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Toxicity of TiO2 nanoparticles on the NRK52E renal cell line
Xavier Valentini,Lara Absil,Guy Laurent,Alexandre Robbe,Sophie Laurent,Robert Muller,Alexandre Legrand,Denis Nonclercq 대한독성 유전단백체 학회 2017 Molecular & cellular toxicology Vol.13 No.4
Titanium dioxide nanoparticles are widely used in industry to produce a number of products (cosmetics, paints, foods). TiO2 nanoparticles form aggregates and their toxicity varies in function of their size. In this study, aggregate sizes were determined using dynamic light scattering (DLS). The stability of the suspension was evaluated by turbidimetry and the size by transmission electronic microscopy (TEM). In the present study the toxicity of TiO2 nanoparticles was evaluated in vitro on rat kidney proximal tubular cells(NRK-52E). Cells were exposed to TiO2 nanoparticles (20 μg/mL) for 24, 48, 72 and 96 hours. TiO2 nanoparticles induce the production of reactive oxygen species(ROS). The relative number of mitosis decreased, while an increase of apoptotic cells was noted. The number of S-phase cells evidenced by BrdU immunoreactivity in cultures was significantly reduced by the exposure to the nanoparticles. These results attest to the toxic effect of the TiO2 nanoparticles on NRK-52E cells.
Underground Economy, Evasion and Inequality
Enzo Valentini 한국국제경제학회 2009 International Economic Journal Vol.23 No.2
Economic theory is paying increasing attention to a non-observed economy (NOE) and its causes. Recently, a couple of works (Rosser et al., 2000, 2003) have claimed that there is a positive relationship between income inequality and the size of NOE. This supposed relationship is not so clear and deserves in-depth analysis. There is a crucial aspect that has been completely avoided in these studies: income inequality is mainly measured using 'regular' incomes and this fact could lead to some bias. The existence of a certain size of NOE implies some income evasion that can affect the inequality indexes used in the study of the relationship between NOE and inequality. Including the regional share of NOE in a wage equation, I find that, in the specific case of the Italian private sector employees, the income evasion attached to NOE tends to reduce inequality measured by regular wages statistics.
Stabilization of Rat Serum Proteins Following Oral Administration of Fish Oil
Saso, Luciano,Valentini, Giovanni,Mattei, Eleonora,Panzironi, Claudio,Casini, Maria Luisa,Grippa, Eleonora,Silvestrini, Bruno The Pharmaceutical Society of Korea 1999 Archives of Pharmacal Research Vol.22 No.5
The mechanism of action of fish oil (FO), currently used in different chronic inflammatory conditions such as rheumatoid arthritis (RA), is not completely understood, although it is thought that it could alter the metabolism of endogenous autacoids. In addition, we hypothesized that the known capability of fatty acids (FA) of stabilizing serum albumin and perhaps other proteins, may be of pharmacological relevance considering that it is shared by other anti-rheumatic agents (e.g. nonsteroidal antiinflammatory drugs). Thus, we studied the effect of oral administration of FO and corn oil (CO), a vegetable oil with a different composition, on the stability of rat serum proteins, evaluated buy a classical in vitro method based on heat-induced protein denaturation. FO, and, to a lower extent, CO inhibited heat-induced denaturation of rat serum (RS): based on the inhibitory activity (EC50) of the major fatty acids against heat-induced denaturation of RS in vitro, it was possible to speculate the in vivo effects of palmitic acid (C16:0) and eicosapentaenoic acid (EPA, C20:5, n-3) may be more relevant than that of linolenic acid (C18:2). To better investigate this phenomenon, we extracted albumin from the serum of animals treated or not with FO with a one-step affinity chromatography technique, obtaining high purity rat serum albumin preparations (RSA-CTRL and RSA-FO), as judged by SDS-PAGE with Coomassie blue staining. When these RSA preparations were heated at $70^{\circ}C$ for 30 min, it was noted that RSA-FO was much more stable than RSA-CTRL, presumably due to higher number of long chain fatty acids (FA) such as palmitic acid or EPA. In conclusion, we provided evidences that oral administration of FO in the rat stabilizes serum albumin, due to an increase in the number of protein bound long chain fatty acids (e.g. palitic acid and EPA). We speculate that the stabilization of serum albumin and perhaps other proteins could prevent changes of antigenicity due to protein denaturation and glycosylation, which may trigger pathological autoimmune responses, suggesting that this action may be involved in the mode of action of FO in RA and other chronic inflammatory diseases.
Fluorescence Enhancement of a Microbial Rhodopsin via Electronic Reprogramming
Marí,n, Marí,a del Carmen,Agathangelou, Damianos,Orozco-Gonzalez, Yoelvis,Valentini, Alessio,Kato, Yoshitaka,Abe-Yoshizumi, Rei,Kandori, Hideki,Choi, Ahreum,Jung, Kwang-Hwan,Haacke, Stefan American Chemical Society 2019 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.141 No.1
<P>The engineering of microbial rhodopsins with enhanced fluorescence is of great importance in the expanding field of optogenetics. Here we report the discovery of two mutants (W76S/Y179F and L83Q) of a sensory rhodopsin from the cyanobacterium <I>Anabaena</I> PCC7120 with opposite fluorescence behavior. In fact, while W76S/Y179F displays, with respect to the wild-type protein, a nearly 10-fold increase in red-light emission, the second is not emissive. Thus, the W76S/Y179F, L83Q pair offers an unprecedented opportunity for the investigation of fluorescence enhancement in microbial rhodopsins, which is pursued by combining transient absorption spectroscopy and multiconfigurational quantum chemistry. The results of such an investigation point to an isomerization-blocking electronic effect as the direct cause of instantaneous (subpicosecond) fluorescence enhancement.</P> [FIG OMISSION]</BR>