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      • Parasitoids of Riptortus pedestris (Hemiptera: Alydidae) and their use in conservational biological control

        Un Taek Lim 한국응용곤충학회 2017 한국응용곤충학회 학술대회논문집 Vol.2017 No.04

        Riptortus pedestris (Fabricius) is one of major soybean pests in Korea. Its feeding during pod formation period significantly reduces the yield of soybean. Management tactics commonly used include insecticide such as etofenprox and aggregation pheromone trap, but they have been often found ineffective. As an alternative methods, parasitoids such as Gryon japonicum (Ashmead) and Ooencyrtus nezarae Ishii could be conserved by directly releasing non-viable host eggs in the field, by incorporating the non-viable host eggs into aggregation pheromone trap, by planting a trap crop of natural enemies, or by combining selective insecticides with a trap crop of natural enemies. I present study results of these tactics with discussion here.

      • Demonstration of Interspecific Competition Between Two Sympatric Egg Parasitoids of Bean Bug to Explain Coexistence in Temporal Scales

        Un Taek Lim,Bishwo P. Mainali,Jaegeun Kim 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05

        Ooencyrtus nezarae Ishii and Gryon japonicum (Ashmead), egg parasitoids of Riptortus pedestris (Fabricius), coexist despite direct competition for host eggs. As asymmetrical pattern of seasonal occurrence, i.e., more G. japonicum during spring-summer and more O. nezarae during summer-fall, has been reported, host resource partitioning may occur in temporal scales. To test this hypothesis, we demonstrated the interspecific competition between the two species by measuring parasitism in nine combinations of host densities and exposure times. To reflect gregarious-solitary dichotomy, three O. nezarae and one G. japonicum mated females were used in each experiment. O. nezarae was better competitor when exposure time was longer than 1 day irrespective of host densities. Parasitism rate and progeny emergence of O. nezarae was 1.6-2.8 and 4.7-7.3 times higher than G. japonicum. O. nezarae has higher potential rate of increase than G. japonicum due to gregariousness, and be more successful in larval competition inside multiparasitized host egg as it acts as a facultative hyperparasitoid. Although G. japonicum was more effective in host finding (as they showed relatively higher per capita parasitism and progeny emergence), their progeny suffered high mortality from the larval competition with O. nezarae. These results may explain the asymmetrical occurrence pattern in the field.

      • Comparison of lethal concentration of mycoinsecticide against Riptortus pedestris (F.) and its parasitoid

        Naresh Dangi,Un Taek Lim 한국응용곤충학회 2015 한국응용곤충학회 학술대회논문집 Vol.2015 No.04

        Lethal concentration of Beauveria bassiana (Strain GHA, Botanigard, Arysta LifeScience) against Riptortus pedestris (F.) (Hemiptera: Alydidae) was compared with that of its’ parasitoid Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae). Five concentrations of the mycoinsecticide, i.e., 5×108, 1×108, 2×107, 4×106, and 8×105 conidia/ml, were used. Glass scintillation vial was coated with 100 μl solution, and insects were released and assessed up to 12 hours. The LC50 and LC20 of second instar R. pedestris were 1.8×1010 and 1.5×108 conidia/ml, and those of O. nezarae were 1.9×107 and 2.8×106 conidia/ml, respectively. The mortality of O. nezarae was observed 3.9 times higher than R. pedestris in higher concentration. This result shows that O. nezarae is more susceptible to the mycoinsecticide than R. pedestris.

      • Nymphal development and reproductive activity of Riptortus pedestris (Hemiptera: Alydidae) fed on soybean seeds and fruits of apple and sweet persimmon

        Eunmok Kim,Un Taek Lim 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10

        Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) recently become an important fruit-spotting bug of apple and sweet persimmon trees in Korea. Although fruits of cultivated species are generally known to be unsuitable food for the fruit-spotting bugs, dietary importance of fruits of apple and sweet persimmon for R. pedestris is unknown. We evaluated fruits of apple and sweet persimmon with or without soybean seeds as a food source for R. pedestris by investigating nymphal development time, nymphal mortality, preovipositional period, fecundity, and adult size in the laboratory. None of 1st instar R. pedestris fed only on a fruit of apple or sweet persimmon became second instar nymph. When provided with soybean seeds together, both the fruits did support the nymphal development and the reproduction of R. pedestris. However, diets including soybean and one of the fruits were not better than control diets consisting of soybean seeds with or without vitamin C. Therefore, apple and sweet persimmon are not essential food sources for the development and the reproduction of R. pedestris. These results suggest that R. pedestris may not reproduce on the fruit trees, thus explain absence of either their eggs or their nymphs on the fruit trees in the fields.

      • Seeds of Leguminous Plants, Acacia and Mung Bean as Food Sources of Bean Bug, Riptortus pedestris (Hemiptera: Alydidae)

        Eunmok Kim,Un Taek Lim 한국응용곤충학회 2013 한국응용곤충학회 학술대회논문집 Vol.2013 No.04

        The bean bug, Riptortus pedestris (F.) (Hemiptera: Alydidae) is one of the most important polyphagous pests in soybean in Korea and Japan. With the review of previous studies on the suitability of different diets, we additionally tested two leguminous plants as food sources of R. pedestris by assessing development and reproduction of the bugs. The tested diets include seeds of acacia, two mung bean varieties (var. Eoul and Dahyeon), and soybean (var. Daewon). Riptortus pedestris fed on mung bean var. Eoul showed higher nymphal mortality than those fed on mung bean var. Dahyeon, acacia, and soybean. On acacia, development time and preoviposition period of R. pedestris was shortest among the tested diets Also, five day fecundity the highest in bugs fed on acacia seeds. These results showed that acacia seed can be a good quality diet for R. pedestris, and both acacia and mung bean could be a suitable host resources for R. pedestris during the period when soybean, a major host crop, is unavailable.

      • Within Plant Distribution of Riptortus clavatus (Hemiptera: Alydidae) Eggs and its Parasitoids in Soybean Fields

        Sangwon Kim,Un Taek Lim 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

        To understand microhabitat distribution of insect pest is important for developing timely management tactics. We investigated within plant distribution of Riptortus clavatus Thunberg (Hemiptera: Alydidae) eggs and its’ parasitism by egg parasitoids such as Ooencyrtus nezarae Ishii (Hymenoptera: Encyrtidae) in soybean fields. Sampling was carried out to determine the most preferred oviposition site (upper leaf surface, under leaf surface, pod and stem) in different varieties of soybean (Daewon, Cheongja, Taegwang, Daepung, Seomoktae, Seoritae) in Andong, 2007 and 2008. Parasitism was recorded only in 2008. In seasonal total, proportion of R. clavatus eggs deposited within soybean plant was significantly higher on under leaf surface (70-97%) followed by upper leaf surface, pod, and stem (2-29%, 0-8% and 0-2%, respectively) depending on varieties of soybean. In seasonal total, parasitism rate by O. nezarae was significantly higher on the eggs collected from under leaf surface (61%) followed by eggs collected from upper surface of the leaves (43%), pods (7%) and stem (33%). In conclusion, R. clavatus prefer upper leaf surface the most as a oviposition substrate in soybean, and highest parasitism by O. nezarae on the host eggs was also found on upper leaf surface.

      • Toxicity Evaluation of Different Plant Extract against Grapholita molesta (Lepidoptera: Tortricidae) in Laboratory Condition

        Souvic Sarker,Un Taek Lim 한국응용곤충학회 2016 한국응용곤충학회 학술대회논문집 Vol.2016 No.04

        Oriental fruit moth Grapholita molesta (Busck) (Lepidoptera: Tortricidae) is an important pest for stone fruits like apple, peach, pear etc. Growers usually depend on chemical insecticides to control this pest, but it causes problems like resistance, pest resurgence, and health hazard. Although various plant extracts have been known to be potential against other lepidopterans, their effectiveness against oriental fruit moth has rarely been studied. So, we evaluated toxicity of 28 methanolic plant extracts known from previous literatures in the laboratory condition. Residual toxicity against 1st instar larvae (<5 h old) were examined using glass scintillation vial method at the concentration of 2 mg/ml. Among the tested botanicals Nicotiana tabacum L. was most effective and showed 92% mortality within 20 h and LT50 value was 12.9 h followed by Allium sativum L., Zanthoxylum piperitum (L.), and Sapindus mukorossi Gaerten. which showed 88, 70, and 65% mortality within 20 h, respectively. Rhododendron micranthum Turcz., Humulus japonicus Seibold & Zucc., Nerium indicum Mill., and other extracts showed least effectiveness on 1st instar larvae of oriental fruit moth. Hence, we suggest N. tabacum, A. sativum, Z. piperitum, and S, mukorossi as botanicals to be used against oriental fruit moth.

      • Be Prepared: Winter Cherry Bug is Coming!

        Dongmok Kim,Un Taek Lim 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

        Winter cherry bug, Acanthocoris sordidus Thunberg (Hemiptera: Coreidae) is a pest of Solanaceous and Convolvulaceous crops and distributed in Taiwan, Japan, and Korea. Adult female generally lays eggs in cluster on host plant leaves. Most researches have been conducted in Japan, and it is regarded as a minor pest. In this study, we surveyed the seasonal population change of A. sordidus and its damages from May to September by visual count in experimental pepper fields located in Andong National University, where no pesticide was sprayed. Hatchability was measured using field-laid egg mass covered by nylon-meshed pouch. Potential disease transmission was confirmed in filed pepper fruits confined for a week with two adult A. sordidus inside nylon-meshed pouch. Adults and eggs of A. sordidus started to appear in 30th May and 10th June, respectively. Nymph populations were first found on 10th July. No eggs were found from 7th September when highest adult population (67/plant) was recorded. Hatchability was over 90% in the field. No disease transmission to pepper fruit was observed. We believe that A. sordidus would become a serious pest under a certain condition such as low level of insecticide use and climate change.

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