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RISS 인기검색어
- 검색키워드
- 저자 : Venkatachalapathy, R.T. (검색결과 4 건)
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Bioresorbable whitlockite coatings on titanium by EPD for biomedical applications
S.P. Vinodhini,B. Venkatachalapathy,T.M. Sridhar 한양대학교 세라믹연구소 2016 Journal of Ceramic Processing Research Vol.17 No.9
We have developed biodegradable tri calcium phosphate [Ca3(PO4)2 , β-TCP (whitlockite)] coatings on titanium by surfacemodification to improve the corrosion resistance and biocompatibility. It is a bioresorbable biomaterial and is absorbed forthe formation of new bone tissues. Nano whitlockite powder was synthesized by wet chemical precipitation method and coatedon titanium surfaces by electrophoretic deposition (EPD) from a 1.5% suspension in ethanol. The coatings were carried outat various potentials ranging from 10-50 V and were followed by sintering in vacuum at 800 oC for 1 hr. The crystallinity,vibrational states, surface morphology and composition of the coatings were characterized by XRD, FTIR, and FE-SEM withEDAX respectively. The corrosion resistance of the coating was further evaluated electrochemically to study the stability of thecoatings using open circuit potential-time measurements, potentiodynamic polarization studies and impedance behavior in Ringer’ssolution. The electrochemical corrosion parameters evaluated indicate a shift towards noble direction for the whitlockite coatedsamples in comparison with uncoated titanium metal. Electrochemical impedance spectroscopic investigations revealed the stablenature of the coatings formed. In vitro cytotoxicity studies of the coated material were carried out by MTT assay. The stable natureof the cells confirms the biocompatibility of whitlockite coatings on the titanium substrate.
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Identification of a Novel Single Nucleotide Polymorphism in Porcine Beta-Defensin-1 Gene
Pruthviraj, D.R.,Usha, A.P.,Venkatachalapathy, R.T. Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.3
Porcine beta-defensin-1 (PBD-1) gene plays an important role in the innate immunity of pigs. The peptide encoded by this gene is an antimicrobial peptide that has direct activity against a wide range of microbes. This peptide is involved in the co-creation of an antimicrobial barrier in the oral cavity of pigs. The objective of the present study was to detect polymorphisms, if any, in exon-1 and exon-2 regions of PBD-1 gene in Large White Yorkshire (LWY) and native Ankamali pigs of Kerala, India. Blood samples were collected from 100 pigs and genomic DNA was isolated using phenol chloroform method. The quantity of DNA was assessed in a spectrophotometer and quality by gel electrophoresis. Exon-1 and exon-2 regions of PBD-1 gene were amplified by polymerase chain reaction (PCR) and the products were subjected to single strand conformation polymorphism (SSCP) analysis. Subsequent silver staining of the polyacrylamide gels revealed three unique SSCP banding patterns in each of the two exons. The presence of single nucleotide polymorphisms (SNPs) was confirmed by nucleotide sequencing of the PCR products. A novel SNP was found in the 5'-UTR region of exon-1 and a SNP was detected in the mature peptide coding region of exon-2. In exon-1, the pooled population frequencies of GG, GT, and TT genotypes were 0.67, 0.30, and 0.03, respectively. GG genotype was predominant in both the breeds whereas TT genotype was not detected in LWY breed. Similarly, in exon-2, the pooled population frequencies of AA, AG, and GG genotypes were 0.50, 0.27, and 0.23, respectively. AA genotype was predominant in LWY pigs whereas GG genotype was predominant in native pigs. These results suggest that there exists a considerable genetic variation at PBD-1 locus and further association studies may help in development of a PCR based genotyping test to select pigs with better immunity.
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E. Manikandan,K. Velmurugan,G.G. Sozhamannan,Balamuruga Mohan Raj.G,V.S.K. Venkatachalapathy 한양대학교 청정에너지연구소 2023 Journal of Ceramic Processing Research Vol.24 No.6
Grey relational analysis of the multiple responses, including Reynolds number, Nusselt number, heat transfer (W), heattransfer coefficient (W/m2 K), and heat flux (W/m2), was done as part of experiments based on a Taguchi orthogonal array. The best parameter levels was calculated and selected based on GRA on basis of the Grey Relational Grade (GRG). AnANOVA has been used to identify factors that significantly contribute to the response based on grade. Experiments was conductedat the optimum predicted conditions (Al2O3) and the response was found as follows; Reynolds Number (592), NusseltNumber (2.5), Heat transfer (Q) (510), Flux (W/m2) (17.8) and Heat transfer Coefficient (W/m2 K) (394). The experimentalvalue was found to be correlated with the predicted values of Reynolds number (610), Nusselt number (2.6), heat transfer (Q)(530), heat transfer coefficient (W/m2 K) (415), and heat flux (W/m2) (18.5), respectively. The experimental data were foundto have an excellent knowledge under the recommended conditions. Our study is a preliminary step towards developing asubstantially effective Al2O3-based base fluid to enhance the performance of the heat transfer process in a copper-based serpentineshape heat exchanger
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