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Ha Thi Thanh Tran,Duc Anh Truong,Viet Duc Ly,Hao Thi Vu,Tuan Van Hoang,Chinh Thi Nguyen,Nhu Thi Chu,Vinh The Nguyen,Duyen Thuy Nguyen,Kohtaroh Miyazawa,Takehiro Kokuho,Hoang Vu Dang 대한백신학회 2020 Clinical and Experimental Vaccine Research Vol.9 No.1
Purpose: To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease. However, the efficacy of these vaccines to protect the pig against field CSF strains needs to be considered, based on circulating strains of classical swine fever virus (CSFV). Materials and Methods: Recombinant E2-CSFV protein produced by baculovirus/insect cell system was analyzed by western blots and immunoperoxidase monolayer assay. The effect of CSFV-E2 subunit vaccines was evaluated in experimental pigs with three genotypes of CSFV challenge. Anti-E2 specific and neutralizing antibodies in experimental pigs were analyzed by blocking enzyme-linked immunosorbent assay and neutralization peroxidize-linked assay. Results: The data showed that CSFV VN91-E2 subunit vaccine provided clinical protection in pigs against three different genotypes of CSFV without noticeable clinical signs, symptoms, and mortality. In addition, no CSFV was isolated from the spleen of the vaccinated pigs. However, the unvaccinated pigs exhibited high clinical scores and the successful virus isolation from spleen. These results showed that the E2-specific and neutralizing antibodies induced by VN91-E2 antigen appeared at day 24 after first boost and a significant increase was observed at day 28 (p<0.01). This response reached a peak at day 35 and continued until day 63 when compared to controls. Importantly, VN91-E2 induced E2-specific and neutralizing antibodies protected experimental pigs against high virulence of CSFVs circulating in Vietnam, including genotype 1.1, 2.1, and 2.2. Conclusion: These findings also suggested that CSFV VN91-E2 subunit vaccine could be a promising vaccine candidate for the control and prevention of CSFV in Vietnam.
Tran Ha Thi Thanh,Dang Anh Kieu,Ly Duc Viet,Vu Hao Thi,Hoang Tuan Van,Nguyen Chinh Thi,Chu Nhu Thi,Nguyen Vinh The,Nguyen Huyen Thi,Truong Anh Duc,Pham Ngoc Thi,Dang Hoang Vu 아세아·태평양축산학회 2020 Animal Bioscience Vol.33 No.10
Objective: The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control and preventive measures of ASF. Some methods have been recommended by FAO/OIE to detect ASFV in clinical samples, including real-time polymerase chain reaction (PCR). However, mismatches in primer and probe binding regions may cause a false-negative result. Here, a slight modification in probe sequence has been conducted to improve the qualification of real-time PCR based on World Organization for Animal Health (OIE) protocol for accurate detection of ASFV in field samples in Vietnam. Methods: Seven positive confirmed samples (four samples have no mismatch, and three samples contained one mutation in probe binding sites) were used to establish novel real-time PCR with slightly modified probe (Y = C or T) in comparison with original probe recommended by OIE. Results: Both real-time PCRs using the OIE-recommended probe and novel modified probe can detect ASFV in clinical samples without mismatch in probe binding site. A high correlation of cycle quantification (Cq) values was observed in which Cq values obtained from both probes arranged from 22 to 25, suggesting that modified probe sequence does not impede the qualification of real-time PCR to detect ASFV in clinical samples. However, the samples with one mutation in probe binding sites were ASFV negative with OIE recommended probe but positive with our modified probe (Cq value ranked between 33.12-35.78). Conclusion: We demonstrated for the first time that a mismatch in probe binding regions caused a false negative result by OIE recommended real-time PCR, and a slightly modified probe is required to enhance the sensitivity and obtain an ASF accurate diagnosis in field samples in Vietnam.
Thi Kim Anh VU,Bich Ha DAM,Thi Thuy Van HA 한국유통과학회 2022 유통과학연구 Vol.20 No.1
Purpose This research aims to investigate factors affecting the application of strategic management accounting in Vietnamese Logistics Enterprises. Design/Methodology/ Approach: Quantitative research was conducted and data was collected by sending questionnaires to 188 accountants and directors from Vietnamese Logistics Enterprises (including, transportation, warehousing and forwarding). 5 factors (namely: size and organizational structure, technological advancement, and strategic management accounting implementing costs) were selected to measure the application of strategic management accounting (SMA) in Vietnamese Logistics Enterprises (LEs) through correlation and regression analysis. Results: The empirical findings show that there exists a significant association between these factors and the SMA application. LEs have strongly supported the application of SMA as a tool to provide information for making their strategic decisions. The factors including size and organizational structure, technological advancement, SMA implementing c osts, and strategy positively impact the SMA application in both financial and non-financial aspects. This finding helps the adm inistrators realize the importance of SMA. Conclusions: This study provided an overview of SMA application and its benefits to enterprises. It helps managers have a better understanding of SMA and future directions for application. Moreover, the research results will be useful for managers to identify factors influencing their SMA practices and improve the current management process applied in organizations.
Ha Thi Thu Vu,Hanh Thi Vinh Le,Yen Thi Hai Pham,Hung Quoc Le,Phong Hong Pham 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.3
Different kinds of materials for electrodes based on glassy carbon, carbon fibers, and modified by an ionic liquid (BMIMBF4) were used to determine 2,4,6-trinitrotoluene (TNT) in water environment by differential pulse adsorptive stripping voltammetry. The modified electrode based on BMIMBF4 showed a better performance than those based on the microelectrode and glassy carbon electrode (GCE) in term of sensitivity. The best performance was obtained with the ionic liquid-modified electrode with 80:10:10 (w/w/w) of the graphite powder/paraffin oil/BMIMBF4 composition in PBS, pH 8. The TNT concentration was linear in the range 1.5 to ~30 ppm for all studied electrodes (r = 0.9978) with a detection limit of 88.6 ppb using IL-CPE (ionic liquid-modified carbon paste electrode). This latter electrode was used to measure real samples taken from Red River, Vietnam. The recovery study for TNT in natural samples gave values from 99.7% to 103.3%. IL-CPE demonstrated long-term stability and reproducibility, with a relative standard deviation of 1.67%.
Thi Vu, Hong Ha,Atabaev, Timur Sh.,Ahn, Ji Young,Dinh, Nguyen Nang,Kim, Hyung-Kook,Hwang, Yoon-Hwae The Royal Society of Chemistry 2015 Journal of Materials Chemistry A Vol.3 No.20
<▼1><P>Phosphor particles were introduced as a luminescent medium to improve the overall efficiency of dye sensitized solar cells (DSSCs).</P></▼1><▼2><P>Phosphor particles were introduced as a luminescent medium to improve the overall efficiency of dye sensitized solar cells (DSSCs). In the preparation process, TiO2 and phosphor particles were mixed to make photoelectrodes with bilayer (TiO2/mix), 3-layer (TiO2/mix/TiO2) and 4-layer (TiO2/mix/TiO2/mix) structures. The cell with the bilayer structure (TiO2/mix) after treating with TiCl4 solution showed the highest light-to-electric energy conversion efficiency (8.78%), which was ∼25.8% higher than that of a cell with a pure TiO2 layer under the same experimental conditions. The improvement in the energy conversion efficiency of DSSCs was attributed to phosphor enhanced incident light-harvesting <I>via</I> up- and down-conversion luminescence processes, resulting in an increase in the photocurrent.</P></▼2>
Climate Change Issues of Paper Manufacturing Production at Phong Khe Craft Village
Ha, Vu Thi Hong,Ahn, Ji Whan The Korean Society for Energy 2018 에너지공학 Vol.27 No.1
Today, the development of industries and rapid urbanization has a profound impact on the human environment. It can be said that the environment in the craft village is most affected. The environment in these areas is severely degraded and depending on the type of production, the environment in the craft villages is different. In Phong Khe ward of Bac Ninh Province, Vietnam, there are over 204 small and large facilities of recycling and producing paper. The paper facilities produce about 200,000 tons of paper each year and the amount of paper produced is not less than 500 tons each day in Phong Khe. They are creating livelihoods for more than 6,000 workers and generating local budgets from VND- 50 billion to VND 60 billion per year. Despite this great contribution, the use of old machines and "backward" modes of production means that paper recycling has caused serious air, soil, and water pollution in the region. The specific objectives of this study were as follows: (1) provide an overview of paper production process in the Phong Khe paper craft village; (2) comprehensive literature review of the current status of water environment, atmosphere environment, soil environment and solid waste; (3) figure out about waste treatment in Phong Khe ward.
Vu, Hong Ha Thi,Hwang, Yoon-Hwae,Kim, Hyung-Kook Korea Photovoltaic Society 2016 Current Photovoltaic Research Vol.4 No.2
Increasing the efficiency of dye-sensitized solar cells (DSSCs) by the fabrication of new photoelectrodes (PEs) is an important challenge. This study examined the photovoltaic parameters of DSSCs composed of a $TiO_2$ PE with $WO_3$ nanoparticles (NPs). A number of PEs with the same thickness but different concentrations of $WO_3$ NPs in the $TiO_2PE$ were prepared. The morphology and structural properties of the prepared PEs were examined by field-emission scanning electron microscopy and X-ray diffraction, respectively. The effects of the $WO_3$ NPs mixing concentration on the efficiency of DSSCs were investigated under simulated solar light irradiation.
Building-up a capillary electrophoresis system having both UV and electrochemical detectors
Ha, Vu Thi Thu,Lee, In Ja 한국공업화학회 2004 응용화학 Vol.8 No.2
A home-built capillary electrophoresis system equipped with an on-column UV detector and a post-column amperometric detector is developed in order to separate and detect any sample having either optical activity or electrochemical activity. The system has been tested for aniline and phenols and aniline by Micellar Electrokinetic Capillary Chromatographic method. Absorption and electrochemical properties of the samples are also investigated.