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Devendra Pandeya,Uck Kee Baek,Hak Soo Suh,Sang Gu Kang 한국육종학회 2006 한국육종학회지 Vol.38 No.4
Photoperiod sensitive genetic male sterile (PGMS) rice 920S was characterized as sterile when it was grown under longday condition but fertile when grown under short day condition. PGMS rice 920S, Fl and F2 plants were examined for their fer-tilities unde
Purification and biochemical characterization of a fibrinolytic enzyme from Bacillus subtilis BK-17
Jeong, Yong Kee,Park, Jeong Uck,Baek, Hyun,Park, Sung Hoon,Kong, In Soo,Kim, Dong Wan,Joo, Woo Hong Institute of Genetic Engineering Changwon National 2001 Gene and Protein Vol.5 No.-
A fibrinolytic enzyme from Bacillus subtilis BK-17 has been purified to homogeneity by gel-filtration and ion exchange chromatography. Compared to the crude enzyme extract, the specific activity of the enzyme increased 929-fold with a recovery of 29%. The subunit molecular mass of the purified enzyme was estimated to be 31 kDa by SDS-PAGE. The N-terminal amino acid sequence of the purified fibrinolytic enzyme was; A-Q-S-V-P-Y-G-V-S-Q-I-K-A-P-A-A-H-N. The sequence was highly homologous to the fibrinolytic enzymes nattokinase, subtilisin J and subtilisin E from bacillus spp. However, there was a substitution of three amino acid residues in the N-terminal sequence. The amidolytic activity of the purified enzyme for several substrates was assessed. In comparison with nattokinase and CK (fibrinolytic enzyme from a Bacillus spp.), which showed strong fibrinolytic activity, the amidolytic activity of the enzyme for the synthetic substrate, kallikrein (H-D-Val-Leu-Arg-pNA, S-2266) increased 2.4- and 11.8-fold, respectively.
Devendra Pandeya,서학수,강상구,Uck-Kee Baek 한국육종학회 2006 한국육종학회지 Vol.38 No.5
Photoperiod sensitive genetic male sterile (PGMS) rice 920S was characterized as sterile when it was grown under longday condition but fertile when grown under short day condition. PGMS rice 920S, F1 and F2 plants were examined for their fer-tilities under diferent photoperiods. All 920S parent plants were fertile in short photoperiod condition at 10 hrs day length, partialfertile in intermediate photoperiod condition at 13 hrs day length and sterile in long photoperiod condition at 14 hrs day length.antsshowed wide range of fertility with partial segregation of complete fertile, partial fertile, and complete sterile plants when theywere exposed to intermediate day length condition of 13 hrs day length. Therefore, here we suggest that there is a critical pho-toperiod to affect a PGMS gene that causes an alteration in fertility.