The present review highlights the beneficial impacts of liquid formulation and exploiting alginate immobilized PGPMs for eco-friendly and sustainable crop production.
Kyungmok Lee(이경목),Denver Walitang,Tongmin Sa(사동민) 한국토양비료학회 2021 한국토양비료학회 학술발표회 초록집 Vol.2021 No.11
Plant growth promoting bacteria (PGPB) that can colonize the rhizosphere, endosphere, and phyllosphere of crops can help to enhance plant growth, nutrient mobilization, yield, and tolerance to biotic and abiotic stress. The increase in global population to an estimated 9.7 billion by 2050 necessitates the increase in food production and consumption. This increases our reliance to conventional yet unsustainable practices. The use of microbials as substitute for chemical fertilizers is an ecofriendly and sustainable way of increasing crop yield with lesser impact to the environment in support to the provisions of the Paris Climate Change Accord. PGPB with endophytic ability could colonize internal plant tissues and provide direct beneficial effects to the plants. The use of GPF tagging has been proven as a meaningful molecular confirmation to evaluate internal colonization of bacteria in the plant tissues. This study aims to evaluate endophytic colonization of two PGPB Pseudomonas strains and assess their plant growth promotion on onion, green onion and zucchini. For endophytic colonization, Pseudomonas frederiksbergensis OS261 and Pseudomonas vancouverensis OB155 were gfp-tagged through triparental mating with the donor E. coli strain pFAJ1820 and helper E. coli strain pRK2013. Confocal microscopy was used to evaluate the colonization and intercellular localization of the bacteria. We observed that Pseudomonas OB155 and OS261 successfully colonized the plant tissues. To evaluate the plant growth promotion of Pseudomonas OB155 and OS261 wild type, seeds of green onion, onion and zucchini were seed bacterized and grown in growth pouches under gnotobiotic conditions in plant growth chamber. Pseudomonas OB155 and OS261 have beneficial traits such as indole acetic acid (IAA) production, 1-aminocyclopropane-1-carboxylate (ACC) deaminase, salicyclic acid, phosphate solublization. Bacterial inoculated seeds had enhanced plant growth particularly root length and dry weight. The seeds which were inoculated with high indole acetic acid (IAA) producing bacteria, showed beneficial effect on plant growth during early seedling stage.