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- 저자 : Ur Rahman M. Ashiq (검색결과 1 건)
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Mohiadeen Batcha M.,Sajith Ahamed A.,Ur Rahman M. Ashiq,Janarthanan S.,Krishnan M. 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2
The present study aims to focus on the site of fat bodies involved in the differential expression of vitellogenin protein in Bombyx mori L which is not yet fully understood. Culturing fat bodies and analyzing in in vitro and in vivo approaches made it useful to study this pattern. In Bombyx mori, L. The vitellogenin protein is 200–205 kDa and resolves at two molecular weight regions that are at 180 kDa and 42 kDa. We subject dorsal perivisceral (DPV) and ventral perivisceral fat body (VPV), haemolymph for this radio labeling cell culture and incorporation experiment. Based on our in vitro and in vivo experiments of S 35 methionine fluorography, Scanning densito metric, Western blot analysis and RT-PCR expression studies had strongly proven that the ventral perivisceral fat body is the significant and exclusive site of differential vitellogenin expression in silkworm, Bombyx mori but not in dorsal peripheral fat body tissues, haemolymph by radiolabeling studies. The present paper describes an experimental set-up based on the incubation of ventral perivisceral and dorsal perivisceral fat bodies and the measurement of vitellogenin with S 35 protein labeling incorporation method. These findings from our lab were the first report proved by in vitro and in vivo labeling experiments.
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